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LA Knapp E Lehmann L Hennes ME Eberle DI Watkins 《Canadian Metallurgical Quarterly》1997,50(2):170-177
Skeletal development of transgenic mice with a type II collagen mutation was analyzed and compared with wild-type littermates. The single base substitution in Col2a1 resulted in a glycine to serine mutation within the helical domain and corresponded to one previously identified in a patient with the lethal human chondrodysplasia, hypochondrogenesis (Horton et al. [1992] Proc. Natl. Acad. Sci. U.S.A. 89:4583-4587). Skeletal staining of embryos from 14.5 through 18.5 days of gestation demonstrated a dwarf phenotype in the transgenic embryos, most notably short limb bones and vertebral column that was first detected at 15.5 days post-coitus. In addition to the reduced length, the extent of ossification was less in the transgenic mice. The architecture of the long bone growth plate was abnormal in the transgenic tissue, in particular there was no discernible proliferative zone. There were few stacks of characteristically flattened cells and the overall length of the growth plate in the mutant embryos was reduced. At the ultrastructural level, there were fewer collagen fibrils present in the transgenic mouse cartilage compared to that of wild-type littermates. Ultrastructural localization of collagen types II, IX and XI revealed a similar pattern between the transgenic and wild-type pups, suggesting that the collagen fibrils present in the matrix of littermates with both phenotypes had a similar composition. Skeletal analysis and cartilage histochemistry indicated that effect of the type II collagen mutation was to reduce the density of the collagen fibrils within the cartilage matrix which was associated with delayed bone formation and resulted in a short-limbed phenotype. 相似文献
3.
There are three selenium-containing proteins in human plasma: glutathione peroxidase (GSH-Px-P), albumin and selenoprotein Ph, the human analogue to selenoprotein P from rat plasma. Selenoprotein Ph was separated from the two other selenium-containing proteins by Heparin Sepharose chromatography and was shown to have about 60-70% of the total plasma selenium, while both GSH-Px-P and albumin contain about 15%. A 2588-fold purification from human plasma was achieved by using a four-step procedure. SDS-PAGE of the purified selenoprotein revealed, besides one contaminant selenium-free protein band at about 70 kDa, one selenium-containing band ranging from 54 to 67 kDa with a maximum at 63 kDa. This microheterogeneity, also recognized by IEF, may be due to the glycprotein nature of the selenoprotein Ph. The determination of the molecular mass of the native protein varied from 65 kDa using gel filtration on Fraktogel HW 55 to 89 kDa on Sephacryl S-200 HR, suggesting an interaction between the gel-matrices and selenoprotein Ph. 相似文献
4.
Schubert U.-A. Anderle F. Spengler J. Zühlke J. Eberle H.-J. Grasselli R.K. Knözinger H. 《Topics in Catalysis》2001,15(2-4):195-200
Titania-supported vanadia catalysts were modified by addition of antimony oxide for application in o-xylene selective oxidation to phthalic anhydride. It was shown that active and selective catalysts can be prepared by ball-milling mixtures of powders of TiO2, V2O5and Sb2O3followed by calcination. X-ray photoelectron spectroscopy proves the formation of highly dispersed overlayers of vanadium oxide and antimony oxide, in which V5+is partially reduced to lower oxidation states and Sb3+is partially oxidized to Sb5+. Antimony oxide segregated into the outermost surface layers. It is therefore inferred that the presence of the antimony oxide modifier spatially separates V–O species and leads to site isolation which may be responsible for the positive effect of the modifier for the catalyst's selectivity. 相似文献
5.
André M. Eberle 《International Journal of Parallel, Emergent and Distributed Systems》2016,31(5):405-419
Traditionally, computer programs have been developed using the sequential programming paradigm. With the advent of parallel computing systems, such as multicore processors and distributed environments, the sequential paradigm became a barrier to the utilisation of the available resources, since the program is restricted to a single processing unit. To address this issue, we propose a transparent automatic parallelisation tool with a binary rewriter. The steps of our approach are: the disassembly of the Intel x86 application, its transformation into an intermediary language; the analysis of this intermediary representation to obtain the flow and dependency graphs; the partitioning of the application into parallel units, using the obtained graphs; and, finally, the reassembly of the application back into the original Intel x86 architecture. By transforming the compiled application software, we aim at obtaining a program which can better explore the parallel resources, with no extra effort required from users or developers. 相似文献
6.
Juha K. Laurila Daniel Gatica-Perez Imad Aad Jan Blom Olivier Bornet Trinh Minh Tri Do Olivier Dousse Julien Eberle Markus Miettinen 《Pervasive and Mobile Computing》2013,9(6):752-771
This paper presents an overview of the Mobile Data Challenge (MDC), a large-scale research initiative aimed at generating innovations around smartphone-based research, as well as community-based evaluation of mobile data analysis methodologies. First, we review the Lausanne Data Collection Campaign (LDCC), an initiative to collect unique longitudinal smartphone dataset for the MDC. Then, we introduce the Open and Dedicated Tracks of the MDC, describe the specific datasets used in each of them, discuss the key design and implementation aspects introduced in order to generate privacy-preserving and scientifically relevant mobile data resources for wider use by the research community, and summarize the main research trends found among the 100+ challenge submissions. We finalize by discussing the main lessons learned from the participation of several hundred researchers worldwide in the MDC Tracks. 相似文献
7.
Rajiv K. Bedi Dr. Danzhi Huang Stefanie A. Eberle Dr. Lars Wiedmer Dr. Pawel Śledź Prof. Amedeo Caflisch 《ChemMedChem》2020,15(9):744-748
The RNA methylase METTL3 catalyzes the transfer of a methyl group from the cofactor S-adenosyl-L-methionine (SAM) to the N6 atom of adenine. We have screened a library of 4000 analogues and derivatives of the adenosine moiety of SAM by high-throughput docking into METTL3. Two series of adenine derivatives were identified in silico, and the binding mode of six of the predicted inhibitors was validated by protein crystallography. Two compounds, one for each series, show good ligand efficiency. We propose a route for their further development into potent and selective inhibitors of METTL3. 相似文献
8.
This paper investigates the mutual impact channels of Germany's major regional policy instrument (GRW) on regional economic development. Different from earlier studies which have predominately focused on a partial assessment of output effects, we explicitly endogenize the factor inputs of the underlying production function. This allows us to comprehensively assess the role of the GRW in driving per capita output, employment, human and physical capital intensities as well as the region's technological growth. The results from a spatial panel vector autoregressive model show that GRW funding has significant positive effects on regional output, the employment rate and human capital intensity. 相似文献
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10.
Crowe JR Shapo BM Stephens DN Bleam D Eberle MJ Ignacio Cespedes E Wu CC Muller DM Kovach JA Lederman RJ O'Donnell M 《IEEE transactions on ultrasonics, ferroelectrics, and frequency control》2000,47(3):672-681
In applications in which Doppler processing is not possible, such as side-looking intravascular imaging systems, decorrelation methods can be used to estimate blood speed. Here, a method is presented measuring relative blood speed using an FIR filter bank to estimate temporal decorrelation rates. It can be implemented in a modern commercially available ultrasound imaging system with no additional hardware. Both simulations and experiments using an intraluminal scanner appropriate for coronary artery applications have tested the system. In this study, the FIR filter bank is contrasted with previous methods, and its utility is further demonstrated with real-time color flow images from a pig model. 相似文献