Drug Onset Cues, Conditioned Withdrawal, and Drug Relapse: Comment on McDonald and Siegel (2004).

Previous research has shown that under certain conditions environmental cues associated with morphine administration induce drug-opposite conditioned effects that mimic symptoms of opiate withdrawal. R. V. McDonald and S. Siegel (see record 2004-10475-001) extend these observations by demonstrating that acute exposure to a low dose of morphine induces symptoms of opiate withdrawal in rats previously exposed to a high dose of morphine. They hypothesized that early drug onset cues, repeatedly paired with later, larger drug effects, mediate the paradoxical effect of the low drug dose on behavior. They also hypothesized that conditioned withdrawal symptoms induced by the early drug onset cues may mediate the "priming" effect of drugs on relapse and craving. The authors of this comment discuss the degree to which the literature supports this hypothesis. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Construction experiences with SSC collider dipole magnets atFermilab

Full length and short model SSC (Superconducting Super Collider) 50 mm bore dipoles are being built and tested at Fermilab. The mechanical design of these magnets has been determined from experience with the construction of previous superconducting magnets. Construction experience includes coil fabrication, ground insulation, instrumentation, collaring, and yoke assembly. Fabrication techniques are explained, and construction problems and their solutions are reviewed. The relationship of short to long model construction is discussed     

Influences of unconfined states on the optical properties ofquantum-well structures

The population of the unconfined states, with energies above the band edge of the barrier layers, can be significant in some regions of the active volume in high power lasers and amplifiers. This paper analyzes the influences of these states on optical properties, such as gain, refractive index, differential gain, and linewidth enhancement factor, for different quantum-well (QW) structures. Our results show that at high excitation levels, the unconfined band contributions to the real part of the optical susceptibility can be significant, especially in structures with weak quantum confinement potentials. This is in agreement with recent measurements of peak gain and carrier-induced refractive index change versus carrier density, for InGaAs-GaAs QW laser structures     

Simplified method for the construction of an orthonormal base forCPFSK signals

The authors present a simplified method to derive a 2M dimensional orthonormal base for M-ary CPFSK signals based on the well-known Gram-Schmidt procedure. On the basis of this method, the simulated bit error performance of 4, 8 and 16-ary CPFSK modulation in additive white Gaussian noise channels is presented     

Observation of sustained self-pulsation in CW operated flaredY-coupled laser arrays

The time evolution of the near-field radiation patterns of CW operated inphase locked flared `Y' coupled diode laser arrays has been observed with a streak camera. The arrays exhibit sustained self-pulsation at frequencies as high as 4 GHz     

On nested convolutional codes and their application to woven codes

Nested convolutional codes are a set of convolutional codes that is derived from a given generator matrix. The structural properties of nested convolutional codes and nested generator matrices are studied. A method to construct the set of all minimal (rational) generator matrices of a given convolutional code is presented. As an example, two different sets of nested convolutional codes are derived from two equivalent minimal generator matrices. The significant difference in their free-distance profiles emphasizes the importance of being careful when selecting the generator matrices that determine the nested convolutional codes. As an application of nested convolutional codes, woven codes with outer warp, and inner nested convolutional codes are considered. The free-distance profile of the inner generator matrix is shown to be an important design tool.     

An improved decoding algorithm for finite-geometry LDPC codes

In this letter, an improved bit-flipping decoding algorithm for high-rate finite-geometry low-density parity-check (FG-LDPC) codes is proposed. Both improvement in performance and reduction in decoding delay are observed by flipping multiple bits in each iteration. Our studies show that the proposed algorithm achieves an appealing tradeoff between performance and complexity for FG-LDPC codes.     

Enhanced Osteoblast Adhesion to Epoxide‐Functionalized Surfaces

As an alternative to expensive extracellular matrix (ECM) proteins generally applied as coatings in Petri dishes used for cell binding, an innovative system based on epoxide‐functionalized monolayers capable of protein binding is proposed. Since cells bind to material surfaces through proteins, protein‐binding surfaces should also promote cell binding. Here we investigate how the cell‐binding properties of an epoxide‐functionalized surface compares with ECM protein gel coated surfaces and tissue culture polystyrene control surfaces. Glass surfaces are functionalized with glycidoxypropyltriethoxysilane (GOPS), which results in an epoxide‐functionalized surface capable of binding proteins through an epoxide–amine reaction. Advancing contact angle measurements and atomic force microscopy measurements confirm the formation of a homogeneous GOPS monolayer. This monolayer is micropatterned with fluorescein‐labeled ECM protein gel by microcontact printing (µCP). Confocal laser scanning microscopy (CLSM) shows accurately transferred ECM protein gel micropatterns. Osteoblasts that are seeded on these micropatterned substrates show a clear preference for adhering to the epoxide‐functionalized areas. The morphology of these cultured osteoblasts is needle‐like with high aspect ratios. As controls, osteoblasts are cultured on GOPS‐functionalized surfaces, unstructured ECM protein gel surfaces, and tissue culture polystyrene (TCPS). The GOPS surfaces demonstrate a drastic increase in cell adhesion after 2 h, whilst the other tests show no adverse effects of this surface on the osteoblasts as compared to ECM and TCPS. CLSM shows healthy cell morphologies on each surface. It is demonstrated for the first time that epoxide groups outperform ECM protein gel in cell adhesion, thereby providing new routes for cost‐effective coatings that improve biocompatibility as well as exciting, new methodologies to control and direct cell adhesion.     

Selectively Promoting or Preventing Osteoblast Growth on Titanium Functionalized with Polyelectrolyte Multilayers

Titanium plays an important role in medical applications, such as hip joint implants or fixation plates. These implants must perform differently depending on their clinical application. In particular, the osseointegrative properties required of the implant vary with clinical application. The present work is aimed at the functionalization of titanium surface using polyelectrolyte multilayers consisting of natural biopolymers and testing their cell adhesive properties with respect to the osseointegration capacity. Multilayered coatings were created from chitosan (Chi), hyaluronic acid (HA), and gelatine (Gel) through layer‐by‐layer deposition. Cell adhesion, proliferation, and viability were tested in vitro with the human osteoblast cell line CAL‐72 at timescales up to 7 d. Two multilayer coatings consisting of alternated chitosan/gelatin or chitosan/hyaluronic acid layers with the outmost layer of gelatin (Chi/Gel) or hyaluronic acid (Chi/HA), respectively, were tested. The experimental results showed that surfaces functionalized with Chi/Gel and Chi/HA multilayers demonstrated a good initial adhesion of osteoblasts. After 4 d culturing, osteoblast cells were almost completely detached from the substrates functionalized with Chi/HA multilayers. In contrast to Chi/HA, the proliferation of osteoblasts on substrates with Chi/Gel multilayer coatings was statistically significantly higher compared to the control titanium. We have shown that the growth of osteoblasts can be enhanced or completely prevented on a titanium surface functionalized with polyelectrolyte multilayers consisting of natural biopolymers, as desired. Both multilayer coatings, Chi/Gel and Chi/HA, have potential for applications in the field of titanium implants, where rapid osseointegration is essential, and/or where no ingrowth of the implant is desired, respectively.