Dual needle corona discharge to generate stable bipolar ion for neutralizing electrosprayed nanoparticles

The performances of dual needle corona discharge (NCD) as bipolar ion source to neutralize the electrospray (ES) particles were characterized and optimized. The NCD was constructed from a tungsten needle and grounded mesh electrode in the needle-to-plane configuration. The dual NCD created a bipolar ion environment by mixing the balanced concentration of positive and negative ions produced by each NCD. The dual NCD was placed in an electrospray aerosol generator (EAG) apparatus to reduce the charge state of the ES particles. Polystyrene latex (PSL) suspensions having the particle size range of 96–256 nm were used as the precursor solution for the electrospray process. Some characterizations to the NCD were carried out to obtain optimum operating voltage and air flow rate. The size distribution and charge fraction of the electrospray PSL (ES-PSL) particles exiting the EAG were also investigated. The result showed the dual NCD could generate stable bipolar ions by mixing positive and negative ions with balanced concentration. The bipolar ions from the dual NCD were capable of neutralizing and reducing the charge state of highly charged ES-PSL particles larger than 100 nm. The EAG, equipped with the dual NCD, could generate ES-PSL particles with stable concentration and consistent size distribution. The charge fraction calculation of the ES-PSL particles showed that more than 80% of the particles exiting the EAG were positively charged.     

Development of a new personal air filter test system using a low-cost particulate matter (PM) sensor

Abstract

The extensive use of air filters has encouraged advances in both the fabrication and characterization of air filter technology. An affordable and accessible means of assessing the quality of air filters is greatly needed because of the high demand for these filters. We developed a personal air filter test (PAFT) system for measuring filter pressure drop, efficiency, and quality of filtration. The PAFT system utilizes a PM sensor (Sharp, GP2Y1010AU0F) to measure filtration efficiency. PM sensor performance was evaluated and optimized to guarantee its suitability for this application. The sensor performance evaluation studied the output responses to sampling flow, particle diameter, and PM sources. We also improved the sensor’s sensitivity. The experimental results show that the sensor had no significant influence on the sampling flow. The sensor output was highly dependent on the particle size and PM source, but its response curves remained linear, which was an advantage for filter efficiency measurement. We measured the efficiency of nanofiber filters of various efficiencies, comparing the results to a reference efficiency as measured by a CPC (TSI, 3772). The test resulted in a filtration coefficient (K_f), which was used to correct the PAFT efficiency measurement results. We also conducted filtration efficiency tests on commercial mask filters and the results showed good agreement to the reference, with a small average error of about 2.5%. The complete design of the PAFT and experimental methods is discussed in detail.

Copyright © 2020 American Association for Aerosol Research     

Proliferation study and microscopy evaluation on the effects of tannic acid in human fetal osteoblast cell line (hFOB 1.19)

Tannic acid (TA) is a phenolic compound that might act directly on osteoblast metabolism. The study was performed to investigate the effects of TA on the proliferation, mineralization, and morphology of human fetal osteoblast cells (hFOB 1.19). The cells were divided into TA‐treated, untreated, and pamidronate‐treated (control drug) groups. Half maximal effective concentration (EC₅₀) values for TA and pamidronate were measured using MTT assay. The EC₅₀ of hFOB 1.19 cells treated with TA was 2.94 M. This concentration was more effective compared to the pamidronate (15.27 M). Cell proliferation assay was performed to compare cell viability from Day 1 until Day 14. The morphology of hFOB 1.19 was observed via inverted microscope and scanning electron microscope. Calcium (Ca) and phosphate (P) were assessed using energy‐dispersive X‐ray (EDX) analysis. Furthermore, the mineralization of hFOB 1.19 was determined by von Kossa staining (P depositions) and Alizarin Red S staining (Ca depositions). The number of cells treated with TA was significantly higher than the two control groups at Day 10 and Day 14. The morphology of cells treated with TA was uniformly fusiform‐shaped with filopodia extensions. Besides, globular‐like structures of deposited minerals were observed in the TA‐treated group. In line with other findings, EDX spectrum analysis confirmed the presence of Ca and P. The cells treated with TA had significantly higher percentage of both minerals at Day 3 and Day 10 compared to the two control groups. In conclusion, TA enhances cell proliferation and causes cell morphology changes, as well as improved mineralization.