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1.
Peter Eaton Andrea Ragusa Caroline Clavel Cristina T Rojas Paul Graham Raúl V Durán Soledad Penadés 《IEEE transactions on nanobioscience》2007,6(4):309-318
Glyconanoparticles which present carbohydrate and amino groups motifs at their surface were produced. These particles were highly stable and soluble in aqueous solutions. The presence of the carbohydrate groups also allowed the inclusion of more strongly binding groups, without affecting solubility. The binding of a model DNA, plasmid by these nanoparticles was studied by atomic force microscopy, transmission electron microscopy, and gel electrophoresis. Significant differences between the nanoparticles based on their affinities for the DNA were found, with implications for their potential use as nonviral gene delivery agents. 相似文献
2.
The fluctuations observed by recording the picosecond pulses of a mode-locked laser with a synchroscan streak camera are due to the laser jitter and to the variations of the zero crossing of the synchronization. By means of a simple model, it is shown that the dispersions measured when the synchronization is generated from the mode-locker oscillator reflect the instantaneous time phase fluctuations of this oscillator. From the study of the experimental recordings with methods of time series analysis it results that the phase noise of the electronic oscillator is consistent with an underlying low-dimensional strange attractor. 相似文献
3.
Seven hundred three members of the Society for Industrial and Organizational Psychology indicated agreement or disagreement with 49 propositions regarding cognitive ability tests in organizations. There was consensus that cognitive ability tests are valid and fair, that they provide good but incomplete measures, that different abilities are necessary for different jobs, and that diversity is valuable. Items dealing with the unique status of cognitive ability were most likely to generate polarized opinions. A 2-factor model, classifying items as those reflecting societal concerns over the consequences of ability testing and those reflecting an emphasis on the unique status of "g," fit the data well, and these factors proved especially important for predicting responses to the more controversial items. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
4.
Tong B. Tang M. M. Chaudhri Caroline S. Rees S. J. Mullock 《Journal of Materials Science》1987,22(3):1037-1044
Experimental investigations have been made in which single crystals of solid explosives such as cyclotrimethylene trinitramine, RDX, pentaerythritol tetranitrate, PETN, AgN3 and Pb(N3)2, were irradiated with a focused laser beam of wavelength 266 nm and duration 5 nsec in an ultra high vacuum system housing a fast and sensitive time-of-flight mass spectrometer. From the mass spectra (both +ve and -ve ions) of the chemical species obtained, possible decomposition schemes of RDX and PETN have been proposed. For silver and lead azides, the laser irradiation did not cause explosions if the power density was sufficiently low, but micrometre size craters were formed. In the mass spectra of AgN3 products N3
– and silver cluster ions, Ag
n
+, have been found for the first time. It has been suggested that such investigations may possibly throw some light on our understanding of the reactions taking place in a deflagration/detonation wave. 相似文献
5.
Caroline K. Brennan Marya Y. Ornelas Zi W. Yao Prof. Dr. Jennifer A. Prescher 《Chembiochem : a European journal of chemical biology》2021,22(16):2650-2654
Bioluminescent tools have been used for decades to image processes in complex tissues and preclinical models. However, few distinct probes are available to probe multicellular interactions. We and others are addressing this limitation by engineering new luciferases that can selectively process synthetic luciferin analogues. In this work, we explored naphthylamino luciferins as orthogonal bioluminescent probes. Three analogues were prepared using an optimized synthetic route. The luciferins were found to be robust emitters with native luciferase in vitro and in cellulo. We further screened the analogues against libraries of luciferase mutants to identify unique enzyme-substrate pairs. The new probes can be used in conjunction with existing bioluminescent tools for multi-component imaging. 相似文献
6.
The iridium-silicon phase diagram on the silicon-rich side was investigated by means of X-ray powder diffraction, density, differential thermal analysis (DTA), metallography, microprobe analysis, and electrical resistivity. Attempts were made to prepare eight previously reported silicon-rich iridium silicide compounds by arc melting and Bridgman-like growth. However, microprobe analysis identified only five distinct compositions: IrSi, Ir4Si5, Ir3Si4, Ir3Si5 and IrSi≈3. The existence of Ir2Si3, Ir4Si7, and IrSi2 could not be confirmed in this study. DTA in conjunction with X-ray powder diffraction confirm polymorphism in IrSi≈3, determined to have orthorhombic and monoclinic unit cells in the high and low temperature forms. A eutectic composition alloy of 80.5 ± 1 at.% Si was observed between IrSi≈3 and silicon. Both Ir4Si5 and Ir3Si4 exhibit distinct metallic behavior while Ir3Si5 is semiconducting. IrSi and IrSi≈3 exhibit nearly temperature-independent electrical resistivities on the order of (5–10) × 10−6 ω m. 相似文献
7.
Lipid products formed during desaturation of [1-14C] stearyl CoA by hen liver microsomes 总被引:1,自引:0,他引:1
A number of lipid products are formed during the desaturation of stearyl CoA by hen liver microsomes. This article presents an analysis of the products formed when [1-14C] stearyl CoA is incubated with hen liver microsomes for various time periods. [1-14C] Oleyl CoA was the first radioactive unsaturated product formed. Synthesis of phospholipids containing [1-14C] oleic acid occurs only after the desaturase activity has begun to decline. The specific radioactivity of [1-14C] oleyl CoA was similar to the specific radioactivity of [1-14C] stearyl CoA at all time periods tested. The specific radioactivities of [1-14C] oleic acid and phospholipids containing [1-14C] oleic acid were much lower than that of the [1-14C] stearyl CoA. 相似文献
8.
9.
Caroline Fauquant Valrie Briard Nadine Leconte Marie‐Caroline Michalski 《European Journal of Lipid Science and Technology》2005,107(2):80-86
The aim of this study was to characterize the fatty acid composition of the core and membrane of differently sized milk fat globules separated by microfiltration, which can now be used to manufacture dairy products. Native milk fat globules of various mean diameters, ranging from d43 = 2.3 µm to 8.0 µm, were obtained using microfiltration of raw whole milk. After milk fat globule washing, the milk fat globule membrane (MFGM) and the triglyceride core (TC) were separated by manual churning. After total lipid extraction from each fraction, their fatty acid composition was characterized using methyl ester analysis by gas chromatography. Regardless of season, no significant differences were observed in the fatty acid composition of the MFGM phospholipids. Conversely, significant differences were found in the fatty acid composition of TC; particularly, small fat globule TC contained more medium‐chain fatty acids and less stearic acid than large fat globule TC. These results show that the previously observed differences in total fatty acid composition among differently sized milk fat globules are due to their triglyceride composition and MFGM amount rather than to the composition of the MFGM. 相似文献
10.
The action of the tumor promoter, phorbol 12,13-dibutyrate (PDBu), on rabbit peritoneal and human neutrophils is associated
with stimulation of14C-arachidonic acid incorporation into phospholipids within 1–2 min. Stimulated14C-arachidonate incorporation was relatively selective for phosphatidylinositol (PI) in rabbit neutrophils. In contrast, the
secretory response of human neutrophils to PDBu coincided with stimulated label incorporation into phosphatidylserine (PS),
phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid (PA) and PI. Significant increases in label incorporation
were observed with PDBu concentrations as low as 2 nM, and the dose response of stimulated label incorporation paralleled
that of evoked lysozyme secretion. A parallel, but partial, inhibition of PDBu-stimulated PI labeling and enzyme release was
observed after exposing rabbit neutrophils to calcium-deprived medium, whereas calcium deprivation failed to significantly
depress either of these stimulant actions of PDBu in human neutrophils. Further, in rabbit neutrophils PDBu elicited an increase
in cell associated45Ca. However, PDBu was unable to promote the incorporation of32P orthophosphate into PI or enhance phospholipase A2 activity in broken cells. These findings suggest that one expression of the interaction between phorbol esters and their
receptors on neutrophils involves the turnover of arachidonic acid in phospholipids. This stimulated turnover of arachidonate
may be a critical step in the cascade of events associated with neutrophil activation. 相似文献