Biotransformation of l‐menthol by twelve isolates of soil‐borne plant pathogenic fungi (Rhizoctonia solani) and classification of fungi

The microbial transformation of l‐menthol ( 1 ) was investigated by using 12 isolates of soil‐borne plant pathogenic fungi, Rhizoctonia solani (AG‐1‐IA Rs24, Joichi‐2, RRG97‐1; AG‐1‐IB TR22, R147, 110.4; AG‐1‐IC F‐1, F‐4, P‐1; AG‐1‐ID RCP‐1, RCP‐3, and RCP‐7) as a biocatalyst. Rhizoctonia solani F‐1, F‐4 and P‐1 showed 89.7–99.9% yields of converted product from 1 , RCP‐1, RCP‐3, and RCP‐7 26.0–26.9% and the other isolates 0.1–12.0%. In the cases of F‐1, F‐4 and P‐1, substrate 1 was converted to (?)‐(1S,3R,4S,6S)‐6‐hydroxymenthol ( 2 ), (?)‐(1S,3R,4S)‐1‐hydroxymenthol ( 3 ) and (+)‐(1S,3R,4R,6S)‐6,8‐dihydroxymenthol ( 4 ), which was a new compound. Substrate 1 was converted to 2 and/or 3 by RRG97‐1, 110.4, RCP‐1, RCP‐3 and RCP‐7. The structures of the metabolic products were elucidated on the basis of their spectral data. In addition, metabolic pathways of the biotransformation of 1 by Rhizoctonia solani are discussed. Finally, from the main component analysis and the differences in the yields of converted product from 1 , the 12 isolates of Rhizoctonia solani were divided into three groups based on an analysis of the metabolites. Copyright © 2003 Society of Chemical Industry     

An automaton for noncontact testing of male threading

Translated from Izmeritel'naya Tekhnika, No. 9, pp. 31–32, September, 1991.     

Generating Future Land-Use and Transportation Plans for High-Growth Cities Using a Genetic Algorithm

Abstract:   An elitist genetic algorithm was used to find a diverse non-dominated set of optimal future zoning and street plans for two high-growth cities in the United States of America. Plans were judged with regard to housing capacity, employment capacity, greenspace, traffic congestion, and change from the status quo. A multiobjective fitness function was used. The genetic algorithm offers the possibility of efficiently searching over tens of thousands of plans for a trade-off set of non-dominated plans. The trade-off set ranged from a minimum change plan, where undeveloped farmland was rezoned as commercial or residential land, to a minimum traffic congestion plan where commercial and residential usage were spread throughout the cities rather than concentrated in one or two areas. The algorithm is general enough to be applied to other cities and metropolitan regions.     

Advanced material processing with nano- and femto-second pulsed laser

In order to apply laser processing to advanced material mold making, basic research is carried out. First, box-shaped hole processing is carried out by laser beam scanning. Second, suitable machining conditions are investigated in terms of laser spot size, scanning path, scanning speed and scan times. Finally, the comparison of nano- with femto-second laser-processed surfaces is carried out. The laser-processed surfaces and debris are observed with a scanning electron microscope (SEM) and a laser microscope, and are also analyzed using an electron probe microanalysis (EPMA), an X-ray diffractometer and a Vickers hardness tester.     

Novel front‐light system using fine‐pitch patterned OLED

Abstract— A novel front‐light system that uses an organic light‐emitting‐diode (OLED) light source patterned with a fine pitch has been developed. The front‐light system has the following characteristics: (1) excellent uniformity within the light‐emitting area; (2) emittance that is consistent at all viewing angles; (3) no light leakage at any viewing angle from the side of the observer. This system can be adopted for reflective LCDs, electrophoretic displays (EPDs), microelectromechanical systems (MEMS), and other applications.     

Maximum diameter of the rod-shaped specimen for transmission electron microtomography without the "missing wedge"

In our recent study, the complete rotation of a rod-shaped specimen during transmission electron microscopy (TEM) has been successfully carried out, yielding a truly quantitative three-dimensional (3D) structure of a ZrO(2)/polymer nano-composite. This result allows the further development of transmission electron microtomography (TEMT) for materials science. The diameter of the rod-shaped specimen was about 150 nm, which may not be statistically large enough to evaluate structural parameters, e.g., volume fraction of Zr nano-particles. Thus, it is preferable to image rods with larger diameters in 3D. In this study, several rod-shaped specimens whose diameters ranged from 150 to 530 nm were subjected to the "distortion-free TEMT". The maximum diameters, l, observable under 200 and 300 kV-TEMTs were, respectively, 460-470 and 600-670 nm (corresponding the maximum relative diameters, l/lambda (lambda: mean free path), were ca. 2.2 and 2.7-3.0).     

Acid-ageing of lignocellulosics to improve ruminant digestibility—application to bagasse,wheat and rice straw and oat hulls

Lignocellulosic agricultural wastes were impregnated with small amounts of hydrochloric and sulphuric acids and aged under varying conditions of temperature and time. Hydrochloric acid was added as vapour and sulphuric acid as a dilute aqueous solution and the fibres subsequently air-dried. The acid-aged fibres became more digestible to rumen fluid (in vitro) and to cellulase, with improvements up to four-fold (for 1.1% hydrochloric acid in oat hulls). The increases in rumen fluid digestibility were always associated with increases in water-solubility. The effects are attributed to acid hydrolysis, especially of hemicelluloses and perhaps to some extent of lignin–carbohydrate linkages. The procedures may have some potential for low-cost, small scale exploitation in upgrading lignocellulosic agricultural wastes for cattle fodder.     

Biotransformation of l‐menthol by soil‐borne plant pathogenic fungi (Rhizoctonia solani)

The biotransformation of l‐menthol was investigated by using nine isolates of Rhizoctonia solani (AG‐1‐IA Rs24, Joichi‐2, RRG97‐1; AG‐1‐IB TR22, R147, 110.4; AG‐1‐IC F‐1, F‐4 and P‐1) as a biocatalyst. In the cases of Rhizoctonia solani F‐1, F‐4 and P‐1, almost all of the substrate was consumed in 3 days and the major metabolite increased rapidly for the first of 3 days incubation. The structure of the major metabolite was elucidated on the basis of its spectral data. The major metabolite was determined to be (?)‐(1S,3R,4S,6S)‐6‐hydroxymenthol which indicated that l‐menthol was hydroxylated at the C‐6 position. From the main component analysis, the nine isolates of Rhizoctonia solani were divided into two groups based on their ability to transform l‐menthol to (?)‐(1S,3R,4S,6S)‐6‐hydroxymenthol. This is the first report on the biotransformation of l‐menthol by Rhizoctonia solani. © 2001 Society of Chemical Industry     

A study of three-dimensional shape machining with an ECμM system

This paper presents an electrochemical micromachining (ECμM) system developed with a machining gap control system. As a preliminary, electrochemical machining (ECM) experiments are carried out. The optimum machining condition of ECM is determined in terms of machining voltage, machining pulse length, amplitude of the electrode for flushing out contamination, and electrolyte concentration. After the preliminary ECM experiments, three-dimensional shape micromachining is carried out under the optimum condition. First, a prismatic electrode with a 200-μm square as the base shape is machined by ECM. Next, three-dimensional shape micromachining is carried out by scanning the prismatic electrode. A three-dimensional shape with sub-millimeter range is successfully machined.     

Transmission electron microtomography without the "missing wedge" for quantitative structural analysis

A three-dimensional (3D) visualization and structural analysis of a rod-shaped specimen of a zirconia/polymer nanocomposite material were carried out by transmission electron microtomography (TEMT) with particular emphasis on complete rotation of the specimen (tilt angular range: +/-90 degrees ). In order to achieve such an ideal experimental condition for the TEMT, improvements in the specimen as well as the sample holder were made. A rod-shaped specimen was necessary in order to obtain a high transmission of the specimen upon tilting to large angles. The image resolution of the reconstructed tomogram was isotropic, in sharp contrast to the anisotropic image resolution of the conventional TEMT with a limited angular range (the "missing wedge" problem). A volume fraction of zirconia, phi, evaluated from the 3D reconstruction was in quantitative agreement with the known composition of the nanocomposite. A series of 3D reconstructions was made from the tilt series with complete rotation by limiting the maximum tilt angle, alpha, from which a couple of structural parameters, the volume fraction and surface area per unit volume, Sigma, of the zirconia, were evaluated as a function of alpha. It was confirmed from actual experimental data that both phi and Sigma slightly decreased with the increasing alpha and reached constant values at around alpha=80 degrees , suggesting that the specimen may have to be tilted to +/-80 degrees for truly quantitative measurements.