Locus heterogeneity in autosomal dominant congenital external ophthalmoplegia (CFEOM)

An autopsy case of retroperitoneal leiomyosarcoma of a centenarial male is described. He complained of abdominal pain in his left side 1 month before his death. Ultrasonography and computed tomography revealed a large mass in the left abdominal cavity. At autopsy, a circumscribed mass, 15 cm in diameter, was found in the left-upper abdominal cavity, and seemed to be derived from the retroperitoneum in an exophytic manner. Histologically, spindle cells with slight eosinophilic cytoplasm and blunt-ended nuclei showed interwoven fascicular growth, and mitotic figures were easily encountered. Immunohistochemically, the tumor cells were labeled by alpha-smooth muscle actin and vimentin. To our knowledge, this case of leiomyosarcoma has presented in the oldest patient ever reported. The rarity of soft tissue sarcomas in the extremely elderly is also discussed.     

Multiscale Effects on Spatial Variability Metrics in Global Water Resources Data

Spatial scales and methods for dealing with scale have been widely discussed in the water resources literature. Different spatial processes operate at different scales so interpretations based on data from one scale may not apply to another. Understanding the behavior of phenomena at multiple-scales of data aggregation is thus imperative to accurate integrations of data and models at different geographic resolutions. This study tests theoretical concepts of scale by presenting empirical results of multiscale GIS and statistical analyses on gridded water-availability, water use and population data for the Danube Basin in Europe, with results corroborated by similar tests in the Ganges (South Asia) and Missouri (North America) Basins. Fine-resolution datasets were aggregated to coarser grid sizes and standard statistical measures of spatial variability were computed. Statistical analysis of spatial variability demonstrated two distinctly different cases for unscaled and scaled variables. Results show that variance (and standard deviation) in unscaled variables like freshwater supply, use and population increases at coarser scales—contrary to the common assumption of decreasing variability as grid-cell size increases. On the other hand, a decreasing trend in variability with scale is noted for variables scaled to area or population (like population density, water availability per capita etc.). Moreover, relationships between variability and scale show strong non-linear trends. No mention of these relationships has been found in the water resources or socio-economic literature for scale and variability. Regression analyses suggest that power functions are the most appropriate model to fit trends in increasing variability at multiple scales. These results can be applied to interpretations of water-stress and water scarcity data and their locations relative to water sources or topographic barriers.     

Purification and characterization of a novel glucoamylase from Fusarium solani

Thermostable enzymes are currently being investigated to improve industrial processes of starch saccharification. A novel glucoamylase was purified to electrophoretic homogeneity from the culture supernatant of Fusarium solani on a fast protein liquid chromatographic system (FPLC). The recovery of glucoamylase after gel filtration on FPLC was 31.8% with 26.2-fold increase in specific activity. The enzyme had a molecular mass of 40 kDa by SDS-PAGE and 41 kDa by gel filtration. The glucoamylase exhibited optimum activity at pH 4.5. The K_cat and K_m were 441/min and 1.9 mg/ml, respectively, for soluble starch, specificity constant (K_cat/K_m) was 232. The enzyme was thermally stable at 50 °C and retained 79% activity after 60 min at this temperature. The half-life of the enzyme was 26 min at 60°C. The enzyme was slightly stimulated by Cu²⁺ and Mg²⁺ and strongly inhibited by Hg²⁺, Pb²⁺, Zn²⁺, Ni²⁺ and Fe³⁺.     

Volatile flavour components of garden cress

Using previously reported methods of sample preparation an essence of garden cress was obtained which proved to contain only 11 components. Eight have been identified, all glucosinolate degradation products (isothiocyanates and nitriles), and provide over 95% of the sample. Benzyl derivatives, from glucotropaeolin, are the most abundant. No thiocyanates were obtained.     

Volatile flavour components of coriander leaf

Using previously reported methods of sample preparation an essence of coriander leaf was obtained which proved to contain over 25 components. Most of these have been identified, with aliphatic aldehydes providing nearly 90% of the sample. C₁₂ aldehydes are the most abundant.     

Physical and chemical modification of distillery sludge for Pb(II) biosorption

The waste distillery sludge from sugar-cane industry was pretreated physically (boiled, heated and autoclaved) as well as chemically (HCl, H(2)SO(4), H(3)PO(4), NaOH, Ca(OH)(2), Al(OH)(3), C(6)H(6), HCHO, CH(3)OH and C1(2)H(25)OSO(3)Na (sodium dodecyl sulphate (SDS)) for assessing the comparative sorption capacity of untreated and modified distillery sludge for Pb(II) biosorption from aqueous solutions. Experiments were conducted in shake flasks on a batch basis to access the effect of different experimental parameters such as pH, biosorbent dosage, biosorbent size, initial Pb(II) concentration and contact time. The uptake capacity 'q' (mg/g) of untreated and pretreated distillery sludge was in following order: NaOH (51.29+/-1.21)>HCl (49.82+/-1.22)>HCHO (49.56+/-1.14)>H(2)SO(4) (47.71+/-1.20)>HgCl(2) (45.32+/-1.06)>Ca(OH)(2) (44.01+/-1.18)>MeOH (43.73+/-1.23)>C(6)H(6) (42.72+/-1.19)>H(3)PO(4) (42.01+/-1.17)>SDS (40.87+/-1.27)>autoclaved (40.23+/-1.24)>Boiled (39.95+/-1.19)>heated (38.87+/-1.32)>Al (OH)(3) (38.30+/-1.14)>untreated (37.76+/-1.21). In further parameter studies, the optimized biosorbent size was 0.250 mm at pH 5 and best dose was 0.05 g of biosorbent. The applicability of the Langmuir and Freundlich models for sorption process was tested and best fitted model was Langmuir with the coefficient of determination (R(2)) value, 0.97, the process followed second order kinetic mechanism.     

Fabrication of different geometry cutting tools and their effect on the vertical micro-grinding of BK7 glass

With the demand for microstructures of not only with diversified shape but also of reduced dimension on glass, fabrication of polycrystalline diamond (PCD) tool/microelectrodes with different shape has become important. However, to date, fabrication of different shapes in single setup is not possible and also needs special indexing attachment. To solve this problem, in this study, a specially designed block containing three v-slots of 60°, 90°, and 120° has been designed and fabricated using wire cut. Thereafter with the help of block electro-discharge machining method and using this specially designed block, different shapes of microelectrodes with symmetrical and non-symmetrical section has been fabricated. This study also investigates the feasibility of using these different geometry PCD tool for micro-grinding of BK7 glass. In this context, a relative study on the micro-grinding performance of four different geometry tools (circular, D-shaped, triangular, and square) has been carried out. It has been observed that among the different shaped tools, D-shaped tool experienced lowest cutting force along x- and y-axes where as triangular tool faced lowest force along z-axis, and highest cutting forces were found to be experienced by square tool. Average and maximum roughness of machined surface was found to be improved from circular to others tool except triangular one. But, it was also observed that side surface started to deteriorate from circular to other tool due to edge wear. In case of tool wear, square and triangular tool experienced more wear than circular and D-shaped tool due to their frequent edge blunting or rounding effect. Finally, among four different geometry tools, D-shaped tool was considered to provide better performance in terms of the achieved surface finish, tool wear, and cutting force analysis.     

Effect of heat treatments on the phytic acid content of maize products

Processing of maize (Zea mays L fresh and dry) for the production of various traditional products results in the loss of phytic acid. Fresh mature corn contains less phytic acid (1·71 g kg^?1) than dry corn (7·15–7.60 g kg^?1). The loss of phytic acid varies from 18·1 to 46·7% for fresh maize and from 11·5 to 52·6% for dry maize respectively among the heat treatments given. From a nutritional viewpoint, consumption of maize as chapati and after roasting in a sand bath or on charcoal will improve the availability of minerals.     

Ameliorating Effects of Exogenously Applied Proline on Seed Composition,Seed Oil Quality and Oil Antioxidant Activity of Maize (Zea mays L.) under Drought Stress

This study was carried out to appraise whether or not the exogenous application of a potential osmoprotectant, proline, could ameliorate the adverse effects of drought stress on maize seed and seed oil composition, as well as oil antioxidant activity. Water stress reduced the kernel sugar, oil, protein and moisture contents and most of the seed macro- and micro-elements analyzed in both maize cultivars but it increased the contents of seed fiber and ash. Water stress increased the oil oleic acid content with a subsequent decrease in the amount of linoleic acid, resulting in an increased oil oleic/linoleic ratio for both maize cultivars. However, no variation was observed in oil stearic and palmitic acids content due to water stress. A considerable drought induced an increase in seed oil α-, γ-, δ- and total tocopherols and flavonoids were observed in both maize cultivars. However, oil phenolic and carotenoid content as well as 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging activity decreased. Foliar-applied proline significantly increased the content of seed sugar, oil, protein, moisture, fiber and ash in both maize cultivars under well irrigated and water deficit conditions. Furthermore, exogenous application of proline increased the oil oleic and linoleic acid contents. The concentrations of antioxidant compounds namely phenolics, carotenoids, flavonoids and tocopherols estimated in the seed oil increased due to foliar-applied proline under water deficit conditions that was positively correlated with the enhanced oil DPPH free radical scavenging activity. Moreover, the increase in the contents of these antioxidant compounds and oil antioxidant activity due to the foliar application of proline was noted to be more pronounced under water deficit conditions.