Intrinsic Fluorescence of the Active and the Inactive Functional Forms of Human Thymidylate Synthase

The observables associated with protein intrinsic fluorescence – spectra, time decays, anisotropies – offer opportunities to monitor in real time and non-invasively a protein‘s functional form and its interchange with other forms with different functions. We employed these observables to sketch the fluorometric profiles of two functional forms of human thymidylate synthase (hTS), a homodimeric enzyme crucial for cell proliferation and thus targeted by anticancer drugs. The protein takes an active and an inactive form. Stabilization of the latter by peptides that, unlike classical hTS inhibitors, bind it at the monomer/monomer interface offers an alternative inhibition mechanism that promises to avoid the onset of drug resistance in anticancer therapy. The fluorescence features depicted herein can be used as tools to identify and quantify each of the two protein forms in solution, thus making it possible to investigate the kinetic and thermodynamic aspects of the active/inactive conformational interchange. Two examples of fluorometrically monitored interconversion kinetics are provided.     

Generation of bioenergy and biofertilizer on a sustainable rural property

Energy sources have become a focus of legal, ethical, social and economic pressures due to increasing environmental problems. Nonrenewable energy sources are being increasingly replaced by other sources that are renewable and less pollutive, with technologies aimed at sustainability. Companies that adopt alternative forms of energy will be able to increase their competitiveness and use wastes to generate energy, which is an ecological and economical efficiency approach. In the interior of the state of São Paulo, Brazil, a rural producer of pork crackling and coffee evaluated the entire production cycle of his products and wastes, seeking to achieve energy self sufficiency and identify new possibilities for gains through cost reductions. Biodiesel, soap and detergent are produced with the waste animal fat left from the production of pork crackling. Pig farm wastes become biomass for a biodigester, which produces sufficient biogas to keep the piglets warm, dry the coffee, fry the pork crackling, and generate electricity, among other uses. The biofertilizer produced by the biodigester helps fertilize coffee and corn plantations. The purpose of this article is to demonstrate the ecological and economic feasibility of the project. The research method used here is a case study involving qualitative analyses of environmental and social variables and quantitative analyses of economic variables. It is concluded that the investment made in this project yields excellent returns, with savings of 100% in fuels, 50% in electric energy and 40% in fertilizer, in addition to gains from the products developed with the wastes.     

Novel Heterogenized Palladium(II) Complexes Anchored to Polymer Matrices Functionalized with Bis(diphenylphosphino)methane Moieties

The synthesis and structural characterization of a novel bidentate ligand such as bis(diphenylphosphino) methane bound to a cross-linked styrene/divinylbenzene resin and to linear poly(styrene) are reported. Moreover, the anchoring of palladium acetate to the above polymeric ligands is described and the structure of the heterogenized palladium(II) complexes obtained is proposed.     

Ortho‐ vs Bay‐Functionalization: A Comparative Study on Tetracyano‐Terrylenediimides

In this paper n‐type semiconductors synthesized via selective fourfold cyanation of the ortho‐ and bay‐positions (2,5,10,13‐ and 1,6,9,14‐positions respectively) of teyrrylenediimides are reported. A detailed study about the impact of the diverse functionalization topologies on the optoelectronic properties, self‐organization from solution, solid‐state packing, and charge carrier transport in field‐effect transistors is presented. The ortho‐substitution preserves the planarity of the core and favors high order in solution processed films. However, the strong intermolecular interactions lead to a microstructure with large aggregates and pronounced grain boundaries which lower the charge carrier transport in transistors. In contrast, the well‐soluble bay‐functionalized terrylenediimide forms only disordered films which surprisingly result in n‐type average mobilities of 0.17 cm²/Vs after drop‐casting with similar values in air. Processing by solvent vapor diffusion enhances the transport to 0.65 cm²/Vs by slight improvement of the order and surface arrangement of the molecules. This mobility is comparable to highest n‐type conductivities measured for solution processed PDI derivatives demonstrating the high potential of TDI‐based semiconductors.     

Flavonoids as stabilizers of fish oil: An alternative to synthetic antioxidants

The antioxidant activities against fish oil oxidation of six commercially available flavonoids and of five flavonoids purified from two Chilean native plants were compared to those ofdl-α-tocopherol and of two synthetic antioxidants, butylated hydroxytoluene and butylated hydroxyanisole. Among the commercial flavonoids, catechin, morin and quercetin showed a higher activity when fish oil oxidation (either spontaneous or Fe²⁺-induced) was assessed from the formation of peroxides or thiobarbituric acid-reactive substances. Among the native flavonoids, the 5,3′,4′-trihydroxy-7-methoxy flavanone (designated as Pt-2) showed the highest antioxidant activity. Mixtures of quercetin or of Pt-2 withdl-α-tocopherol produced better inhibitory effects when compared to that of each substance assayed by itself. Also, when Pt-2 and quercetin were assayed in combination (0.3 g/kg oil and 0.7 g/kg oil, respectively), a synergistic antioxidant effect was observed. Results indicate that several flavonoids could be used as natural antioxidants as a means to replace those synthetic antioxidants, the use of which has been questioned.     

Simultaneous optical signal-to-noise ratio and differential group delay monitoring based on degree of polarization measurements in optical communications systems

We present a simultaneous optical signal-to-noise ratio (OSNR) and differential group delay (DGD) monitoring method based on degree of polarization (DOP) measurements in optical communications systems. For the first time in the literature (to our best knowledge), the proposed scheme is demonstrated to be able to independently and simultaneously extract OSNR and DGD values from the DOP measurements. This is possible because the OSNR is related to maximum DOP, while DGD is related to the ratio between the maximum and minimum values of DOP. We experimentally measured OSNR and DGD in the ranges from 10 to 30 dB and 0 to 90 ps for a 10 Gb/s non-return-to-zero signal. A theoretical analysis of DOP accuracy needed to measure low values of DGD and high OSNRs is carried out, showing that current polarimeter technology is capable of yielding an OSNR measurement within 1 dB accuracy, for OSNR values up to 34 dB, while DGD error is limited to 1.5% for DGD values above 10 ps. For the first time to our knowledge, the technique was demonstrated to accurately measure first-order polarization mode dispersion (PMD) in the presence of a high value of second-order PMD (as high as 2071 ps(2)).     

Temperature Control of a Thermal Plasma Torch with Inductive Coupling Using the Arduino Board

Plasma torch is a device that transforms electrical energy into heat carried by a gas and its safe operation is necessary to control her temperature. This paper presents the use of the Arduino board in temperature control of a plasma torch through fuzzy control. The plasma torch of this project was built so that a flow of water can circulate through your body, allowing its cooling. The cooling system mounted consists of one radiator, one expansion vase, one water pump and one temperature sensor. The heated water coming the plasma torch is passed by the temperature sensor. This is converted in a voltage and read by an analog input port of the Arduino. This processes the information received and makes the decision to turn on/off the radiator fan and/or powered the frequency inverter water pump to control the temperature. The graph of the fuzzy control showed an oscillation between 104 °F to 122 °F around the chosen reference 113 °F. The results show that it is possible to control the temperature of a plasma torch using the Arduino board and fuzzy logic.     

A methodology to assess the availability of next-generation data centers

The Journal of Supercomputing - Cloud data center providers benefit from software-defined infrastructure once it promotes flexibility, automation, and scalability. The new paradigm of...     

Determining relevance of imprecise temporal intervals for cultural heritage information retrieval

Time is an essential concept in cultural heritage applications. Instances of temporal concepts such as time intervals are used for the annotation of cultural objects and also for querying datasets containing information about these objects. Hence it is important to match query and annotation intervals by examining their similarity or closeness. One of the problems is that in many cases time intervals are imprecise. For example, the boundaries of the “Pre-Roman age” and the “Roman age” are inherently imprecise and it may be difficult to distinguish them with clear-cut intervals. In this paper we apply the fuzzy set theory to model imprecise time intervals in order to determine relevance of the relationship between two time intervals. We present a method for matching query and annotation intervals based on their weighted mutual overlapping and closeness. We present (1) methods for calculating these weights to produce a combined measure and (2) results of comparing the combined measure with human evaluators as a case study. The case study takes into consideration archaeological temporal information, which is in most cases inherently fuzzy, and therefore offers a particularly complex and challenging scenario. The results show that our new combined measure that utilizes different weighted measures together in rankings, performs the best in terms of precision and recall. It should be used when ranking annotation intervals according to a given query interval in cultural heritage information retrieval. Our approach intends to be generalizable: overlapping and closeness may be calculated between any two fuzzy temporal intervals. The presented procedure of using user evaluation results as a basis for assigning weights for overlapping and closeness could potentially be used to reveal weights in other domains and purposes as well.