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1.
Functionalized latexes coated by antibodies are used in diagnostic tests for the detection of antigens in biological fluids. A simple kinetic model is presented which is related to the optical monitoring of the formation of specific complexes between antigen and antibody amplified by latex beads. The antibodies are chemically coupled onto chloromethylstyrene (CMST) particles. The kinetic model is able to describe the immunoprecipitin curves of immunolatex beads. The number of fitting parameters is relatively reduced (only three), and the meaning of these parameters can be interpreted in terms of the chemical equilibrium constant, the percentage of active IgG on the latex beads, and optical response. The model explains very well the optical response of immunolatex prepared by covalent coupling of antibodies on polymer carriers. 相似文献
2.
Bernhard Beckermann Jorge Bustamante Reinaldo Martínez-Cruz José M. Quesada 《Calcolo》2014,51(2):319-328
For a given $\theta \in (a,b)$ , we investigate the question whether there exists a positive quadrature formula with maximal degree of precision which has the prescribed abscissa $\theta $ plus possibly $a$ and/or $b$ , the endpoints of the interval of integration. This study relies on recent results on the location of roots of quasi-orthogonal polynomials. The above positive quadrature formulae are useful in studying problems in one-sided polynomial $L_1$ approximation. 相似文献
3.
Jang R Karwowski W Quesada PM Rodrick D Sherehiy B Cronin SN Layer JK 《Ergonomics》2007,50(11):1835-1855
A field study was conducted to investigate spinal kinematics and loading in the nursing profession using objective and subjective measurements of selected nursing tasks observed in a hospital setting. Spinal loading was estimated using trunk motion dynamics measured by the lumbar motion monitor (LMM) and lower back compressive and shear forces were estimated using the three-dimensional (3D) Static Strength Prediction Program. Subjective measures included the rate of perceived physical effort and the perceived risk of low back pain. A multiple logistic regression model, reported in the literature for predicting low back injury based on defined risk groups, was tested. The study results concluded that the major risk factors for low back injury in nurses were the weight of patients handled, trunk moment, and trunk axial rotation. The activities that required long time exposure to awkward postures were perceived by nurses as a high physical effort. This study also concluded that self-reported perceived exertion could be used as a tool to identify nursing activities with a high risk of low-back injury. 相似文献
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6.
Inspired by recent work of Meduna on deep pushdown automata, we consider the computational power of a class of basic program
schemes, NPSDS
s
, based around assignments, while-loops and non-deterministic guessing but with access to a deep pushdown stack which, apart
from having the usual push and pop instructions, also has deep-push instructions which allow elements to be pushed to stack
locations deep within the stack. We syntactically define sub-classes of NPSDS
s
by restricting the occurrences of pops, pushes and deep-pushes and capture the complexity classes NP and PSPACE. Furthermore, we show that all problems accepted by program schemes of NPSDS
s
are in EXPTIME. 相似文献
7.
C. Quesada D. González I. Alfaro E. Cueto A. Huerta F. Chinesta 《The Visual computer》2016,32(11):1465-1479
In this paper, we present the basics of a novel methodology for the development of simulation-based and augmented learning tools in the context of applied science and engineering. It is based on the extensive use of model order reduction, and particularly, of the so-called proper generalized decomposition method. This method provides a sort of meta-modeling tool without the need for prior computer experiments that allows the user to obtain real-time response in the solution of complex engineering or physical problems. This real-time capability also allows for its implementation in deployed, touch screen, handheld devices or even to be immersed into electronic textbooks. We explore here the basics of the proposed methodology and give examples on a few challenging applications never until now explored, up to our knowledge. 相似文献
8.
Crack initiation at corners, V-notches and other situations such as interfaces breaking a free surface (delamination initiation) cannot be correctly predicted by the usual brittle fracture criteria (either Griffith or maximum stress). They give contradictory results and neither one nor the other agrees with the experiments. An additional characteristic length is required to define a satisfying criterion giving rise to the so-called “Finite fracture mechanics”. The crack is supposed to jump this length which depends both on the material properties and the local geometry of the structure; it is not a material parameter. In most cases this crack increment is small. The size effect arises with the interaction between the crack increment and another length characterising a microstructure such as a pore diameter, a notch root radius or an interface layer thickness. The remote load at failure depends on the actual value of this microstructure parameter whereas it was not expected in all cases. Assuming that the two interacting lengths remain small compared to the size of the global structure, an asymptotic procedure allows bringing into evidence the change in the apparent resistance of the structure due to this phenomenon. Results are compared with experiments in various domains: polymers, ceramics and rocks. 相似文献
9.
José-Ceferino Ortega Alejandro Álvarez-Melcón Fernando D. Quesada 《Applied Artificial Intelligence》2013,27(5):323-350
The design of coupled resonator filters used in many telecommunication applications poses an optimization problem that can be tackled with heuristic methods. In many configurations, simple heuristic methods do not give satisfactory results, and the combination in hybrid metaheuristics of local and global search methods is a better approach. This article analyzes the systematic development of hybrid metaheuristic methods for the design of coupled resonator filters. Engineers normally use the MATLAB computing environment to work on the design of these devices, so the available MATLAB optimization toolboxes are used here as a basis to address those optimization problems. The results obtained are in general satisfactory, and the best results are obtained in the experiments with memetic algorithms in which methods based in populations (Genetic Algorithms and Scatter Search) are combined with local search methods to improve individuals in the population at different parts of the metaheuristic. 相似文献