Ratiometric Detection of Glutathione Based on Disulfide Linkage Rupture between a FRET Coumarin Donor and a Rhodamine Acceptor

Abnormal levels of glutathione, a cellular antioxidant, can lead to a variety of diseases. We have constructed a near-infrared ratiometric fluorescent probe to detect glutathione concentrations in biological samples. The probe consists of a coumarin donor, which is connected through a disulfide-tethered linker to a rhodamine acceptor. Under the excitation of the coumarin donor at 405 nm, the probe shows weak visible fluorescence of the coumarin donor at 470 nm and strong near-infrared fluorescence of the rhodamine acceptor at 652 nm due to efficient Forster resonance energy transfer (FRET) from the donor to the acceptor. Glutathione breaks the disulfide bond through reduction, which results in a dramatic increase in coumarin fluorescence and a corresponding decrease in rhodamine fluorescence. The probe possesses excellent cell permeability, biocompatibility, and good ratiometric fluorescence responses to glutathione and cysteine with a self-calibration capability. The probe was utilized to ratiometrically visualize glutathione concentration alterations in HeLa cells and Drosophila melanogaster larvae.     

On the extension of the compositional rule of inference

In this article we introduce an extension of Zadeh's compositional rule of inference in terms of the general rule of inference using a triangular norm extended to n arguments. Using this extension, all inferences schemes, crisp as well as fuzzy, based on the compositional rule of inference can be obtained in a uniform way. © 1993 John Wiley & Sons, Inc.     

Differential Display of Investigative Behavior Permits Discrimination of Scent Signatures from Familiar and Unfamiliar Socially Dominant Female Marmoset Monkeys (Callithrix jacchus)

The hypothesis was tested that marmosets have differential displays of investigatory behavior towards circumgenital scent marks from socially dominant females based on degree of familiarity. In a series of two-choice behavioral discrimination bioassays, dominant males together with subordinate and dominant females were presented concurrently with scent secretions from familiar versus unfamiliar dominant females, and their investigative behaviors towards the scent were recorded. Test animals directed significantly different amounts of investigative behaviors towards familiar versus unfamiliar scents when the scent stimuli involved complete circumgenital scent marks, glandular secretion only, or urine only. When animals discriminated between the two scent samples, they did so by directing significantly increased amounts of behavior to scent from unfamiliar females. Chemical cues promoting differential displays of behavior to familiar versus unfamiliar scents appeared to be volatile in nature and were effective in the scent mark for at least one day following deposition. The reliable ability of marmosets to consistently discriminate between odors from familiar versus unfamiliar dominant females provides strong circumstantial evidence that individual female marmosets have distinct chemical signatures. The ability of marmosets to discriminate the odor from familiar versus unfamiliar dominant females may be important in the context of intergroup relations.     

Filters of residuated lattices and triangle algebras

An important concept in the theory of residuated lattices and other algebraic structures used for formal fuzzy logic, is that of a filter. Filters can be used, amongst others, to define congruence relations. Specific kinds of filters include Boolean filters and prime filters.In this paper, we define several different filters of residuated lattices and triangle algebras and examine their mutual dependencies and connections. Triangle algebras characterize interval-valued residuated lattices.     

Reasoning about fuzzy temporal information from the web: towards retrieval of historical events

When searching for information about historical events, queries are naturally formulated using temporal constraints. However, the structured temporal information needed to support such constraints is usually not available to information retrieval systems. Furthermore, the temporal boundaries of most historical events are inherently ill-defined, calling for suitable extensions of classical temporal reasoning frameworks. In this paper, we propose a framework based on a fuzzification of Allen’s Interval Algebra to cope with these issues. By using simple heuristic techniques to extract temporal information from web documents, we initially focus more on recall than on precision, relying on the subsequent application of a fuzzy temporal reasoner to improve the reliability of the extracted information, and to deal with conflicts that arise because of the vagueness of events. Experimental results indicate that a consistent and reliable knowledge base of fuzzy temporal relations can thus be obtained, which effectively allows us to target temporally constrained retrieval tasks.     

On a reflexivity-preserving family of cardinality-based fuzzy comparison measures

In this paper, we generalize and extend previous work on a triparametric family of cardinality-based fuzzy similarity measures. More specifically, we (i) generalize this family to a broader family of fuzzy comparison measures, (ii) summarize previously obtained results regarding this family and fill up a few gaps by adding some novel results, (iii) compare our parametric family with an already existing one, and (iv) elaborate on the ranking invariance between certain members of the presented triparametric family of cardinality-based fuzzy comparison measures.     

Is cheating a human function? The roles of presence,state hostility,and enjoyment in an unfair video game

In sports and board games, when an opponent cheats, the other players typically greet it with disdain, anger, and disengagement. However, work has yet to fully address the role of the computer cheating in video games. In this study, participants played either a cheating or a non-cheating version of a modified open-source tower-defense game. Results indicate that when a computer competitor cheats, players perceive the opponent as being more human. Cheating also increases player aggravation and presence, but does not affect enjoyment of the experience. Additionally, players that firmly believed that their opponent was controlled by the computer exhibited significantly less state hostility compared to players that were less certain of the nature of their competitor. Game designers can integrate subtle levels of cheating into computer opponents without any real negative responses from the players. The results indicate that minor levels of cheating might also increase player engagement with video games.     

Intuitionistic fuzzy rough sets: at the crossroads of imperfect knowledge

Abstract: Just like rough set theory, fuzzy set theory addresses the topic of dealing with imperfect knowledge. Recent investigations have shown how both theories can be combined into a more flexible, more expressive framework for modelling and processing incomplete information in information systems. At the same time, intuitionistic fuzzy sets have been proposed as an attractive extension of fuzzy sets, enriching the latter with extra features to represent uncertainty (on top of vagueness). Unfortunately, the various tentative definitions of the concept of an ‘intuitionistic fuzzy rough set’ that were raised in their wake are a far cry from the original objectives of rough set theory. We intend to fill an obvious gap by introducing a new definition of intuitionistic fuzzy rough sets, as the most natural generalization of Pawlak's original concept of rough sets.     

Practical implementation of possibilistic probability mass functions

 Probability assessments of events are often linguistic in nature. We model them by means of possibilistic probabilities (a version of Zadeh's fuzzy probabilities with a behavioural interpretation) with a suitable shape for practical implementation (on a computer). Employing the tools of interval analysis and the theory of imprecise probabilities we argue that the verification of coherence for these possibilistic probabilities, the corrections of non-coherent to coherent possibilistic probabilities and their extension to other events and gambles can be performed by finite and exact algorithms. The model can furthermore be transformed into an imprecise first-order model, useful for decision making and statistical inference.     

Interleukin‐4‐Clicked Surfaces Drive M2 Macrophage Polarization

Driving macrophage (M?) polarization into the M2 phenotype provides potential against inflammatory diseases. Interleukin‐4 (IL‐4) promotes polarization into the M2‐M? phenotype, but its systemic use is constrained by dose‐limiting toxicity. Consequently, we developed IL‐4‐decorated surfaces aiming at sustained and localized activity. IL‐4 muteins were generated by genetic code expansion; Lys42 was replaced by unnatural amino acids (uAAs). Both muteins showed cell‐stimulation ability and binding affinity to IL4Rα similar to those of wt‐IL‐4. Copper‐catalyzed (CuAAC) and copper‐free strain‐promoted (SPAAC) 1,3‐dipolar azide–alkyne cycloadditions were used to site‐selectively anchor IL‐4 to agarose surfaces. These surfaces had sustained IL‐4 activity, as demonstrated by TF‐1 cell proliferation and M2, but not M1, polarization of M‐CSF‐generated human M?. The approach provides a blueprint for the engineering of cytokine‐activated surfaces profiled for sustained and spatially controlled activity.