In vivo Kinetic Biodistribution of Nano‐Sized Outer Membrane Vesicles Derived from Bacteria

Evaluation of kinetic distribution and behaviors of nanoparticles in vivo provides crucial clues into their roles in living organisms. Extracellular vesicles are evolutionary conserved nanoparticles, known to play important biological functions in intercellular, inter‐species, and inter‐kingdom communication. In this study, the first kinetic analysis of the biodistribution of outer membrane vesicles (OMVs)—bacterial extracellular vesicles—with immune‐modulatory functions is performed. OMVs, injected intraperitoneally, spread to the whole mouse body and accumulate in the liver, lung, spleen, and kidney within 3 h of administration. As an early systemic inflammation response, increased levels of TNF‐α and IL‐6 are observed in serum and bronchoalveolar lavage fluid. In addition, the number of leukocytes and platelets in the blood is decreased. OMVs and cytokine concentrations, as well as body temperature are gradually decreased 6 h after OMV injection, in concomitance with the formation of eye exudates, and of an increase in ICAM‐1 levels in the lung. Following OMV elimination, most of the inflammatory signs are reverted, 12 h post‐injection. However, leukocytes in bronchoalveolar lavage fluid are increased as a late reaction. Taken together, these results suggest that OMVs are effective mediators of long distance communication in vivo.     

裂缝性孔隙介质频变AVAZ反演方法研究进展

由于尺度的限制,利用地震方法进行裂缝参数定量表征一直是地球科学的难题。基于裂缝性孔隙介质动态等效理论的频变AVAZ反演方法给出了研究思路。首先介绍了裂缝性孔隙介质的等效介质理论研究进展,分析比较了各种裂缝性储层岩石静态等效介质模型和动态等效介质模型;然后给出了基于Chapman模型的裂缝参数和渗透率定量表征的正问题数学模型以及反演问题求解的方法和策略,以及基于模型数据的数值计算结果;最后讨论了频变AVAZ反演方法目前存在的问题和进一步研究方向。     

Properties and applications of β‐glycosidase from Bacteroides thetaiotaomicron that specifically hydrolyses isoflavone glycosides

To modify the glycan part of glycosides, the gene encoding β‐glycosidase was cloned from Bacteroides thetaiotaomicron VPI‐5482. The cloned gene, bt_1780, was expressed in Escherichia coli MC1061 and the expressed enzyme was purified using Ni‐NTA affinity chromatography. The purified enzyme, BTBG, showed optimal activity at 50 °C and pH 5.5. Interestingly, this enzyme did not have any hydrolysing activity on ordinary β‐linkage–containing substrates such as xylobiose, lactose and cello‐oligosaccharide, but specifically hydrolysed isoflavone glycosides such as daidzin, genistin and glycitin. Compared to a commercial beta glucosidase, BTBG selectively hydrolysed isoflavone glycosides in soybean extract mixture solution. These results suggest that BTBG may be a specialized enzyme for the hydrolysis of glycosides and that the substrate specificity of BTBG is applicable for the bioconversion of isoflavone glycosides in the food industry.     

简易棋盘格图像标定系统应用

棋盘格图像在摄像机标定中有非常广泛的应用,对于复杂环境且测量精度要求非常高的场合,会需要特定的且有一定复杂度的算法,但通常情况下,使用这样的标定系统是没有必要的,且会让初次应用的人觉得困难。这里提供一套应用简单,快速,对棋盘格图像具有旋转不变性,良好的鲁棒性,且对非复杂环境具有高精度的标定系统。所有算法均是在前辈们的算法上稍作改变而来,应用效果非常好。