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2.
Emine Kaplanoglu Igor Kolotilin Rima Menassa Cam Donly 《International journal of molecular sciences》2022,23(7)
Plant-mediated RNA interference (RNAi) holds great promise for insect pest control, as plants can be transformed to produce double-stranded RNA (dsRNA) to selectively down-regulate insect genes essential for survival. For optimum potency, dsRNA can be produced in plant plastids, enabling the accumulation of unprocessed dsRNAs. However, the relative effectiveness of this strategy in inducing an RNAi response in insects using different feeding mechanisms is understudied. To investigate this, we first tested an in vitro-synthesized 189 bp dsRNA matching a highly conserved region of the v-ATPaseA gene from cotton mealybug (Phenacoccus solenopsis) on three insect species from two different orders that use leaf-chewing, lacerate-and-flush, or sap-sucking mechanisms to feed, and showed that the dsRNA significantly down-regulated the target gene. We then developed transplastomic Micro-tom tomato plants to produce the dsRNA in plant plastids and showed that the dsRNA is produced in leaf, flower, green fruit, red fruit, and roots, with the highest dsRNA levels found in the leaf. The plastid-produced dsRNA induced a significant gene down-regulation in insects using leaf-chewing and lacerate-and-flush feeding mechanisms, while sap-sucking insects were unaffected. Our results suggest that plastid-produced dsRNA can be used to control leaf-chewing and lacerate-and-flush feeding insects, but may not be useful for sap-sucking insects. 相似文献
3.
Edita Zubrytė Danutė Kaušpėdienė Romas Ragauskas Rima Binkienė Aušra Selskienė 《分离科学与技术》2020,55(16):2855-2868
ABSTRACT A non-hazardous groundwater treatment waste (GWTW) was examined as a low-cost sorbent for Pb(II) and Cu(II) ions. The content of the dominant elements in GWTW was as follows: 78% Fe2O3, 7.4% P2O5, 7.4% CaO and 5.2% SiO2. The removal of Pb(II) and Cu(II) was fast, and more than 67–95% of ions were accumulated by GWTW during the first 3 min. The sorption capacity of GWTW depends on solution pH, concentration and temperature. Equilibrium data fitted well with Langmuir–Freundlich and Langmuir-partition models. The inherently formed nano-adsorbent could be utilized for the treatment of water contaminated with Pb(II) and Cu(II) ions. 相似文献
4.
Rima G Satgé J Dagiral R Lion C Sentenac-Roumanou H Fatôme M Roman V Laval JD 《Metal-Based Drugs》1999,6(1):49-60
The present review describes the work carried out during the last 20 years in the field of the radioprotective activity and toxicity of several classes of organosilicon and organogermanium compounds (i.e. metallathiazolidines, metalladithioacetals, metallatranes and germathianes). 相似文献
5.
Adsorption of anionic dyes on the cross-linked with epichlorohydrin starches containing quaternary ammonium groups (CCS) was
investigated and compared with that of modified starches containing only quaternary ammonium groups (CS). The adsorption of
anionic dyes on CS and CCS closely follows the Langmuir model of adsorption. The computed Langmuir saturation capacity Q
o increases with increasing degree of substitution (DS) of CS or CCS and has the value from 0.81 mol/kg to 3.22 mol/kg for
CCS and from 0.88 mol/kg to 1.87 mol/kg for CS. The effectiveness of the cationic groups in dye binding was about 1 mol/equiv
for CSS with DS from 0.47 to 0.62 and all the cationic groups had attached one anionic groups of the dye. Increasing DS of
CS decreases the effectiveness of dye binding due to the increase in solubility of CS, and the soluble part of CS binds the
dye as a typical soluble polyelectrolyte. CCS are more suitable than CS for the anionic dye adsorption from a textile dyeing
solution. DS of CCS should be about 0.5–0.6. They adsorb the anionic dyes in few minutes and acts in the wide range of pH
of the solutions. The Langmuir saturation capacity Q
o and the effectiveness of the cationic groups in dye binding increase with an increase of the adsorption temperature. The
positive values of the enthalpy and entropy suggest that the adsorption is endothermic and during the adsorption of the anionic
dye on CCS the randomness of the system increases. 相似文献
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7.
Sumac plant (Rhus typhina) is mainly used for forestation and gardening, whereas its fruit can be used to prepare a kind of beverage called “sumac-ade” and to treat gastrointestinal disorders. This study aimed at assaying the antimicrobial and antioxidant activities of R. typhina growing in China. The antimicrobial activity of R. typhina fruit extract was tested against twelve strains including Gram-positive and Gram-negative bacteria as well as yeasts. The extract showed a strong antimicrobial activity with a concentration-dependence and a broad antimicrobial spectrum for all tested bacteria species. Bacillus cereus and Helicobacter pylori were found to be the most sensitive Gram-positive and Gram-negative bacteria respectively, with a minimum inhibitory concentration (MIC) of 0.10%. However, yeasts exhibited much lower sensitivity, with MICs of 0.60–0.75%. Furthermore, the antioxidant activity of the extract was investigated, including scavenging activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals (0.016 mg/ml as IC50 value) and reducing power (IC50 value of 0.041 mg/ml). To our knowledge, this is the first report on the biological activities of R. typhina fruit extract and our findings suggest the possibility of using the fruit of R. typhina as a novel source of natural antimicrobial and antioxidant agents for the food and pharmaceutical industries. 相似文献
8.
By analysing the nucleotide sequence of the SH genes of five mumps virus strains derived from the clinical specimens collected during the 1995/96 mumps epidemic in China a new genotype has been established. The circulating viruses showed divergence ranging from 0.8-4.5% at the nucleotide level and 3.5-12.3% at the amino acid level. In addition, a more rational approach has been taken in proposing genotype groupings to MuV strains. 相似文献
9.
Rima Biswas Tamal Banerjee Pallab Ghosh Sheikh Musharaf Ali 《American Institute of Chemical Engineers》2019,65(9):e16686
Stripping of metal ions (i.e., Cs+ and Na+) in presence of ionophore such as dibenzo-18-crown-6, (DB18C6) from the ionic liquid phase to the aqueous nitric acid phase by molecular dynamics simulation is reported. The experimentally determined stripping percentages of Na+ (i.e., 43.4, 38.5, 34.4, and 31.9%) were found to be higher than the same for Cs+ (i.e., 32.6, 32.0, 31.3, and 30.2%). The nonbonded and the hydrogen bond energies between Na+ and water (i.e., −356.41 and −363.77 kcal/mol) were higher when compared with Cs+ (i.e., −212.43 and −221.04 kcal/mol). The spatial distribution functions further confirmed that the surfaces of Na+ were very closely distributed around the active sides of water whereas for Cs+, it was distributed very far from the water molecules. In the penultimate section, the effect of methanol to the aqueous phase was studied so as to enhance the extraction efficiency of the complex. 相似文献
10.
Lina Jarboui Yannick Deville Shahram Hosseini Rima Guidara Ahmed Ben Hamida Leonardo T. Duarte 《Multidimensional Systems and Signal Processing》2018,29(3):1153-1172
In this paper, we are interested in the problem of Blind Source Separation using a Second-order Statistics (SOS) method in order to separate autocorrelated and mutually independent sources mixed according to a bilinear (BL) model. In this context, we propose a new approach called Bilinear Second-order Blind Source Separation, which is an extension of linear SOS methods, devoted to separate sources present in BL mixtures. These sources, called extended sources, include the actual sources and their products. We first study the statistical properties of the different extended sources, in order to verify the assumption of identifiability when the actual sources are zero-mean and when they are not. Then, we present the different steps performed in order to estimate these actual centred sources and to extract the actual mixing parameters. The obtained results using artificial mixtures of synthetic and real sources confirm the effectiveness of the new proposed approach. 相似文献