Fine structure of the raphe nuclei of sheep and goats]

The medullary raphe system of sheep and goat can be divided in the solitary nuclei raphe magnus and pallidus besides the impaired Nucleus raphe paramedianus. The nucleus raphe magnus begins in the caudal end of the medulla oblongata running far to the pons. On the other hand you can follow the nucleus raphe pallidus and paramedianus only in distinct area near the obex. Different types of neurons are isolated and described in light- and electronmicroscopical investigations. All three nuclei are poor of synapses.     

Bicriteria robotic operation allocation in a flexible manufacturing cell

Consider a manufacturing cell of two identical CNC machines and a material handling robot. Identical parts requesting the completion of a number of operations are to be produced in a cyclic scheduling environment through a flow shop type setting. The existing studies in the literature overlook the flexibility of the CNC machines by assuming that both the allocation of the operations to the machines as well as their respective processing times are fixed. Consequently, the provided results may be either suboptimal or valid under unnecessarily limiting assumptions for a flexible manufacturing cell. The allocations of the operations to the two machines and the processing time of an operation on a machine can be changed by altering the machining conditions of that machine such as the speed and the feed rate in a CNC turning machine. Such flexibilities constitute the point of origin of the current study. The allocation of the operations to the machines and the machining conditions of the machines affect the processing times which, in turn, affect the cycle time. On the other hand, the machining conditions also affect the manufacturing cost. This study is the first to consider a bicriteria model which determines the allocation of the operations to the machines, the processing times of the operations on the machines, and the robot move sequence that jointly minimize the cycle time and the total manufacturing cost. We provide algorithms for the two 1-unit cycles and test their efficiency in terms of the solution quality and the computation time by a wide range of experiments on varying design parameters.     

Throughput optimization in two-machine flowshops with flexible operations

In this study, a two-machine flowshop producing identical parts is considered. Each of the identical parts is assumed to require a number of manufacturing operations, and the machines are assumed to be flexible enough to perform different operations. Due to economical or technological constraints, some specific operations are preassigned to one of the machines. The remaining operations, called flexible operations, can be performed on either one of the machines, so that the same flexible operation can be performed on different machines for different parts. The problem is to determine the assignment of the flexible operations to the machines for each part, with the objective of maximizing the throughput rate. We consider various cases regarding the number of parts to be produced and the capacity of the buffer between the machines. We present solution methods for each variant of the problem.     

A gantry-based tri-modality system for bioluminescence tomography

A gantry-based tri-modality system that combines bioluminescence (BLT), diffuse optical (DOT), and x-ray computed tomography (XCT) into the same setting is presented here. The purpose of this system is to perform bioluminescence tomography using a multi-modality imaging approach. As parts of this hybrid system, XCT and DOT provide anatomical information and background optical property maps. This structural and functional a priori information is used to guide and restrain bioluminescence reconstruction algorithm and ultimately improve the BLT results. The performance of the combined system is evaluated using multi-modality phantoms. In particular, a cylindrical heterogeneous multi-modality phantom that contains regions with higher optical absorption and x-ray attenuation is constructed. We showed that a 1.5 mm diameter bioluminescence inclusion can be localized accurately with the functional a priori information while its source strength can be recovered more accurately using both structural and the functional a priori information.     

Robust model updating with insufficient data

The increasing need of many industrial fields for highly accurate predictions of performance and reliability gives rise to the need for enhanced underlying mathematical models. The thereby available test data are usually rather limited due to the high costs of experimental measurements. Therefore, decisions have to be made based on limited, incomplete information, which poses a challenging problem.Recently, an approach for coping with insufficient data has been introduced that attempts to extract the information delivered by the data and processes it using few additional assumptions. The underlying distribution is based on an appropriate confidence level providing a safeguard against severe underestimation of the variability of the measured quantities. This method has been applied within the field of statics involving the stochastic identification of one single structural parameter. The present paper shows the extension of this approach to the field of dynamics. It is shown how to deal with insufficient information by applying kernel densities on the stochastic representation of modal data. In addition, the problem of correlation of the established multi-dimensional probability density function will be addressed. As a numerical example the structural dynamics application of the Validation Challenge Workshop has been chosen.     

Role of endoproteolytic dibasic proprotein processing in maturation of secretory proteins in Trichoderma reesei

Cell extracts of Trichoderma reesei exhibited dibasic endopeptidase activity toward the carboxylic side of KR, RR, and PR sequences. This activity was stimulated by the presence of Ca2+ ions and localized in vesicles of low bouyant density; it therefore exhibited some similarity to yeast Kex2. Analytical chromatofocusing revealed a single peak of activity. The dibasic endopeptidase activity was strongly and irreversibly inhibited in vitro as well as in vivo by 1 mM p-amidinophenylmethylsulfonyl fluoride (pAPMSF) but not by PMSF at concentrations up to 5 mM. We therefore used pAPMSF to study the role of the dibasic endopeptidase in the secretion of protein by T. reesei. Secretion of xylanase I (proprotein processing sequence -R-R- downward arrow-R- downward arrow-A-) and xylanase II (-K-R- downward arrow-Q-) was strongly inhibited by 1 mM pAPMSF, and a larger, unprocessed enzyme form was detected intracellularly under these conditions. Secretion of cellobiohydrolase II (CBH II; -E-R- downward arrow-Q-) was only slightly inhibited by pAPMSF, and no accumulation of unprocessed precursors was detected. In contrast, secretion of CBH I (-R-A- downward arrow-Q-) was stimulated by pAPMSF addition, and a simultaneous decrease in the concentration of intracellular CBH I was detected. Similar experiments were also carried out with a single heterologous protein, ShBLE, the phleomycin-binding protein from Streptoalloteichus hindustanus, fused to a series of model proprotein-processing sequences downstream of the expression signals of the Aspergillus nidulans gpdA promoter. Consistent with the results obtained with homologous proteins, pAPMSF inhibited the secretion of ShBLE with fusions containing dibasic (RK and KR) target sequences, but it even stimulated secretion in fusions to LR, NHA, and EHA target sequences. Addition of 5 mM PMSF, a nonspecific inhibitor of serine protease, nonspecifically inhibited the secretion of heterologous proteins from fusions bearing the NHA and LR targets. These data point to the existence of different endoproteolytic proprotein processing enzymes in T. reesei and demonstrate that dibasic processing is obligatory for the secretion of the proproteins containing this target.     

Highly-efficient gene transfer with retroviral vectors into human T lymphocytes on fibronectin

Genetically modified lymphocytes have been successfully used for correction of ADA deficiency in children and in controlling graft-versus-host disease (GvHD) after allogeneic bone marrow transplantation. Low transduction efficiencies are, however, limiting for gene therapeutic strategies based on lymphocytes. In this study we compared protocols for highly efficient gene transfer into human T cells using retroviral vector-containing supernatant. We showed that infection of both human primary T cells and CD4+ Jurkat cells is most efficient on the matrix component fibronectin. Transduction was carried out with a retroviral vector encoding both the human intracytoplasmatically truncated low-affinity nerve growth factor receptor (deltaLNGFR) as a gene transfer marker and the Herpes simplex virus thymidine kinase for negative selection. Based on LNGFR expression genetically modified cells were enriched to near purity by magnetic cell sorting (MACS). Enriched cells could be shown to be highly sensitive to ganciclovir.     

Effect of TiO<Subscript>2</Subscript> addition on crystallization and machinability of potassium mica and fluorapatite glass ceramics

In this study, the effect of TiO₂ addition as a nucleating agent on the crystallization and machinability of potassium mica and fluorapatite base glass ceramics were investigated. Glass compositions were prepared and casted at predetermined temperatures. Differential thermal analysis and XRD methods were applied to characterize phase precipitation sequence and identification of phases. Disc and cylindrical shaped samples were prepared to determine microstructural and mechanical properties in terms of microhardness and machinability. FEG-SEM was used to characterize variation of microstructural constituents depending on the amount of nucleating agent. The results indicate that optimum microstructure and machinability can be obtained in machinable glass-ceramic by the addition of 1 wt% TiO₂ for the composition having 3:7 weight ratio of fluorapatite to potassium mica.     

Frequency response of thin film heads with longitudinal andtransverse anisotropy

A transmission surface model (TSM) that analyzes the conduction of flux by rotation and wall motion is reported. It has been used to quantitatively compare the predicted performance of thin-film heads with longitudinal and transverse magnetic anisotropy. The TSM analysis predicts that a transversely oriented type of thin-film head can be operated at much higher frequencies than a longitudinally oriented head. In addition, its group delay dispersion is much less. Finally, its susceptibility to wall pinning is an order of magnitude less. Therefore, it is concluded that the traditional aversion of head designers to the longitudinal orientation is justified for frequencies above several megahertz     

Microstructure and densification of ZrB2–SiC composites prepared by spark plasma sintering

ZrB₂–SiC composites were prepared by spark plasma sintering (SPS) at temperatures of 1800–2100 °C for 180–300 s under a pressure of 20 MPa and at higher temperatures of above 2100 °C without a holding time under 10 MPa. Densification, microstructure and mechanical properties of ZrB₂–SiC composites were investigated. Fully dense ZrB₂–SiC composites containing 20–60 mass% SiC with a relative density of more than 99% were obtained at 2000 and 2100 °C for 180 s. Below 2120 °C, microstructures consisted of equiaxed ZrB₂ grains with a size of 2–5 μm and α-SiC grains with a size of 2–4 μm. Morphological change from equiaxed to elongated α-SiC grains was observed at higher temperatures. Vickers hardness of ZrB₂–SiC composites increased with increasing sintering temperature and SiC content up to 60 mass%, and ZrB₂–SiC composite containing 60 mass% SiC sintered at 2100 °C for 180 s had the highest value of 26.8 GPa. The highest fracture toughness was observed for ZrB₂–SiC composites containing 50 mass% SiC independent of sintering temperatures.