Adventitial Tertiary Lymphoid Organs as Potential Source of MicroRNA Biomarkers for Abdominal Aortic Aneurysm

Abdominal aortic aneurysm (AAA) is an inflammatory disease associated with marked changes in the cellular composition of the aortic wall. This study aims to identify microRNA (miRNA) expression in aneurysmal inflammatory cells isolated by laser microdissection from human tissue samples. The distribution of inflammatory cells (neutrophils, B and T lymphocytes, mast cells) was evaluated in human AAA biopsies. We observed in half of the samples that adventitial tertiary lymphoid organs (ATLOs) with a thickness from 0.5 to 2 mm were located exclusively in the adventitia. Out of the 850 miRNA that were screened by microarray in isolated ATLOs (n = 2), 164 miRNAs were detected in ATLOs. The three miRNAs (miR-15a-3p, miR-30a-5p and miR-489-3p) with the highest expression levels were chosen and their expression quantified by RT-PCR in isolated ATLOs (n = 4), M1 (n = 2) and M2 macrophages (n = 2) and entire aneurysmal biopsies (n = 3). Except for the miR-30a-5p, a similar modulation was found in ATLOs and the two subtypes of macrophages. The modulated miRNAs were then evaluated in the plasma of AAA patients for their potential as AAA biomarkers. Our data emphasize the potential of miR-15a-3p and miR-30a-5p as biomarkers of AAA but also as triggers of ATLO evolution. Further investigations will be required to evaluate their targets in order to better understand AAA pathophysiology.     

Performance of PKI-based security mechanisms in mobile ad hoc networks

Security for ad hoc network environments has received a lot of attention as of today. Previous work has mainly been focussing on secure routing, fairness issues, and malicious node detection. However, the issue of introducing and conserving trust relationships has received considerably less attention. In this article, we present a scalable method for the use of public key certificates and their revocation in mobile ad hoc networks (MANETs). With the LKN-ad hoc security framework (LKN-ASF) a certificate management protocol has been introduced, bringing PKI technology to MANETs. In addition a performance analysis of two different revocation approaches for MANETs will be presented.     

Does {charm} need depth? Similarity and levels-of-processing effects in cued recall.

Eich (1985) recently presented a distributed memory model in which the pattern of results used to support the levels-of-processing view of Craik and Lockhart (1972) was modeled by different degrees of similarity between the encoding context and the to-be-recalled item. We report two experiments in which both phonemic and semantic similarity were varied between pairs of words and incidental acquisition (rhyme vs. category judgments) was varied across the same pairs of items. In both experiments the manipulation of the acquisition task produced a difference in cued-recall performance for positive and negative rhyme and category judgments. Recall was better following a category encoding decision than following a rhyme decision. This difference was independent of the effects of similarity, which demonstrated that Eich's (1985) assumptions regarding the effects of similarity are not sufficient to account for the differences resulting from the manner in which subjects encode information. An alternative method of modeling the levels-of-processing effect within the framework of distributed memory models is proposed. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Context-gated knowledge partitioning in categorization.

According to the knowledge partitioning framework, people sometimes master complex tasks by creating multiple independent parcels of partial knowledge. Research has shown that knowledge parcels may contain mutually contradictory information, and that each parcel may be used without regard to knowledge that is demonstrably present in other parcels. This article reports 4 experiments that investigated knowledge partitioning in categorization. When component boundaries of a complex categorization were identified by a context cue, a significant proportion of participants learned partial and independent categorization strategies that were chosen on the basis of context. For those participants, a strategy used in one context was unaffected by knowledge demonstrably present in other contexts, suggesting that knowledge partitioning in categorization can be complete. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Polarization control in a vertical cavity surface emitting laser submitted to optical feedback

We study experimentally and theoretically two polarization effects in a vertical cavity surface emitting laser submitted to optical feedback. In a first experiment, we obtain flips between two linearly polarized laser modes up to a frequency of 50 MHz using an external cavity with a polarizer. In a second experiment, polarization self modulation is demonstrated up to a frequency of 2.6 GHz, using a quarter wave plate instead. Numerical calculations, based on a four levels model for the active medium, show a good agreement with the experiments.     

Developing competency-based methods for near-real-time air combat problem solving assessment

Air combat scenarios present unique problems where the solution is not obvious. An advanced networked simulation environment can be used to train high-level cognitive air combat skills such as problem solving. A problem in the development of principled and construct oriented assessment is related to determining the level of specificity of the assessment. We present a detailed discussion of the definition of critical competencies associated with combat mission problem solving performance, and we describe our approach to develop and implement an embedded performance assessment system that maps outcomes to these competencies. Finally, we discuss the implications for our approach for comprehensive assessment and discuss our goals for an evaluation of the competency-based approach to assessment.     

Morphological responses in thermally conditioned linear low density polyethylene

An octene-modified linear low density polyethylene has been used to examine the mechanisms involved during thermal annealing. Annealing temperatures ranged from 60 to 100°C. Annealing results in crystallinity increments and these respond to two concurrent effects. One involves the segregation from crystalline regions of low molecular weight moieties in the polymer's molecular weight distribution; the other is lamellar thickening, leading to the formation of more highly perfected crystalline domains. In the present polymer, the two effects were found to be in balance at annealing temperatures near 80°C leading to the optimum distribution of crystalline regions in the amorphous portions of the polymer. The effect of thermal conditioning on mechanical properties of the polymer was illustrated in terms of the initial modulus and the polymer's yield strength. The twin mechanisms of molecular fractionation and lamellar thickening were found to influence both of the mechanical property parameters.     

Transport properties of 1,1-difluoroethane (R152a)

Based on reliable. carefully selected data sets. equations for the thermal conductivity and the viscosity of the refrigerant R 112a are presented. They are valid at temperatures from 240 to 440 K, pressures up to 20 MPa. and densities up to 1050 kg · m^–3. including the critical region.