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排序方式: 共有309条查询结果,搜索用时 30 毫秒
1.
DL Lacey JM Erdmann SL Teitelbaum HL Tan J Ohara A Shioi 《Canadian Metallurgical Quarterly》1995,136(6):2367-2376
Interleukin 4 (IL-4) is an immune cytokine that inhibits bone resorption in mice and suppresses osteoclastic cell formation in vitro through an undefined mechanism. In this report, we have established the cellular identity of the IL-4 target cell using a variety of bone marrow/stromal cell coculture methods. Initially, we found that the majority of IL-4's inhibition of osteoclastic cell formation was due to its effect on bone marrow cells, not stromal cells. Consequently, bone marrow macrophages were used as osteoclastic cell progenitors after they had been transiently exposed to IL-4 (48 h), before the addition of stromal cells, 1,25-dihydroxyvitamin D3, and dexamethasone. In this circumstance, IL-4 impaired subsequent osteoclastic cell formation, suggesting that the macrophage may be potentially targeted by many factors known to influence osteoclast formation. Consequently, we discovered that interferon-gamma (IFN gamma), prostaglandin E (PGE), and cell-permeant cAMP analogs also impacted osteoclastic cell formation when used to selectively treat bone marrow macrophages. IFN gamma suppressed osteoclastic cell formation, whereas PGE and cAMP analog treatment led to the formation of significantly enlarged osteoclastic cells. Importantly, PGE antagonized the inhibitory effects of both IL-4 and IFN gamma on the osteoclastic cell-forming potential of bone marrow macrophages. Collectively, these findings establish bone marrow macrophages as osteoclastic cell precursors with the degree of their commitment to the osteoclast pathway sensitive to the effects of soluble mediators, including IL-4, IFN gamma, and PGE. 相似文献
2.
Jaakkola Kelly; Fellner Wendi; Erb Linda; Rodriguez Mandy; Guarino Emily 《Canadian Metallurgical Quarterly》2005,119(3):296
In 2 experiments, bottlenose dolphins (Tursiops truncatus) judged the ordinal relationship between novel numerosities. The dolphins were first trained to choose the exemplar with the fewer number of items when presented with just a few specific comparisons (e.g., 2 vs. 6, 1 vs. 3, and 3 vs. 7). Generalization of this rule was then tested by presenting the dolphins with all possible pairwise comparisons between 1 and 8. The dolphins chose the exemplar with the fewer number of items at levels far above chance, showing that they could recognize and represent numerosities on an ordinal scale. Their pattern of errors was consistent with the idea of an underlying analog magnitude representation. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
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我非常能理解每当发烧友拥有新器材的那种冲动和兴奋的心情,特别是当您面对这样一部技术先进、功能齐全,Dolby Digital、Dolby Digital(AC-3)-ES、dts、 相似文献
5.
Proteins are targeted to the membrane and matrix of peroxisomes by distinct pathways. Recent observations suggest a further route: a subset of peroxisomal membrane proteins might be targeted first to the endoplasmic reticulum, and from there to peroxisomes by vesicle-mediated transport. 相似文献
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Gil Francopoulo Nuria Bel Monte George Nicoletta Calzolari Monica Monachini Mandy Pet Claudia Soria 《Language Resources and Evaluation》2009,43(1):57-70
Optimizing the production, maintenance and extension of lexical resources is one the crucial aspects impacting natural language
processing (NLP). A second aspect involves optimizing the process leading to their integration in applications. With this
respect, we believe that a consensual specification on monolingual, bilingual and multilingual lexicons can be a useful aid
for the various NLP actors. Within ISO, one purpose of Lexical Markup Framework (LMF, ISO-24613) is to define a standard for
lexicons that covers multilingual lexical data.
相似文献
Claudia SoriaEmail: |
8.
High Conductivity in Molecularly p‐Doped Diketopyrrolopyrrole‐Based Polymer: The Impact of a High Dopant Strength and Good Structural Order 下载免费PDF全文
Yevhen Karpov Tim Erdmann Ivan Raguzin Mahmoud Al‐Hussein Marcus Binner Uwe Lappan Manfred Stamm Kirill L. Gerasimov Tetyana Beryozkina Vasiliy Bakulev Denis V. Anokhin Dimitri A. Ivanov Florian Günther Sibylle Gemming Gotthard Seifert Brigitte Voit Riccardo Di Pietro Anton Kiriy 《Advanced materials (Deerfield Beach, Fla.)》2016,28(28):6003-6010
9.
JM Warnecke JP Fürste WD Hardt VA Erdmann RK Hartmann 《Canadian Metallurgical Quarterly》1996,93(17):8924-8928
To study the cleavage mechanism of bacterial Nase P RNA, we have synthesized precursor tRNA substrates carrying a single Rp- or Sp-phosphorothioate modification at the RNase P cleavage site. Both the Sp- and the Rp-diastereomer reduced the rate of processing by Escherichia coli RNase P RNA at least 1000-fold under conditions where the chemical step is rate-limiting. The Rp-modification had no effect and the Sp-modification had a moderate effect on precursor tRNA ground state binding to RNase P RNA. Processing of the Rp-diastereomeric substrate was largely restored in the presence of the "thiophilic" Cd2+ as the only divalent metal ion, demonstrating direct metal ion coordination to the (pro)-Rp substituent at the cleavage site and arguing against a specific role for Mg(2+)-ions at the pro-Sp oxygen. For the Rp-diastereomeric substrate, Hill plot analysis revealed a cooperative dependence upon [Cd2+] of nH = 1.8, consistent with a two-metal ion mechanism. In the presence of the Sp-modification, neither Mn2+ nor Cd2+ was able to restore detectable cleavage at the canonical site. Instead, the ribozyme promotes cleavage at the neighboring unmodified phosphodiester with low efficiency. Dramatic inhibition of the chemical step by both the Rp- and Sp-phosphorothioate modification is unprecedented among known ribozymes and points to unique features of transition state geometry in the RNase P RNA-catalyzed reaction. 相似文献
10.
Microfluidic-based biosensors toward point-of-care detection of nucleic acids and proteins 总被引:1,自引:1,他引:0
Seokheun Choi Michael Goryll Lai Yi Mandy Sin Pak Kin Wong Junseok Chae 《Microfluidics and nanofluidics》2011,10(2):231-247
This article reviews state-of-the-art microfluidic biosensors of nucleic acids and proteins for point-of-care (POC) diagnostics.
Microfluidics is capable of analyzing small sample volumes (10−9–10−18 l) and minimizing costly reagent consumption as well as automating sample preparation and reducing processing time. The merger
of microfluidics and advanced biosensor technologies offers new promises for POC diagnostics, including high-throughput analysis,
portability and disposability. However, this merger also imposes technological challenges on biosensors, such as high sensitivity
and selectivity requirements with sample volumes orders of magnitude smaller than those of conventional practices, false response
errors due to non-specific adsorption, and integrability with other necessary modules. There have been many prior review articles
on microfluidic-based biosensors, and this review focuses on the recent progress in last 5 years. Herein, we review general
technologies of DNA and protein biosensors. Then, recent advances on the coupling of the biosensors to microfluidics are highlighted.
Finally, we discuss the key challenges and potential solutions for transforming microfluidic biosensors into POC diagnostic
applications. 相似文献