Thickness control in structural optimization via a level set method

In the context of structural optimization via a level-set method we propose a framework to handle geometric constraints related to a notion of local thickness. The local thickness is calculated using the signed distance function to the shape. We formulate global constraints using integral functionals and compute their shape derivatives. We discuss different strategies and possible approximations to handle the geometric constraints. We implement our approach in two and three space dimensions for a model of linearized elasticity. As can be expected, the resulting optimized shapes are strongly dependent on the initial guesses and on the specific treatment of the constraints since, in particular, some topological changes may be prevented by those constraints.     

Shape optimized design of microwave dielectric resonators by level‐set and topology gradient methods

The finite element method is coupled with the topology gradient (TG) and level‐set (LS) methods for optimizing the shape of microwave components using a computer‐aided design model. On the one hand, the LS approach is based on the classical shape derivative; while on the other hand, the TG method is precisely designed for introducing new perturbations in the optimization domain. These two approaches, which consist in minimizing a cost function related to the component behavior, are first described. Regarding given electrical specifications, these techniques are applied to optimize the distribution of ceramic parts of a dual‐mode resonator in order to improve its behavior. The optimized dielectric resonators result in a wide spurious‐free stop band. A comparison between classical and optimized dual mode resonator is presented. Theoretical results are then validated by careful measurements. © 2009 Wiley Periodicals, Inc. Int J RF and Microwave CAE 2010.     

In search of a stable urban regime for Montreal: issues and challenges in metropolitan development

As with many major North American urban areas since the 1960s, Montreal has faced contextual changes that have brought to the fore the question of its development. However, because of its history and the uncertainties linked with Quebec's constitutional future, attempts to stimulate its development have required certain compromises, often with unexpected results. In order to evaluate the performance of coalitions formed with the objective of meeting the challenges associated with urban and metropolitan development, we have drawn upon the concept of urban regimes. Focusing specifically on Montreal, we ask whether it is possible to transpose urban regime analyses that consider both structural and cultural factors specific to local organized forces. Revealing though they might be, these analyses nevertheless possess certain limitations, notably the underestimation of the weight and increasing importance of players and forces occurring on a metropolitan scale. This is particularly true in the case of Montreal.     

EPR investigation of compound I in Proteus mirabilis and bovine liver catalases: formation of porphyrin and tyrosyl radical intermediates

Compound I of Proteus mirabilis and bovine liver catalases (PMC and BLC, respectively) were studied combining EPR spectroscopy and the rapid-mix freeze-quench techniques. Both enzymes, when treated with peroxyacetic acid, form a catalytic intermediate which consists of an oxoferryl porphyrin pi-cation radical. In PMC this intermediate is semistable, and an unexpected reversible equilibrium under pH influence takes place between two forms of compound I with different coupling between the oxoferryl and the porphyrin pi-cation radical. At acid pH, one form has a ferromagnetic character as in Micrococcus luteus compound I. At neutral pH, another form with a much smaller coupling, reminiscent of the horse radish peroxidase compound I, is detected. The approximate midpoint, estimated for these changes in the range 5.3 < pH < 6.0, approaches the pKa value of an histidyl residue. The residues possibly involved in the transformation are discussed in terms of the known structure of PMC compound I. The EPR spectrum of BLC compound I (pH 5.6), obtained in the millisecond time scale (40 ms), also showed a mixture of two forms which, most probably, correspond to two different magnetic exchange interactions, as in the case of PMC. Taken together, the low-temperature electronic absorption and the EPR spectra of BLC compound I formed in the 0.04-15 s range show that the porphyrin pi-cation radical disappears and, instead, a tyrosyl radical is formed. ENDOR experiments confirm our previously estimated hyperfine couplings to the C2,6 and C3,5 ring protons and the beta-methylene protons of the purported tyrosyl radical. Candidates for such a tyrosyl radical are discussed in connection with the possible electron transfer pathways between the heme active site and the NADPH cofactor.     

Adhesion to and invasion of HeLa cells by pathogenic Escherichia coli carrying the afa-3 gene cluster are mediated by the AfaE and AfaD proteins, respectively

The afa-3 gene cluster, expressed by uropathogenic and diarrhea-associated Escherichia coli strains, determines the formation of an afimbrial adhesive sheath composed of the AfaD and AfaE-III adhesins. The adherence to HeLa cells by recombinant HB101 strains producing both or only one of these two adhesins was investigated. Ultrastructural analyses of the interaction and gentamicin protection assays showed adherence to HeLa cells by HB101 producing both the AfaD and AfaE-III proteins and internalization of a subpopulation of the bacteria into the cells. The interactions of HeLa cells either with HB101 mutants producing AfaD or AfaE-III or with polystyrene beads coated with purified His6-tagged AfaD or His6-tagged AfaE-III proteins were studied. These experiments demonstrated that AfaE-III allows binding to HeLa cells and that AfaD mediates the internalization of the adherent bacteria. Ultrastructural analyses of the interaction of His6-AfaD-gold complexes with HeLa cells confirmed that AfaD is able to bind to the HeLa cell surface and indicated that it penetrates the cells via clathrin vesicles. These data demonstrate that the afa gene cluster is unique among bacteria, as alone it encodes both adhesion to and invasion of epithelial cells.     

Complete amino acid sequence of Proteus mirabilis PR catalase. Occurrence of a methionine sulfone in the close proximity of the active site

The catalase of Proteus mirabilis PR, a peroxide-resistant (PR) mutant of Proteus mirabilis, binds strongly NADPH, which is a unique property among known bacterial catalases. The enzyme subunit consists of 484 amino acid residues for a mass of 55,647 daltons. The complete amino acid sequence was resolved through the combination of protein sequencing, mass spectrometry, and nucleotide sequencing of a PCR fragment. The sequence obtained was compared with that of other known catalases. Amino acids of the active site are all conserved as well as essential residues involved in NADPH binding. Among the amino acids interacting with the heme, a methionine sulfone was found at position 53, in place of a valine in most other catalases. The origin of oxidation of this methionine is unknown, but the presence of this modification could change iron accessibility by large substrates or inhibitors. This posttranslational modification was also demonstrated in the wild-type P. mirabilis catalase.     

Determination of doses for ion implantation in garnet films

The effects of doses in implanted magnetic garnets are investigated by means of magnetic modifications of the implanted layer. Experiments performed on materials with various magnetostriction coefficients and with different growth induced anisotropies show that the stresses are proportional to the ion dose. At high dosage levels an elastic-plastic transformation occurs followed by a progressive destruction of the magnetic order. These results make possible the calculation of the dose necessary for a garnet, given its magnetostriction coefficient and its anisotropy energy.     

Bubble propagation by disks formed by ion-implantation

The investigation of implantation conditions for bubble propagation points to the necessity for a minimum dose (1.5 times 10^{16}ions cm²) and a sufficiently thick profile (0.4 μ). The use of flat profiles gives uniform implanted layers and allows values of the maximum defect concentration far away from the amorphization threshold. Unlike in permalloy circuits, bubble stability and bias field margins do not increase with the drive field. The propagation is not critically dependent upon disk diameter and spacing.