Pull-Down of Metalloproteins in Their Native States by Using Desthiobiotin-Based Probes

One-third of all proteins are estimated to require metals for structural stability and/or catalytic activity. Desthiobiotin probes containing metal binding groups can be used to capture metalloproteins with exposed active-site metals under mild conditions so as to prevent changes in metallation state. The proof-of-concept was demonstrated with carbonic anhydrase (CA), an open active site, Zn²⁺-containing protein. CA was targeted by using sulfonamide derivatives. Linkers of various lengths and structures were screened to determine the optimal structure for capture of the native protein. The optimized probes could selectively pull down CA from red blood cell lysate and other protein mixtures. Pull-down of differently metallated CAs was also investigated.     

The social consequences of expressive suppression.

At times, people keep their emotions from showing during social interactions. The authors' analysis suggests that such expressive suppression should disrupt communication and increase stress levels. To test this hypothesis, the authors conducted 2 studies in which unacquainted pairs of women discussed an upsetting topic. In Study 1, one member of each pair was randomly assigned to (a) suppress her emotional behavior, (b) respond naturally, or (c) cognitively reappraise in a way that reduced emotional responding. Suppression alone disrupted communication and magnified blood pressure responses in the suppressors' partners. In Study 2, suppression had a negative impact on the regulators' emotional experience and increased blood pressure in both regulators and their partners. Suppression also reduced rapport and inhibited relationship formation. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Sheldon H. White (1928-2005).

Sheldon "Shep" Harold White, one of developmental psychology's most distinguished scholars, died on March 17, 2005, at the age of 76. He is perhaps best remembered for his sharp intellect, generous spirit, good humor, and warm compassion. Shep's principal work lay in three intermeshed areas: children's learning and cognitive development, social programs and policies, and the history of developmental psychology. Shep White is sorely missed by his family and friends, and countless children have unknowingly benefited from his research, scholarship, and contributions to public policy. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Collaborative cross-checking to enhance resilience

Resilience, the ability to adapt or absorb disturbance, disruption, and change, may be increased by team processes in a complex, socio-technical system. In particular, collaborative cross-checking is a strategy where at least two individuals or groups with different perspectives examine the others’ assumptions and/or actions to assess validity or accuracy. With this strategy, erroneous assessments or actions can be detected quickly enough to mitigate or eliminate negative consequences. In this paper, we seek to add to the understanding of the elements that are needed in effective cross-checking and the limitations of the strategy. We define collaborative cross-checking, describe in detail three healthcare incidents where collaborative cross-checks played a key role, and discuss the implications of emerging patterns.     

Understanding of the concept of numerically "less" by bottlenose dolphins (Tursiops truncatus).

In 2 experiments, bottlenose dolphins (Tursiops truncatus) judged the ordinal relationship between novel numerosities. The dolphins were first trained to choose the exemplar with the fewer number of items when presented with just a few specific comparisons (e.g., 2 vs. 6, 1 vs. 3, and 3 vs. 7). Generalization of this rule was then tested by presenting the dolphins with all possible pairwise comparisons between 1 and 8. The dolphins chose the exemplar with the fewer number of items at levels far above chance, showing that they could recognize and represent numerosities on an ordinal scale. Their pattern of errors was consistent with the idea of an underlying analog magnitude representation. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Describing metal surfaces and nanostructures with orbital-free density functional theory

Orbital-free density functional theory (OF-DFT) can be made to scale linearly with sample size, allowing thousands of atoms to be treated explicitly with quantum mechanics. State-of-the-art kinetic energy density functionals and ion–electron pseudopotentials are used to obtain accurate structural property predictions for nanoparticles, nanowires, extended surfaces, and nanoindentation of simple metals.     

New chemical structural features of coal. The structure of coal products

The object of this paper is to demonstrate the usefulness of the transalkylation reaction for determining chemical structural features of products derived from coal. For example, the method is applied to derivatized coals, coals modified by thermolysis, and materials physically or chemically separated from coals. Data are presented for the pyridine soluble and insoluble parts of two coals, an O-methylated coal, a Birch reduced coal, a solvent refined coal, and a coke. Similarities and differences in chemical make-up of these materials are discussed.     

Socially constructing sexuality: Toward a postmodernist theory of sexual intimacy.

Takes issue with the biological focus of traditional theories of sexuality, drawing on postmodern trends such as hermeneutics and social constructionism. It is argued that traditional theories are characterized by a reductionism that precludes meaningfulness and an individualism that precludes genuine intimacy. The theories thus offer an essentially barren account of what is otherwise a richly meaningful human activity. A more fruitful account of human sexuality begins with acknowledging that sexuality is a fundamentally social and meaningful phenomenon and that it can only be understood in the social, historical, and moral context in which human beings live and act. If the linguistic aspect of sexuality were at the heart of theories, biological information could be given its proper place as context for understanding. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Chidamber and Kemerer's metrics suite: a measurement theoryperspective

The metrics suite for object-oriented design put forward by Chidamber and Kemerer (1994) is partly evaluated by applying principles of measurement theory. Using the object coupling measure (CBO) as an example, it is shown that failing to establish a sound empirical relation system can lead to deficiencies of software metrics. Similarly, for the object-oriented cohesion measure (LCOM) it is pointed out that the issue of empirically testing the representation condition must not be ignored, even if other validation principles are carefully obeyed. As a by-product, an alternative formulation for LCOM is proposed