Two structural adaptations for regulating transmitter release at lobster neuromuscular synapses

The distal accessory flexor muscle (DAFM) in the lobster (Homarus americanus) walking leg consists of 5 muscle fiber bundles. All five bundles, one proximal, one distal, and 3 medial, are innervated by one excitatory and one inhibitory motor neuron. Both neurons release more transmitter on the distal bundle than on the proximal bundle. The aim of our studies was to investigate the structural basis of this differentiation. Thin sections cut at 50 microns intervals showed a similar number of excitatory synapses on the two bundles. Freeze-fracture views of excitatory synapses showed that synapse size, active zone number per synapse, and intramembrane particle density in the postsynaptic membrane are similar proximally and distally. Active zones at synapses on the distal bundle are larger and contain about 50% more large intramembrane particles, which are thought to include the voltage-gated Ca2+ channels that couple the action potential to transmitter release, than their counterparts on the most proximal bundle. This difference in channel number appears to produce a disproportionate increase in the probability of transmitter release sufficient to account for most of the proximal-distal disparity in the amplitude of the excitatory postsynaptic potential. In contrast, staining the inhibitor for antibodies to the inhibitory neurotransmitter, GABA, showed that it forms more varicosities on the distal bundle than on the proximal bundle. Because most of the synapses are located in the varicosities, differences in synapse number likely regulate the proximal-distal disparity in the amount of inhibitory transmitter released. Therefore, the regional differentiation in the amount of transmitter released in the DAFM appears to be based on two distinct mechanisms. In the inhibitor, transmitter release appears to be regulated differentially by differences in synapse number. In the excitor, transmitter release appears to be regulated differentially from a similar number of synapses by differences in active zone structure.     

Catalytic Materials for High-Temperature Combustion

Catalytic combustion, as an alternative to conventional thermal combustion, has received considerable attention during the past decade. Research efforts have been promoted by the need to meet governmental demands concerning pollution and the wish to use energy sources more efficiently. The two main advantages offered by catalytic combustors over flame combustors apply to these goals:

1. Catalytic combustion can be carried out over a wide range of fuel concentrations in air and at low temperatures.
2. These low temperatures result in attaining NO, emission levels substantially lower than possible with conventional combustors.

     

Yield optimization for complex reactor systems

For complex reactions, the choice of reactor type is important for attaining optimum product yields. Strategies for specifying reactor types have beenIn this paper, product yield in a Van de Vusse reaction scheme has been studied for a wide range of the rate constants, in a reactor system consisting     

Comparative Study of the Different Variants of the DV-Hop Based Node Localization Algorithms for Wireless Sensor Networks

Wireless Personal Communications - Node localization is one of the essential services where sensor nodes in the wireless sensor network collaborate to provide location information of sensor nodes...     

Preparation of anchored ceramic coatings on metal substrates: a modified sol-gel technique using colloidal silica sol

A single-step dip-coating technique was developed to produce stable films or washcoats of controlled thickness, surface area and pore-size distribution on alumina whisker-covered metal substrates (both flat plates and monoliths). Dip-coating slurries were prepared by dispersing fine porous powder in a colloidal silica sol. The method provided control over coating thickness and coating properties, such as pore-size distribution and surface area. The coating thickness could be varied between approximately 2 and 40 m by selecting slurry composition and withdrawal speed in the dipping procedure. The pore-size distribution and surface area could be varied by changing type and amount of porous filler material in the dipping slurry. Uniform and bimodal pore-size distributions were obtained using silica and ZSM-5 molecular sieves, respectively, yielding coatings with surface areas between 60 and 400 m²g^–1.     

Virtual modeling of an ankle foot orthosis for correction of foot abnormality

Clubfoot is a challenging structural foot abnormality in babies and generally occurs at birth or during childhood. Due to this the abnormal posturing of the foot causes the babies to turn the ankle inward and walk along the outside edges of the foot. In medical science it is known as “congenital talipes equino varus” (CTEV). Computer modeling of prosthesis and orthosis is a perspective method for optimal design and gait is a terminology related to walking patterns of human subject.     

Development of simulated emotion speech database for excitation source analysis

The work presented in this paper is focused on the development of a simulated emotion database particularly for the excitation source analysis. The presence of simultaneous electroglottogram (EGG) recordings for each emotion utterance helps to accurately analyze the variations in the source parameters according to different emotions. The work presented in this paper describes the development of comparatively large simulated emotion database for three emotions (Anger, Happy and Sad) along with neutrally spoken utterances in three languages (Tamil, Malayalam and Indian English). Emotion utterances in each language are recorded from 10 speakers in multiple sessions (Tamil and Malayalam). Unlike the existing simulated emotion databases, instead of emotionally neutral utterances, emotionally biased utterances are used for recording. Based on the emotion recognition experiments, the emotions elicited from emotionally biased utterances are found to show more emotion discrimination as compared to emotionally neutral utterances. Also, based on the comparative experimental analysis, the speech and EGG utterances of the proposed simulated emotion database are found to preserve the general trend in the excitation source characteristics (instantaneous F0 and strength of excitation parameters) for different emotions as that of the classical German emotion speech-EGG database (EmoDb). Finally, the emotion recognition rates obtained for the proposed speech-EGG emotion database using the conventional mel frequency cepstral coefficients and Gaussian mixture model based emotion recognition system, are found to be comparable with that of the existing German (EmoDb) and IITKGP-SESC Telugu speech emotion databases.