The influence of porcine growth hormone on muscle fibre characteristics, metabolic potential and meat quality

Treatment of lean female pigs with porcine growth hormone (placebo or 80 μg pGH per kg body weight per day) for 6 weeks from 50 kg to 86 kg body weight did not change the frequency and the percentage area of muscle fibre types (ST, FTa and FTb fibres) and muscle capillarity of M. longissimus dorsi, M. gluteus medius and M. psoas major. The mean fibre cross-sectional area increased 5·3%, albeit nonsignificantly, corresponding to a 6·6% increase in carcass meat. The muscles contained slightly less dry matter and protein, and the lipid content of M. longissimus dorsi decreased 19% after pGH treatment. In the backfat the fatty acid composition changed towards a higher ratio of polyunsaturated to saturated fatty acids. The pH_u of M. longissimus dorsi was unaltered, while the pH₄₅ was reduced by pGH. The muscle glycogen level and the activity of lactate dehydrogenase (LDH), citrate synthetase (CS), and 3-OH-acyl-CoA-dehydrogenase (HAD) of M. longissimus dorsi were unchanged by pGH. The haem pigment, shear force, sarcomere length and eating quality of loin chops, were unaffected by pGH treatment. The results show that pGH treatment did not change the muscle characteristics in a way that affects the meat quality of M. longissimus dorsi in lean female pigs.     

tweezercalib 2.0: Faster version of MatLab package for precise calibration of optical tweezers

We present a vectorized version of the MatLab (MathWorks Inc.) package tweezercalib for calibration of optical tweezers with precision. The calibration is based on the power spectrum of the Brownian motion of a dielectric bead trapped in the tweezers. Precision is achieved by accounting for a number of factors that affect this power spectrum, as described in vs. 1 of the package [I.M. Toli?-Nørrelykke, K. Berg-Sørensen, H. Flyvbjerg, Matlab program for precision calibration of optical tweezers, Comput. Phys. Comm. 159 (2004) 225-240]. The graphical user interface allows the user to include or leave out each of these factors. Several “health tests” are applied to the experimental data during calibration, and test results are displayed graphically. Thus, the user can easily see whether the data comply with the theory used for their interpretation. Final calibration results are given with statistical errors and covariance matrix.

New version program summary

Title of program: tweezercalibCatalogue identifier: ADTV_v2_0Program summary URL:http://cpc.cs.qub.ac.uk/summaries/ADTV_v2_0Program obtainable from: CPC Program Library, Queen's University of Belfast, N. IrelandReference in CPC to previous version: I.M. Toli?-Nørrelykke, K. Berg-Sørensen, H. Flyvbjerg, Comput. Phys. Comm. 159 (2004) 225Catalogue identifier of previous version: ADTVDoes the new version supersede the original program: YesComputer for which the program is designed and others on which it has been tested: General computer running MatLab (Mathworks Inc.)Operating systems under with the program has been tested: Windows2000, Windows-XP, LinuxProgramming language used: MatLab (Mathworks Inc.), standard licenseMemory required to execute with typical data: Of order four times the size of the data fileHigh speed storage required: noneNo. of lines in distributed program, including test data, etc.: 135 989No. of bytes in distributed program, including test data, etc.: 1 527 611Distribution format: tar. gzNature of physical problem: Calibrate optical tweezers with precision by fitting theory to experimental power spectrum of position of bead doing Brownian motion in incompressible fluid, possibly near microscope cover slip, while trapped in optical tweezers. Thereby determine spring constant of optical trap and conversion factor for arbitrary-units-to-nanometers for detection system.Method of solution: Elimination of cross-talk between quadrant photo-diode's output channels for positions (optional). Check that distribution of recorded positions agrees with Boltzmann distribution of bead in harmonic trap. Data compression and noise reduction by blocking method applied to power spectrum. Full accounting for hydrodynamic effects: Frequency-dependent drag force and interaction with nearby cover slip (optional). Full accounting for electronic filters (optional), for “virtual filtering” caused by detection system (optional). Full accounting for aliasing caused by finite sampling rate (optional). Standard non-linear least-squares fitting. Statistical support for fit is given, with several plots facilitating inspection of consistency and quality of data and fit.Summary of revisions: A faster fitting routine, adapted from [J. Nocedal, Y.x. Yuan, Combining trust region and line search techniques, Technical Report OTC 98/04, Optimization Technology Center, 1998; W.H. Press, B.P. Flannery, S.A. Teukolsky, W.T. Vetterling, Numerical Recipes. The Art of Scientific Computing, Cambridge University Press, Cambridge, 1986], is applied. It uses fewer function evaluations, and the remaining function evaluations have been vectorized. Calls to routines in Toolboxes not included with a standard MatLab license have been replaced by calls to routines that are included in the present package. Fitting parameters are rescaled to ensure that they are all of roughly the same size (of order 1) while being fitted. Generally, the program package has been updated to comply with MatLab, vs. 7.0, and optimized for speed.Restrictions on the complexity of the problem: Data should be positions of bead doing Brownian motion while held by optical tweezers. For high precision in final results, data should be time series measured over a long time, with sufficiently high experimental sampling rate: The sampling rate should be well above the characteristic frequency of the trap, the so-called corner frequency. Thus, the sampling frequency should typically be larger than 10 kHz. The Fast Fourier Transform used works optimally when the time series contain n² data points, and long measurement time is obtained with n>12-15. Finally, the optics should be set to ensure a harmonic trapping potential in the range of positions visited by the bead. The fitting procedure checks for harmonic potential.Typical running time: SecondsUnusual features of the program: NoneReferences: The theoretical underpinnings for the procedure are found in [K. Berg-Sørensen, H. Flyvbjerg, Power spectrum analysis for optical tweezers, Rev. Sci. Ins. 75 (2004) 594-612].     

合作型的全球城市——德国城市体系研究及其对上海的启示

与美国,英国和日本不同,德国作为世界上最重要的经济体之一,并没有一个顶级的全球城市.对德国城市体系的分析显示,德国拥有一个由多个重要城市构成的城市体系网络,城市间存在良好的劳动分工和专业化协作,且具有高度的交通可达性.这种城市体系结构加强了区域之间的竞争,有利于发现更好的问题解决方法,而且与单一的全球城市相比具有较低的拥堵成本.这种城市体系并没有影响甚至是强化了德国企业在世界高端产品生产上的优势地位,德国企业也并没有因缺少单一的全球城市而丧失了全球经济的控制权.因而,从成效出发,德国城市系统可以被看作是一个合作型的全球城市.这样的经验为上海建设国际经济、金融、贸易和航运中心提供了新思路,未来的国际经济中心城市应该突破上海行政界限,定位为长三角地区合作共建的全球城市.为此,长三角城市间要合理分工和专业化协作,以市场机制为根本实现路径,并进一步提高城市间的交通可达性.     

Effects of Particle Crushing in Stress Drop-Relaxation Experiments on Crushed Coral Sand

Stress relaxation and stress drop-relaxation tests have been performed to complement a test series performed to study strain rate, creep, and stress drop-creep effects on crushed coral sand. Drained experiments with constant effective confining pressure of 200 kPa were performed in which triaxial specimens of crushed coral sand were loaded to initial stress differences of 500, 700, and 900 kPa, followed by stress drops of 0, 100, 200, 300, and 400 kPa at which points the axial strains were kept constant while the axial stress relaxation and the volumetric strains were observed. The stress drops produced delays in initiation of stress relaxation that were proportional with the magnitudes of the stress drops. The experiments show that sands do not exhibit classic viscous effects, and their behavior is indicated as “nonisotach,” while the typical viscous behavior of clay is termed “isotach.” Thus, there are significant differences in the time-dependent behavior patterns of sands and clay. A mechanistic picture of time effects in sands is proposed.     

Aequorin-based functional assays for G-protein-coupled receptors,ion channels,and tyrosine kinase receptors

Aequorin is a photoprotein originating from jellyfish, whose luminescent activity is dependent on the concentration of calcium ions. Due to the high sensitivity and low background linked to luminescent assays, as well as to its absence of toxicity and its large linear dynamic range, aequorin has been used as an intracellular calcium indicator since its discovery in the early 1960s. The first applications of aequorin involved its microinjection in cells. The cloning of its gene in 1985 opened the way to the stable expression of aequorin in cell lines or even entire organisms. Here we present the validation of aequorin as a functional assay for the screening of G-protein-coupled receptors, ion channels, and tyrosine kinase receptors, as well as for their pharmacological characterization in agonist and antagonist detection assays. We optimized our cell suspension-based assay and determined that the most sensitive assay was performed at room temperature, with mitochondrially expressed aequorin and using coelenterazine derivative h for reconstitution of aequorin. The robustness of the assay and the current availability of luminometers with integrated injectors allow aequorin to fit perfectly with high throughput functional assays requirements.     

Configurations of NPD – Production Interfaces and Interface Integration Mechanisms

This paper describes and illustrates different configurations of the interface between new product development and production processes, including both intra–firm and inter–firm interfaces. These configurations are partly based on a process view of product innovation and partly on a structural view of product innovation. In addition to this typology of interfaces some integration mechanisms are described. The typology will serve as a basis for further research aimed at identifying consistent configurations of the different types of integration mechanisms that are available for industry to increase the effectiveness and efficiency of product innovation processes.     

Introduction by the conference editor

This paper deals with the problem of structuring the confusing pattern of problems of computerization. The approach to finding a relevant frame of reference for a discussion of the impact of computerization on qualities of work is to make some clear and basic distinctions by asking some very general questions and the look at the typical answers.The author's main argument is, that this approach leads to the following distinctions: Firstly, the distinction between three dimensions in the concept of work, which leads to the distinction between the structural, the functional, and the ethical aspects of computerization. Secondly, the distinction between three levels of society, which leads to the distinction between the societal, the social, and the psycho-physical aspects of computerization. Thirdly, the distinction between three types of questions regarding work, which leads to the distinction between the quantitative, the qualitative, and the utility aspects of computerization. However, these three times three times three aspects may be regarded by at least five different interest groups each asking at least four different interest groups each asking at least four different questions. No wonder that the problem is complex and the discussion may be confusing.The point is, that to find a reasonable pattern in this mess of problems we have to choose a human point of view and use a general framework of qualities of life for structuring the complex of problems. Essential to the human being are the relations between man and environment, and working environment should be regarded as a subsystem of environment in general. By doing that we find, that the change in the distribution of responsibility of doing the work between man and machine is the most remarkable impact of computerization.     

Isolation,characterization, and quantitative analysis of Microviridin J,a new Microcystis metabolite toxic to Daphnia

This paper describes the purification and characterization of microviridin J, a newly discovered metabolite of Microcystis that causes a lethal molting disruption in Daphnia spp., upon ingestion of living cyanobacterial cells. Microviridin J consists of an acetylated chain of 13 amino acids arranged in three rings and two side chains. Unlike other known isoforms of microviridin, microviridin J contains arginine that imparts a unique solution conformation characterized by proximal hydrophobic interactions between Arg and other regions of the molecule. This eventually results in the formation and stabilization of an additional ring system. Microviridin J potently inhibits porcine trypsin, bovine chymotrypsin, and daphnid trypsin-like proteases. The activity against trypsin is most likely due to Arg and its distinctive conformational interactions. Overall, the data presented for microviridin J emphasize once again the ability of cyanobacteria to produce numerous and potent environmental toxins.