Chronically Elevated Exogenous Glucose Elicits Antipodal Effects on the Proteome Signature of Differentiating Human iPSC-Derived Pancreatic Progenitors

The past decade revealed that cell identity changes, such as dedifferentiation or transdifferentiation, accompany the insulin-producing β-cell decay in most diabetes conditions. Mapping and controlling the mechanisms governing these processes is, thus, extremely valuable for managing the disease progression. Extracellular glucose is known to influence cell identity by impacting the redox balance. Here, we use global proteomics and pathway analysis to map the response of differentiating human pancreatic progenitors to chronically increased in vitro glucose levels. We show that exogenous high glucose levels impact different protein subsets in a concentration-dependent manner. In contrast, regardless of concentration, glucose elicits an antipodal effect on the proteome landscape, inducing both beneficial and detrimental changes in regard to achieving the desired islet cell fingerprint. Furthermore, we identified that only a subgroup of these effects and pathways are regulated by changes in redox balance. Our study highlights a complex effect of exogenous glucose on differentiating pancreas progenitors characterized by a distinct proteome signature.     

Investigation of the Anti-Methicillin-Resistant Staphylococcus aureus Activity of (+)-Tanikolide- and (+)-Malyngolide-Based Analogues Prepared by Asymmetric Synthesis

Herein, we report antibacterial and antifungal evaluation of a series of previously prepared (+)-tanikolide analogues. One analogue, (4S,6S)-4-methyltanikolide, displayed promising anti-methicillin-resistant Staphylococcus aureus activity with a MIC of 12.5 µg/mL. Based on the antimicrobial properties of the structurally related (−)-malyngolide, two further analogues (4S,6S)-4-methylmalyngolide and (4R,6S)-4-methylmalyngolide bearing a shortened n-nonyl alkyl side chain were prepared in the present study using a ZrCl₄-catalysed deprotection/cyclisation as the key step in their asymmetric synthesis. When these were tested for activity against anti-methicillin-resistant Staphylococcus aureus, the MIC increased to 50 µg/mL.     

The biodrying process of sewage sludge – a review

Abstract

Sewage treatment can be classified into three phases: liquid, gaseous, and solid. Treatment of solids is performed in several steps, and the greatest difficulty in removing water from sewage sludge is due to the bound water present in the sludge. Thus, biodrying, a drying method that can be classified as biological, has been recently applied to treat this type of waste. This review paper aims to report and compile data from 49 biodrying assays of sewage sludge found in the literature (through the present, 2019) into one synthesis table, to discuss and compare the numerous variables found in these papers and their implications for biodrying, and to suggest possibilities for future research. This paper additionally intends to improve knowledge of biodrying and to consequently contribute to the monitoring and understanding of the process.     

Variability for oil and fatty acid composition in castorbean varieties

Thirty-six castorbean varieties were surveyed for oil and fatty acid composition, in order to determine variability of these seed compounds. A large variability of seed oil percentage was observed, ranging from 39.6 to 59.5%. Concerning the fatty acids, little variability was observed for ricinoleic acid, which was the most abundant in the oil, ranging from 83.65 to 90.00%. The other fatty acids appeared in small concentrations and showed a small range: 0.87 to 2.35, 0.68 to 1.84, 2.96 to 5.64, 3.19 to 5.98, and 0.34 to 0.91%, for palmitic, stearic, oleic, linoleic, and linolenic acid, respectively. Non-significant correlations were observed between fatty acids and seed oil percentage. However, significant correlations were observed among fatty acid concentrations: positive and negative ones. These significant correlations could be associated with the biosynthetic pathways of the fatty acids, which are not fully elucidated. They suggest, however, that selection for a particular fatty acid will tend to increase those positively correlated, and decrease those negative ones. Selection and plant breeding techniques could then be applied to modify the oil content of the castorbean seeds, considering the variability observed. For the fatty acid composition, however, the variability was not large enough to make substantial changes in their concentrations by selection procedures. More varieties should be surveyed to find out if such variability is available.     

TRIMS,a privacy-aware trust and reputation model for identity management systems

Electronic transactions are becoming more important everyday. Several tasks like buying goods, booking flights or hotel rooms, or paying for streaming a movie, for instance, can be carried out through the Internet. Nevertheless, they are still some drawbacks due to security threats while performing such operations. Trust and reputation management rises as a novel way of solving some of those problems. In this paper we present our work TRIMS (a privacy-aware trust and reputation model for identity management systems), which applies a trust and reputation model to guarantee an acceptable level of security when deciding if a different domain might be considered reliable when receiving certain sensitive user’s attributes. Specifically, we will address the problems which surfaces when a domain needs to decide whether to exchange some information with another possibly unknown domain to effectively provide a service to one of its users. This decision will be determined by the trust deposited in the targeting domain. As far as we know, our proposal is one of the first approaches dealing with trust and reputation management in a multi-domain scenario. Finally, the performed experiments have demonstrated the robustness and accuracy of our model in a wide variety of scenarios.     

Preparation of polypropylene‐based nanocomposites using nanosized MCM‐41 as support and in situ polymerization

MCM‐41 nanoparticles were used for preparing nanocomposites through the in situ polymerization of propylene. The performance of the catalytic system and the final properties of the materials obtained are highly dependent on the methodology used for impregnation of the catalyst onto the support particles, and therefore an optimization study for the impregnation methodology of the catalyst (Me₂Si(Ind)₂ZrCl₂) was carried out. Two different methodologies were used; the results in terms of catalytic activity and polymer molecular masses indicated that the most promising one involved the pre‐activation of the catalyst with the cocatalyst, methylaluminoxane, followed by impregnation onto the MCM‐41 nanoparticles. Thus, an optimized route for the preparation of polypropylene nanocomposites achieving significant improvements in catalyst activity was developed. The nanocomposite materials were characterized by GPC, TGA and DSC. The dispersion state and the size of the nanoparticles incorporated in the polypropylene matrix were investigated by transmission electron microcopy. Additionally, this methodology allows simultaneous control of the desired amount of support and the concentration of catalyst to be used in the in situ polymerization. © 2015 Society of Chemical Industry     

The ALTER-NATIVA knowledge management approach

Nowadays, it is commonly known that information systems need an agile capability of handling knowledge. To accomplish this, systems have to have a formal knowledge representation ability supported by specific and advanced reasoning features. This research work proposes a knowledge management approach with the purpose to gather, model and consume community knowledge for specific recommendation commitments. Such approach is accomplished by a semantic lexicon alignment between the various community knowledge assets, to facilitate collaborations establishment between people and systems in an interoperable fashion. Thus, a knowledge base supported by a thesaurus able to represent all the metadata needed to represent and characterize the various community stakeholders’ resources is proposed. The thesaurus represents the lexicon in the domain, which in the ALTER-NATIVA systems is mostly used to support the various e-Learning elements (e.g. courses) and users categorization, sustained by synchronization features to facilitate a constant update of its information. A set of services designed to recommend specific resources in relation to a determined profile of user is provided. Additionally, a discussion about how the ALTER-NATIVA knowledge management approach can be applied to industrial environments is presented.     

Vinyl ester/clay based nanocomposites: a complementary study of vinyl ester polymerization by hyphenated rheo‐Fourier transform infrared and separate rheology/Fourier transform infrared measurements

This work is focused on the complementary information obtained from advanced in situ Fourier transform infrared (FTIR) spectroscopy and rheological measurements to correlate the structural changes with rheological properties upon polymerization of different organically modified montmorillonite clay/vinyl ester composites prepared by in situ polymerization. The microstructure and morphology of the nanocomposites were examined by X‐ray diffraction and transmission electron microscopy. The effect exerted by the presence of organic clay on the polymerization reaction of a vinyl ester based polymer matrix was evaluated. In situ and ex situ rheo‐FTIR measurements were compared to demonstrate the accuracy of this technique. © 2013 Society of Chemical Industry     

Playing on a holo-stage: towards the interaction between real and virtual performers

This paper presents a number of ongoing projects that will be valuable to the development of technologies for digital theatre and performance. This paper describes an EU ACTS project called RESOLV which develops an integrated system to capture real 3D environments using laser and video. Current research on motion tracking, statistical behaviour modelling and simulation is presented, and possible applications, both for visual augmentation and audio generation, are discussed. The projects presented were not originally designed in the domain of theatre and performance, however their results and output seem to be well suited to be applied in this area.