pH, TEMPERATURE AND HYDRATION KINETICS OF FABA BEANS (VICIA FABA L.)

Soaking kinetics of dry faba beans was studied in the temperature range 20–65C, and at pH 3, 6, 9 and 12 by the method of weight gain until equilibrium conditions were attained. Increasing the soaking temperature from 20 to 35C resulted in a small increase in hydration rate constants as compared to that realized with further increase to 50 and 65C in the investigated range of pH. Soaking at 65C decreased the amount of water absorbed by the beans and the time at which equilibrium was attained. Beans soaked at alkaline pH exhibited higher hydration rates especially when hydration was carried out at ≥ 50C     

乳固形物浓度对浓缩型酸乳酪流变性质和感官品质的影响

研究了乳固形物浓度对浓缩型酸乳酪(Labneh)品质的影响，对采用传统工艺制备的新鲜Labneh样品分别进行流变分析和感官评定。流变分析表明，浓缩型酸乳酪具有较强的弹性结构；随着乳固形物浓度的增加，浓缩型酸乳酪的硬度和粘性都会明显下降；扫描电镜观察微观结构表明，其结构致密而均匀，而且酪蛋白颗粒的尺寸比对照组小；感官评定表明，当乳固形物浓度提高到接近质量分数15．50％时浓缩型酸乳酪细腻光滑、可涂抹性较理想。     

Evaluation of the hepatoprotective and antioxidant effects of <i>Tegillarca granosa</i> flesh body extract against potassium bromide toxicity via targeting the histomorphometry,chromosomal and expressions of TGF-β1, VEGF and COX-2 genes in rats

The hepatotoxic effect of potassium bromide (KBr) on rat liver tissues were determined, as well as the potential protective effect of Tegillaraca granosa (T. granosa) flesh body extract. Twenty adult male albino rats were equally distributed into four groups; Group (I) treated with physiological saline (control group), Group (II) was orally gavaged by 200 mg/kg of T. granosa body extract day after day, Group (III) was intoxicated by KBr (150 mg/kg bwt day after day orally) and finally, Group (IV) was given a combination of T. granosa flesh body extract plus KBr with similar doses in the second and third groups. At the end of one month, blood, liver tissue and bone marrow samples were collected to be used for the required laboratory examinations. In response to KBr toxicity, there was a significant increase in serum antioxidant biomarkers, which was accompanied by a significant change in hepatocyte ultrastructure and a significant change in carbohydrate and protein levels within the liver organ. In addition, KBr intoxication resulted in a substantial increase in the incidence of chromosomal aberrations such as holes, splits, deletions, fragments, ploidy, and ring chromosomes, as well as significant upregulation of TGF-1, VEGF, and COX-2 gene expression. The hepatotoxic effect of KBr was counteracted by treatment with T. granosa flesh body extract. T. granosa flesh body extract has a curative antioxidant and numerous protective effects against KBr hepatotoxicity.     

Ameliorative effects of melatonin and zinc oxide nanoparticles treatment against adverse effects of busulfan induced infertility in male albino mice

Testicular damage is one of the most hazardous effects as it’s associated with azoospermia. Busulfan (Bu) is a highly toxic chemotherapeutic drug that affects testis. Thirty male Swiss albino mice divided into six groups of 5 animals each. Control (oral 0.9% saline daily for 75 days); Mel (20 mg/kg/day orally for 30 days); ZnO NPs (5 mg/kg/day i.p. for 30 days); BU (single i.p. injection of 40 mg/kg and then left for 45 days); BU + Mel (single 40 mg/kg dose of BU and left for 45 days followed by 20 mg/kg/day Mel for 30 days); BU + ZnO NPs (single dose of 40 mg/kg of BU and left for 45 days, then 5 mg/kg/day ZnO NPs for 30 days). Preparation and Characterization of ZnO NPs. Specimens from testis prepared for ultrastructural investigations using TEM after Masson’s trichrome and toluidine blue staining. BU induced histological and ultrastructural damage of the testis. Moreover, the present results could be concluded that Mel or ZnO NPs can protect the testicular tissue against ultrastructural alterations induced by BU by its antioxidant and anti-apoptotic effects.     

Manufacturing, properties and shelf life of labneh: a review

Labneh is a concentrated yogurt widely produced and consumed in the Middle Eastern countries and Balkans.
This review focuses attention on the different manufacturing methods of labneh and its quality in terms of microbiology, rheology, sensory and nutrition. The shelf life of this healthy milk-derived product is also discussed in this review.     

Anti-inflammatory and antioxidant potential capacities of AD-MSCs and BM-MSCs in suppressing pancreatic β-cells auto-immunity and apoptosis in rats with T1DM induced model

Since Type 1 diabetes (T1DM) occurs when β-cells mass is reduced to less than 20% of the normal level due to autoimmune destruction of cells resulting in the inability to secrete insulin, preservation or replenishment of the functional β-cells mass has become a major therapeutic focus for this diabetic type treatment. Thus, this 4-week work plan was designed to determine which mesenchymal stem cells (MSCs) type is more appropriate to alleviate pancreatic hazards resulting from diabetes induction; via tracking a comparative study between MSCs derived from adipose tissue (AD-MSCs) and from bone marrow (BM-MSCs) in management of T1DM considering their immunomodulatory, anti-apoptotic and antioxidative roles. Rats were divided randomly into 4 groups; control, STZ-diabetic (D), D+AD-MSCs, and D+BM-MSCs groups. Both stem cells types in this study were allogenic. Herein, both oxidative stress and antioxidant markers were evaluated using colorimetric analysis, while inflammatory, immune and apoptotic markers were assessed through flow cytometric analysis. Results showed that diabetic rats treated with either AD-MSCs or BM-MSCs exhibited marked pancreatic antioxidant and anti-inflammatory activities that were able to initiate pancreatic immunomodulation and reducing β-cells apoptotic death, thus, help to restore their normal insulin secretion and hypoglycemic abilities. However, AD-MSCs injection was shown to be superior as a pancreatic regenerative tool in overcoming diabetes; owing to their marked antioxidant, anti-inflammatory, immunomodulatory, and anti-apoptotic characteristics over BM-MSCs treatment.     

Investigation of the antioxidant defensive role of both AD-MSCs and BM-MSCs in modulating the alteration in the oxidative stress status in various STZ-diabetic rats’ tissues

Diabetes mellitus (DM) could negatively affect patients’ health via inducing a lot of serious functional hazards in many tissues’ cells at molecular levels. Recently, many scientists had proposed stem cell therapy being an appropriate alternative treatment protocol for numerous health threatening issues including diabetes. Therefore, the current study was designed to investigate the antioxidant potentiality of two MSCs types in alleviating tissues’ oxidative stress dramatic elevation resulting as a consequence of Type 1 DM induction. In our 4 weeks study, animals were divided into four groups: control group, STZ-diabetic group (D), D+AD-MSCs group and D+BM-MSCs group. Data reported that diabetic rats treated with either AD-MSCs or BM-MSCs exhibited a marvelous body tissues (Pancreas, Liver and Kidney) enhancing capabilities in attenuating the oxidative stress status; as evidenced by XO, ROS, and MDA levels down-regulation; with a general concomitant elevation in the antioxidants’ content; evidenced by many enzymatic and non-enzymatic antioxidants up-regulation; relative to the diabetic untreated group. Interestingly, comparing both treatments with each other and to control group, most of the measured parameters were reverted back to near normal levels after AD-MSCs injection; which clearly point out their stunning health benefits and superiority as anti-diabetic agent in overcoming different tissues’ complications; owing to their marked cytoprotective and regenerative potentialities.