A stereoselective cobalt-containing nitrile hydratase

Nitrile hydratase from Pseudomonas putida NRRL-18668 has been purified and characterized. The purified enzyme catalyzes the hydration of 2(S)-(4'-chlorophenyl)-3-methylbutyronitrile at least fifty times faster than that of 2(R)-(4'-chlorophenyl)-3-methylbutyronitrile. This enzyme is a member of the class of nitrile hydratase that contains cobalt. Visible absorption and CD spectra suggest the cobalt exists as a non-corrin low-spin Co3+ ion in a tetragonally-distorted octahedral ligand field. Chemical reduction of the native enzyme results in a species with the EPR signature of a low-spin Co2+ complex. Like the other cobalt-containing nitrile hydratases, this enzyme is relatively stable, maintaining its activity below 35 degrees C, and it shows a broad activity optimum between pH 7.2 and 7.8. The structural genes for this enzyme have been cloned and sequenced. The deduced amino acid sequences for the alpha and beta subunits show 48-63% and 35-41% homology, respectively, to other sequenced nitrile hydratases. In particular, the cysteine residues in the alpha subunit that have been suggested to coordinate the metal ion in the iron-containing nitrile hydratases [Brennan, B. A., Cummings, J. G., Chase, D. B., Turner, I. M., Jr., & Nelson, M. J. (1996) Biochemistry 35, 10068-10077] are conserved in this enzyme, suggesting that this nitrile hydratase, like the enzyme from Rhodococcus rhodochrous J1, is a member of a newly described class of metalloenzymes with Co3+-thiolate ligation [Brennan, B. A., Alms, G., Nelson, M. J., Durney, L. T., & Scarrow, R. C. (1996) J. Am. Chem. Soc. 118, 9194-9195].     

Analysis of an interferometric optical fibre detection technique applied to silicon vibrating sensors

A simple and accurate analysis of a single-mode optical fibre interferometric displacement detection technique is resented. This analysis is in good agreement with experimental measurements, and shows that angular alignment between the fibre and the sensor is the most critical parameter.     

Clustering based on median and closest string via rank distance with applications on DNA

This paper aims to present several clustering methods based on rank distance. Rank distance has applications in many different fields such as computational linguistics, biology and computer science. The K-means algorithm represents each cluster by a single mean vector. The mean vector is computed with respect to a distance measure. Two K-means algorithms based on rank distance are described in this paper. Hierarchical clustering builds models based on distance connectivity. This paper describes two hierarchical clustering techniques that use rank distance. Experiments using mitochondrial DNA sequences extracted from several mammals are performed to compare the results of the clustering methods. Results demonstrate the clustering performance and the utility of the proposed algorithms.     

dc Electrical Degradation of Perovskite-Type Titanates: I, Ceramics

The rate of the resistance degradation of doped SrTiO₃ ceramics is investigated as a function of various external and material parameters. The effects of the mutually interrelated parameters dc voltage, dc electric field, and thickness of the dielectric are described by power laws. Electron microscopic potential contrast studies show a Maxwell-Wagner polarization leading to a concentration of the electric field at the grain boundaries during the degradation. Based on this finding, the voltage step per grain boundary, ΔΘ_gb, is introduced as a rate-determining parameter which allows an explanation of the influence of the grain size on the degradation rate as well as the difference in the power laws for ceramic and single-crystal samples.     

Optimization of drilling resistance measurement (DRM) user-controlled variables

Drilling resistance measurement (DRM) is recognised as an important on-site micro-invasive procedure for assessment of construction materials. This paper presents a detailed investigation of user-controlled variables and their influence on drilling resistance. The study proves that the ratio of penetration rate/rotational speed is proportional to drilling resistance. Data from Bath stone and an artificial reference stone demonstrates how different materials can be compared using their intrinsic specific energy. It is also shown that adjusting drilling settings does not significantly change drilling measurement variability. However, settings producing high drilling resistance can significantly contribute to drill bit wear. A theoretical framework in which tests can be optimised without compromising the ability to compare data is presented. The framework is of high significance to the conservation industry and will promote a more effective use of DRM. DRM is a minimally invasive procedure particularly appropriate for sensitive heritage structures. Its use can provide the essential mechanical property data required for evaluation of surface consolidation products and specification of repair materials.     

Complexes With Biologically Active Ligands. Part 6 Ni(II) Coordination Compounds of Hydrazine and Heterocyclic Sulfonamides as Inhibitors of the Zinc Enzyme Carbonic Anhydrase

Ternary Ni(II) complexes of hydrazine and eight heterocyclic sulfonamides possessing carbonic anhydrase (CA) inhibitory properties, were prepared and characterized by elemental analysis, spectroscopic, magnetic, thermogravimetric, and conductimetric measurements. The complexes behave as strong inhibitors for two isozymes (I and II) of carbonic anhydrase.     

Generalised Stream X-Machines with Output Delimited Type

Some conditions relating to the automata involved in the W-testing method are discussed. It is also shown how to use the method for reduced automata instead of minimal automata. New design test conditions (weak output distinguishable, strong test-complete and output delimited type) are considered for the generalised stream X-machines (stream X-machines with basic functions replaced by relations and having as output strings of symbols rather than single symbols). It is proved that testing methods similar to those already developed for ordinary deterministic stream X-machines may be applied for generalised stream X-machines with output delimited types. A particular case of generalised stream X-machine with output delimited type is the X-machine with output delimiter, which produces outputs having a distinct right end character. Received October 2000 / Accepted in revised form January 2001     

Contactless Spin Switch Sensing by Chemo-Electric Gating of Graphene

Direct electrical probing of molecular materials is often impaired by their insulating nature. Here, graphene is interfaced with single crystals of a molecular spin crossover complex, [Fe(bapbpy)(NCS)₂], to electrically detect phase transitions in the molecular crystal through the variation of graphene resistance. Contactless sensing is achieved by separating the crystal from graphene with an insulating polymer spacer. Next to mechanical effects, which influence the conductivity of the graphene sheet but can be minimized by using a thicker spacer, a Dirac point shift in graphene is observed experimentally upon spin crossover. As confirmed by computational modeling, this Dirac point shift is due to the phase-dependent electrostatic potential generated by the crystal inside the graphene sheet. This effect, named as chemo-electric gating, suggests that molecular materials may serve as substrates for designing graphene-based electronic devices. Chemo-electric gating, thus, opens up new possibilities to electrically probe chemical and physical processes in molecular materials in a contactless fashion, from a large distance, which can enhance their use in technological applications, for example, as sensors.