Novel thermally and mechanically stable hydrogel for enzyme immobilization of penicillin G acylase via covalent technique

κ‐Carrageenan hydrogel crosslinked with protonated polyethyleneimine (PEI⁺) and glutaraldehyde (GA) was prepared and evaluated as a novel biocatalytic support for covalent immobilization of penicillin G acylase (PGA). The method of modification of the carrageenan biopolymer is clearly illustrated using a schematic diagram and was verified by FTIR, elemental analysis, DSC, and INSTRON using the compression mode. Results showed that the gels' mechanical strength was greatly enhanced from 3.9 kg/cm² to 16.8 kg/cm² with an outstanding improvement in the gels thermal stability. It was proven that, the control gels were completely dissolved at 35°C, whereas the modified gels remained intact at 90°C. The DSC thermogram revealed a shift in the endothermic band of water from 62 to 93°C showing more gel‐crosslinking. FTIR revealed the presence of the new functionality, aldehydic carbonyl group, at 1710 cm^?1 for covalent PGA immobilization. PGA was successfully immobilized as a model industrial enzyme retaining 71% of its activity. The enzyme loading increased from 2.2 U/g (control gel) to 10 U/g using the covalent technique. The operational stability showed no loss of activity after 20 cycles. The present support could be a good candidate for the immobilization of industrial enzymes rich in amino groups, especially the thermophilic ones. © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2008     

Measurement of Inlet Drop Size Distribution of Secondary Dispersions by Laser Particle Size Analyzer

Knowledge of the Oil drop size distribution, in addition to the mean drop size, is necessary in order to characterize secondary dispersions. Furthermore, the capture efficiency for any drop flowing through a packed bed depends upon its Oil inlet drop diameter. The Oil/Water secondary dispersions produced by the centrifugal pump were analyzed at regular intervals during an experiment to ascertain the drop size distribution and to check that the feed to the coalescence bed was consistent. New techniques that were developed for measurement of drop size distribution of secondary dispersions using Laser Particle Size Analyzer, which consisted of (He/Ne) laser emitter and laser receiver and lenses. The Laser Particle Size Analyzer was fixed directly to the experimental equipment by using the special designed circular cell. The measurement of drop size distribution was done by computer system with application software package. The new mean drop diameter (i.e., d₂₁) equation has been derived theoretically and the results that predicted from this equation exhibited a maximum error of ±15% from the experimental data.     

Processing of an East Mediterranean phosphate ore sample by an integrated attrition scrubbing/classification scheme (part one)

ABSTRACT

In this study, an Egyptian siliceous/calcareous sedimentary phosphorite was subjected to beneficiation by unsophisticated economic means. The mineralogical and chemical examination showed that the ore sample is a typical example for the East Mediterranean phosphorites with 21.80% P₂O₅, 10.93% acid insoluble and 16.61% loss on ignition. The primary crushed sample was classified into three size fractions without any milling and they were separately subjected to an integrated attrition scrubbing study. Screening and classification on the 2.30 mm and 0.071 mm sieves were sequentially introduced with the attrition process. The effect of solid%, attrition speed and time were studied and optimized for each size fraction. Results showed that three products were produced and could be categorized according to their P₂O₅ content into the coarse tailing (+2.30 mm), the attrition concentrate (?2.30 + 0.071 mm) and the phosphate fines (?0.071 mm). The P₂O₅ contents of these products reached 12.24%, 28.72% and 18.77%, with P₂O₅ distribution reached 6.74%, 51.75% and 41.51%, respectively. A global P₂O₅ of 28.72% was obtained with a recovery of about 52%. A tentative flow-sheet for the whole process was postulated.     

Effects of refining on chemical and physical properties of palm oil products

Some chemical changes in the composition and physical properties of palm oil products are discussed. The effects of bleaching and deodorization on oxidative properties and possible isomerization and interesterification of the fatty acids were indicated from laboratory refining experiments. Investigation of commercial samples of refined palm oil products showed that the conjugated dienes and trienes formed are minimal, indicating the use of good quality raw materials and mild processing conditions. Very little isomerization occurred in commercial refined products as indicated from the level oftrans acids, and changes in the POP to PPO triglycerides due to possible interesterification were insignificant. Changes in physical properties were inevitable due to the removal of free fatty acids and diglycerides and to minor impurities.     

A rule‐based approach to detect and prevent inconsistency in the domain‐engineering process

A medium‐sized domain‐engineering process can contain thousands of features that all have constraint dependency rules between them. Therefore, the validation of the content of domain‐engineering process is vital to produce high‐quality software products. However, it is not feasible to do this manually. This paper aims to improve the quality of the software products generated by the domain‐engineering process by ensuring the validity of the results of that process. We propose rules for two operations: inconsistency detection and inconsistency prevention. We introduce first‐order logic (FOL) rules to detect three types of inconsistency and prevent the direct inconsistency in the domain‐engineering process. Developing FOL rules to detect and prevent inconsistency in the domain‐engineering process directly without the need to the configuration process is our main contribution. We performed some experiments to test the scalability and applicability of our approach on domain‐engineered software product lines containing 1000 assets to 20000 assets. The results show that our approach is scalable and could be utilized to improve the domain‐engineering process.     

Compact broadband planar orthomode transducer

The design and test results of a compact C-band orthomode transducer are presented. The transducer comprises four rectangular probes orthogonally arranged in a circular waveguide, designed to work in the WG13 band. Measurements of the system in the frequency range 4.64 - 7.05 GHz agree very well with simulation results and show a cross-polarisation level below -58 dB, a return loss of about -20 dB, and an insertion loss difference of less than 0.18 dB between the orthogonal polarisation modes across the full waveguide band.     

Investigation of microwave propagation in high-temperaturesuperconducting waveguides

It is well known that the boundary conditions of the electromagnetic fields on the surface of a superconductor are influenced by the field penetration into the material. In a series of recent publications, it has been suggested that this effect substantially influences the wave propagation in high-temperature superconducting waveguides, to the extent that the mode order becomes different than that predicted for perfect conductor waveguides. In this paper, we present experimental investigation of this effect. We show that the effect of superconductivity on the wave propagation in waveguides is very small for temperatures well below the transition temperature and away from cutoff. We also discuss the behavior of the waveguide near cutoff and very close to the transition temperature     

Dyadic analysis of partially coherent submillimeter-wave antennasystems

We describe a procedure for simulating the behavior of partially coherent submillimeter-wave antenna systems. The procedure is based on the principle that the second-order statistical properties of any partially coherent vector field can be decomposed into a sum of fully coherent, but completely uncorrelated, natural modes. Any of the standard electromagnetic analysis techniques-physical optics, geometrical theory of diffraction, etc.-can be used to propagate and scatter the modes individually, and the statistical properties of the total transformed field reconstructed at the output surface by means of superposition. In the case of modal optics-plane waves, Gaussian optics, waveguide mode matching, etc.-the properties of the field can be traced directly by means of scattering matrices. The overall procedure is of considerable value for calculating the behavior of astronomical instruments comprising planar and waveguide multimode bolometers, submillimeter-wave optical components, and large reflecting antennas     

Unusual nasal foreign body detected on routine dental radiography: case report

This report describes an unusual nasal foreign body in a 4-year-old boy discovered incidentally in a routine dental radiograph. A small piece of rubber eraser was lodged in the right nasal cavity, causing unilateral nasal obstruction and discharge, sneezing, snoring and breathing difficulty. In this case, discovery of unknown intranasal object was diagnostic for a condition suspected of pathological origin. Dentists may play a significant role in the diagnosis of intranasal foreign objects in children, through careful clinical examination and interpretation of dental radiographs. Early diagnosis is emphasized in order to avoid complications.     

Physical Properties of Aqueous N-Methyl Pyrrolidone at Different Temperatures

Many physical properties of aqueous N-Methyl pyrrolidone mixtures were determined for molar fraction X_NMP = 0.02, 0.05, 0.125, 0.32, 0.37 at different temperatures ranging from 15-65°C. From the result we noticed that the viscosity coefficient was increased with increasing the molar fraction X_NMP and reached a maximum value at X_NMP = 0.25-0.37, and decreased with temperature. We observed the same relationship of the thermodynamic properties for the mixture at different temperatures and for excess activation function was positive. They reached maximum value at the X_NMP = 0.25-0.37. The same relationship was observed for excess dielectric constant. The results were explained according to hydrogen bonding effects and dipole-dipole interactions. This binding is stronger than binding between water molecules which may be let to form a complex at the molal fraction ratio 2:1 of water-NMP solution. We noticed that an increase in temperature may be let to tighten the interaction between water and NMP.