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排序方式: 共有121条查询结果,搜索用时 15 毫秒
1.
Pasquale Stano Tereza Pereira de Souza Paolo Carrara Emiliano Altamura Erica D’Aguanno Margherita Caputo 《先进材料力学与结构力学》2015,22(9):748-759
Here we report some recent biophysical issues on the preparation of solute-filled lipid vesicles and their relevance to the construction of “synthetic cells.” First, we introduce the “semi-synthetic minimal cells” as the liposome-based cell-like systems, which contain a minimal number of biomolecules required to display simple and complex biological functions. Next, we focus on recent aspects related to the construction of synthetic cells. Emphasis is given to the interplay between the methods of synthetic cell preparation and the physics of solute encapsulation. We briefly introduce the notion of structural and compositional “diversity” in synthetic cell populations. 相似文献
2.
Cypriano G da Trindade Neto Ana LP Fernandes Ana IB Santos Waldenice A Morais Marcos VM Navarro Tereza NC Dantas Mrcia R Pereira Jos LC Fonseca 《Polymer International》2005,54(4):659-666
Complexation of chitosan in aqueous solutions by low molecular weight electrolytes is one of the simplest methods for the preparation of aqueous chitosan dispersions. In this work, the influence of storage time, sulfate concentration, method of preparation and surfactant content on some properties of the resultant chitosan dispersions (turbidity, viscosity and zeta potential) was analyzed. Turbidimetry was adequate to monitor the formation of particles, while viscometry was suitable to monitor changes in the dispersing phase. An analysis of the properties of these systems, mainly in terms of particle–particle and macromolecule–macromolecule interactions was carried out. Copyright © 2004 Society of Chemical Industry 相似文献
3.
4.
Tereza Strkov Jií Lukavský Ondej Javora Cyril Brom 《Journal of Computer Assisted Learning》2019,35(4):555-568
Anthropomorphizing graphical elements in multimedia learning materials improves learning outcomes. The reasons for enhanced learning are unclear. We extended a seminal anthropomorphism study in order to examine whether the effect of anthropomorphisms on learning outcomes, both immediate and delayed, is caused by the anthropomorphized elements' effects on attention distribution or by elevated positive affective–motivational states. The study had a partial 3 × 2 design (the materials' graphics: schematic vs. black‐and‐white anthropomorphisms vs. colourful anthropomorphisms × eye tracker: present vs. absent). The participants were university students (N = 181). Unexpectedly, we found no significant effect of anthropomorphisms on learning outcomes. Anthropomorphisms significantly affected attention distribution during initial fixations but not overall. Modest effect on enjoyment was found, but no such effect was detected as concerns flow and generalized positive affect. We also found that the eye tracker's mere presence had slight adverse effects on learners, but these effects did not compromise learning. 相似文献
5.
Marcos A. Simplicio Bruno T. de Oliveira Cintia B. Margi Paulo S.L.M. Barreto Tereza C.M.B. Carvalho Mats Näslund 《Ad hoc Networks》2013,11(3):1221-1236
Security is an important concern in any modern network. This also applies to Wireless Sensor Networks (WSNs), especially those used in applications that monitor sensitive information (e.g., health care applications). However, the highly constrained nature of sensors imposes a difficult challenge: their reduced availability of memory, processing power and energy hinders the deployment of many modern cryptographic algorithms considered secure. For this reason, the choice of the most memory-, processing- and energy-efficient security solutions is of vital importance in WSNs. To date, a number of extensive analyses comparing different encryption algorithms and key management schemes have been developed, while very little attention has been given to message authentication solutions. In this paper, aiming to close this gap, we identify cipher-based Message Authentication Codes (MACs) and Authenticated Encryption with Associated Data (AEAD) schemes suitable for WSNs and then evaluate their features and performance on a real platform (TelosB). As a result of this analysis, we identify the recommended choices depending on the characteristics of the target network and available hardware. 相似文献
6.
Tereza Kubasova Zuzana Seidlerova Ivan Rychlik 《International journal of molecular sciences》2021,22(11)
In this review, we link ecological adaptations of different gut microbiota members with their potential for use as a new generation of probiotics. Gut microbiota members differ in their adaptations to survival in aerobic environments. Interestingly, there is an inverse relationship between aerobic survival and abundance or potential for prolonged colonization of the intestinal tract. Facultative anaerobes, aerotolerant Lactobacilli and endospore-forming Firmicutes exhibit high fluctuation, and if such bacteria are to be used as probiotics, they must be continuously administered to mimic their permanent supply from the environment. On the other hand, species not expressing any form of aerobic resistance, such as those from phylum Bacteroidetes, commonly represent host-adapted microbiota members characterized by vertical transmission from mothers to offspring, capable of long-term colonization following a single dose administration. To achieve maximal probiotic efficacy, the mode of their administration should thus reflect their natural ecology. 相似文献
7.
J. Vacik V. Hnatowicz J. Cervena H. Naramoto S. Yamamoto D. Fink 《Fullerenes, Nanotubes and Carbon Nanostructures》2001,9(2):197-209
To determinate the stopping cross section in fullerite a feasible approach, taking into account the high radiation sensitivity and mechanical fragility of fullerite films, should be employed. In this work, the stopping cross sections of 1H, 3H, 4He and 7Li ions for several selected energies were measured by Rutherford backscattering, neutron depth profiling and alpha spectroscopy using sandwich structures of samples composed of fullerite deposited on a firm substrate (Si, steel) with an intermediate marker (Au, N, Li, B, Pu). In addition, ion transmission through a thin C film supporting a fullerite layer was also utilized. The measured stopping cross sections follow the theoretical predictions calculated for carbon, but are systematically (10-35%) higher than the theoretical ones (with the exception of 5 — 5.5 MeV 4He). The observed deviation of the experimental data can partly be explained by the chemical state effects in fullerite, which accounts for about 20-50% of the difference. The measured energy straggling exceeds Bohr's value by a factor of about 2 for alpha spectroscopy and ion transmission, and 2.5 or 9.5 for Rutherford backscattering and neutron depth profiling, respectively. The discrepancy can be explained by a thickness variation, such as surface roughness of the fullerite films. 相似文献
8.
Jonathan Chenoweth Julie Barnett Tereza Capelos Chris Fife-Schaw Tanika Kelay 《Water Resources Management》2010,24(15):4339-4358
Models approaching consumer expectations of their water supplier from a risk perspective suggest that consumers primarily
and overwhelmingly want safe drinking water supply. In this study consumer preferences in the water sector are investigated
in two contrasting case studies: Cyprus, where there have been significant quantity and continuity of supply issues, and Riga,
where there have been water quality issues. While water quality is undoubtedly the main priority of water consumers in Riga,
in Cyprus consumers indicate that they prioritise a more reliable service even though many are sufficiently dissatisfied with
water quality that they do not drink the tap water. The analysis of consumer attitudes in the two case studies suggests that
when water supply is unreliable, reliability takes precedence; once it is reliable quality issues come to the fore. 相似文献
9.
Primers designed to amplify a Campylobacter jejuni cadF gene sequence were used in an SYBR Green I real-time PCR assay as an alternative to conventional bacteriological methods for the rapid detection of C. jejuni in foods. Twenty-five grams of chicken skin (breast and thigh) was contaminated by adding approximately 1, 10, or 50 CFU of C. jejuni ATCC 35560. Twenty-five grams of pork and 25-ml aliquots of milk were also inoculated with 1 and 10 CFU of the pathogen. The samples were incubated in Bolton broth for different periods at 37 and 42 degrees C under microaerophilic conditions. Using a commercial robotic DNA purification system, DNA was extracted and purified from 1-ml aliquots of the enrichment cultures before and after centrifugation of the 250-ml enrichment broth at 15,900 x g for 10 min at 4 degrees C. The DNA was used as the template in a real-time PCR assay. C. jejuni was detected after 12 h of enrichment from samples inoculated with about 50 CFU/25-g sample. After centrifugation, an enrichment step of 8 h was sufficient to allow detection of pathogen in samples inoculated with 10 CFU/25 g. However, 24 h of enrichment was necessary to detect pathogen in samples inoculated with approximately 1 CFU/25 g. The real-time PCR protocol developed in this study significantly reduced the detection time of C. jejuni in foods. 相似文献
10.
Tereza Neocleous Colin AitkenGrzegorz Zadora 《Chemometrics and Intelligent Laboratory Systems》2011,109(1):77-85
In forensic science likelihood ratios provide a natural way of computing the value of evidence under competing propositions such as “the compared samples have originated from the same object” (prosecution) and “the compared samples have originated from different objects” (defence). We use a two-level multivariate likelihood ratio model for comparison of forensic glass evidence in the form of elemental composition data under three data transformations: the logratio transformation, a complementary log-log type transformation and a hyperspherical transformation. The performances of the three transformations in the evaluation of evidence are assessed in simulation experiments through use of the proportions of false negatives and false positives. 相似文献