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1.
The role of gut intraepithelial lymphocytes (IEL) in immunity to cryptosporidial infection was investigated with a murine infection model involving Cryptosporidium muris. Oocyst shedding was monitored in severe combined immunodeficiency (SCID) mice infected with C. muris following intravenous injection of mesenteric lymph node (MLN) cells or intestinal IEL from BALB/c donor mice which were naive or previously infected with C. muris. SCID mice receiving no lymphoid cells developed chronic infections and excreted large numbers of oocysts until the end of the experiment. SCID mice injected with IEL from immune animals, however, were able to overcome the infection, and furthermore, these animals produced fewer oocysts and recovered sooner than ones which received IEL or MLN cells from naive BALB/c donors. Similar levels of protection were obtained in SCID mice injected with either 2 X 10(6) IEL or MLN cells from immune donor mice. Depletion of CD4+ cells from immune IEL, however, abrogated the ability to transfer immunity to SCID mice, while depletion of CD8+ cells only marginally reduced the protective capacity of immune IEL. Finally, control SCID mice which received no lymphocytes had < or = 1% CD4+ cells in the IEL from the small intestine, whereas the IEL from SCID mice recovered from infection, as a result of injection with immune IEL, contained 15% CD4+ cells. Thus, the ability to control C. muris infection correlated with the presence of the protective CD4+ cells in the gut epithelium. 相似文献
2.
Suominen Fuller M.L. Rodriguez Fernandez L. Massoumi G.R. Lennard W.N. Kasrai M. Bancroft G.M. 《Tribology Letters》2000,8(4):187-192
X‐ray absorption near edge structure (XANES) spectroscopy at the P K‐edge was used to monitor ZDDP antiwear film thickness
with rubbing time. Thermal immersion films of varying thickness were generated from the ZDDP and analysed using XANES spectroscopy
and the particle induced X‐ray emission (PIXE) technique. P K‐edge XANES edge jumps and (1s → np) peak heights of the spectra
were plotted against PIXE mass thickness values in order to establish calibration curves. Antiwear films were analysed using
XANES spectroscopy, and average mass thicknesses were extrapolated from the calibration curves. A set of antiwear films formed
in the presence of ZDDP and then further rubbed in base oil (no ZDDP) showed no significant decrease in film thickness. A
set of antiwear films rubbed in the presence of ZDDP for various lengths of time showed an increase in film thickness, followed
by thinning of the film. The decrease in film thickness is believed to be due to wear caused by the ZDDP solution decomposition
products acting as an abrasive in the contact region.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
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M.?A.?Nicholls P.?R.?NortonEmail author G.?M.?Bancroft M.?Kasrai G.?De.?Stasio L.?M.?Wiese 《Tribology Letters》2005,18(3):261-278
summary Understanding the lubrication of aluminum–silicon (Al–Si) alloys (>18 Si) under conditions similar to those in the cylinder/bore system is vital to determining their applicability to current engine designs. A novel investigation of the location of zinc-dialkyl-dithiophosphate (ZDDPs) antiwear (AW) film formation on an Al–Si alloy has been performed using X-ray absorption near edge structure (XANES) analysis, X-ray photoelectron emission spectroscopy (X-PEEM), and imaging nanoindentation techniques. A study of the initial stages of wear (10 min) to prolonged rubbing (60 min) was performed. The findings show that the film forms primarily on the raised silicon grains and is consistent with a zinc polyphosphate glass. The film has an elastic modulus of ~70 GPa and a similar elastic response to a ZDDP AW film formed on steel under the same conditions. This provides the first direct observation and characterization of a ZDDP antiwear film on Al–Si alloys using spatially resolved chemical and mechanical techniques at the nanoscale. 相似文献
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Recent studies have demonstrated the feasibility of using DNA-based experiments to compute solutions to combinatorial problems. However, a prerequisite for designing a computer useful in a wide range of applications is the ability to perform mathematical calculations. The development of a DNA-based algorithm for addition is presented. The DNA representation of two nonnegative binary numbers is presented in a form permitting a chain of primer extension reactions to carry out the addition operation. To demonstrate the feasibility of this algorithm, a simple example was executed biochemically. 相似文献
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In 5 single-S experiments, depressed mood and corrugator EMG were measured while 5 female depressed psychiatric hospital day-clinic patients thought happy and unhappy thoughts. Results show that mood was significantly more depressed after unhappy thoughts for all Ss. During the 2nd half of the thought periods, corrugator EMG was higher in the unhappy condition than in the happy condition for all Ss, significantly so for all but one. Significant positive correlations between depressed mood and corrugator EMG were obtained in all Ss. Findings are discussed in relation to cognitive theories of depression and to the usefulness of corrugator EMG as an index of depressed mood. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献