A Model of the Relationship Between Psychosocial Variables and Diurnal Cortisol Rhythm Under Chronic Stress by Using Structural Equations

By using structural equations, we investigated the effect of chronic stress on salivary cortisol rhythm and proposed a causal model of chronic stress by using psychosocial and physiological data. First, 111 healthy workers (48 males, 63 females) completed questionnaires on chronic stress and lifestyle habits. Then, they provided saliva samples and answered questionnaires that were prepared to assess their psychological states 5 times (on waking up and at 10:00, 11:40, 14:00, and 16:00) on workdays. Structural equation modeling (SEM) revealed that chronic stress and longer commuting time resulted in sleep irregularities and this disrupted the cortisol circadian rhythm. This suggests that chronic stress disrupts the cortisol circadian rhythm even in healthy individuals, and sleep regularity mediates the effect of chronic stress on the cortisol rhythm.

     

Structural change in the Brill transition of Nylon m/n (1) Nylon 10/10 and its model compounds

Structural changes in the Brill transitions of Nylon 10/10 and its model compounds have been investigated by carrying out the temperature-dependent measurements of X-ray diffraction and infrared spectra along with the DSC measurement. The crystal structure at room temperature was found to be the so-called α form with the all-trans zigzag methylene segments. When the samples were heated, the infrared progression bands of the methylene segments, which are sensitive to the length of all-trans segmental parts, were found to change their spectral patterns in the transition temperature region: the progression bands decreased in intensity and disappeared above the transition region. At the same time several new bands were observed to appear, which were found to correspond to the progression bands of (CH₂)₇-(CH₂)₅trans-zigzag segments. These spectral changes indicate that the methylene segments were conformationally disordered by an invasion of some gauche bonds and as a result the effective length of trans-zigzag segments became shorter. This conformational disordering was found to occur more remarkably in the methylene segment of NH-(CH₂)₁₀-NH part than the CO-(CH₂)₈-CO part. At the same time the infrared bands of amide groups, in particular the bands sensitive to the twisting angles about the CH₂-amide bonds were found to show the remarkable change, indicating the local conformational change from planar-zigzag to twisted form in the CH₂-amide moiety. The frequency shift of amide A band (NH stretching mode) indicated a weakening of intermolecular hydrogen bonds, which however, did not disappear up to the melting region. From these data combined with the X-ray diffraction data, the structural disordering in the Brill transition phenomena was deduced concretely.     

Conformational disorder in the Brill transition of uniaxially-oriented nylon 10/10 sample investigated through the temperature-dependent measurement of X-ray fiber diagram

X-ray fiber diagram of uniaxially-oriented nylon 10/10 sample has been measured at the various temperatures. The 11th layer line originating from the methylene zigzag sequences became diffuse above the Brill transition region (140-170 °C), while the layer reflections corresponding mainly to the original repeating period of the parent nylon chains were kept almost unchanged. This observation makes us confirm that in the Brill transition region, the conformational disordering occurs in the methylene sequential parts with keeping the repeating period of the whole skeletal chain by still forming the intermolecular hydrogen bonds between the amide groups of the neighboring chains. This conclusion is consistent with the already-reported infrared spectral data. The characteristic X-ray diffraction patterns observed at the various temperatures were reproduced well by a computer simulation technique utilizing the previously-reported molecular dynamics calculation results.     

Haptic cues as a utility to perceive and recognise geometry

Research has been conducted on how to aid blind peoples’ perceptions and cognition of scientific data and, specifically, on how to strengthen their background in mathematics as a means of accomplishing this goal. In search of alternate modes to vision, researchers and practitioners have studied the opportunities of haptics alone and in combination with other modes, such as audio. What is already known, and has motivated research in this area, is that touch and vision might form a common brain representation that is shared between the visual and haptic modalities and through haptics learning is active rather than passive. In spite of extensive research on haptics in the areas of psychology and neuropsychology, recent advances and rare experiences in using haptic technology have not caused a transfer from basic knowledge in the area of haptics to learning applications and practical guidelines on how to develop such applications. Thus motivated, this study investigates different haptic effects, such as free space, magnetic effects and the bounded box when blind people are given the task of recognising and manipulating classes of 3D objects with which they have varying familiarity. In parallel, this study investigates the applicability of Sjöström’s guidelines on haptic applications development and uses his problem classification to capture knowledge from the experiments. The results of this study show that users can easily recognise and manipulate familiar objects, albeit with some assistance. There is an indication that users completed tasks faster and needed less assistance with magnetic effects. However, they were not as satisfied with this mode. While the results of this study show that haptics have the potential to allow students to conceptualise 3D objects, much more work is needed to exploit this technology to the fullest. Objects with higher complexity are difficult for students, and, in their opinion, the virtual objects (as presented) leave much room for improvement. Sjöström’s error taxonomy proved useful, and four of five sub-guidelines tested were confirmed to be useful in this study.     

Accessibility of audio and tactile interfaces for young blind people performing everyday tasks

Increasingly, computers are becoming tools of communication, information exploring and studying for young people, regardless of their abilities. Scientists have been building knowledge on how blind people can substitute hearing or touch for sight or how the combination of senses, i.e., multimodalities, can provide the user with an effective way of exploiting the power of computers. Evaluation of such multimodal user interfaces in the right context, i.e., appropriate users, tasks, tools and environment, is essential to give designers accurate feedback on blind users’ needs. This paper presents a study on how young blind people use computers for everyday tasks with the aids of assistive technologies, aiming to understand what hindrances they encounter when interacting with a computer using individual senses, and what supports them. A common assistive technology is a screen reader, producing output to a speech synthesizer or a Braille display. Those two modes are often used together, but the research studied how visually impaired students interact with computers using either form, i.e., a speech synthesizer or a Braille display. A usability test has been performed to assess blind grade-school students’ ability to carry out common tasks with the help of a computer, including solving mathematical problems, navigating the web, communicating with e-mail and using word processing. During the usability tests, students were allowed to use either auditory mode or tactile mode. Although blind users most commonly use a speech synthesizer (audio), the results indicate that this was not always the most suitable modality. While the effectiveness of the Braille display (tactile user interface) to accomplish certain tasks was similar to that of the audio user interface, the users’ satisfaction rate was higher. The contribution of this work lies in answering two research questions by analysing two modes of interaction (tactile and speech), while carrying out tasks of varying genre, i.e., web searching, collaboration through e-mail, word processing and mathematics. A second contribution of this work is the classification of observations into four categories: usability and accessibility, software fault, cognitive mechanism and learning method. Observations, practical recommendations and open research problems are then presented and discussed. This provides a framework for similar studies in the future. A third contribution of this work is the elaboration of practical recommendations for user interface designers and a research agenda for scientists.     

Morphological changes of Candida albicans induced by micafungin (FK463), a water-soluble echinocandin-like lipopeptide

Micafungin (FK463), a novel water-soluble echinocandin-like lipopeptide, exerted fungicidal action toward growing cells of Candida albicans at concentrations of 0.1 microg ml(-1) or above. The drug at these levels induced osmotically fragile cells and the resulting fungicidal effect was partially reversed when cultures were grown in the presence of an osmotic stabilizer. Candida cells incubated with fungicidal concentrations of micafungin gradually increased in size and/or became deformed. An electron microscopic study of such micafungin-treated cells revealed morphological alterations in the cell wall; deformation in contour, abnormal septum formation and decrease in thickness of the intermediate layer of the cell wall were prominent. In addition, the structure of cell membranes as well as of membranous cytoplasmic organelles was slightly impaired. These data suggest that micafungin principally affects the normal formation of the cell wall in growing Candida cells.     

Dependence of ion selectivity on ordered orientation of neutral carriers in ion-sensing membranes based on thermotropic liquid crystals

Thermotropic liquid-crystalline compounds were applied as membrane materials (membrane solvent and neutral carrier) for neutral carrier-type ion sensors to investigate how the ordered arrangement of neutral carriers affects the property of the resulting ion sensors. Nematic, smectic, and cholesteric liquid-crystalline compounds were used as the membrane solvents and crown ether derivatives with a molecular structure similar to the liquid-crystalline solvent as the K+ neutral carriers. Polarized IR spectroscopy and X-ray diffraction experiments confirmed that the highly ordered arrangement of membrane components was retained in the liquid-crystal-based ion-sensing membranes containing a neutral carrier and a lipophilic salt. The ordered arrangement of neutral carriers in the liquid-crystalline membranes enhanced the ion selectivity significantly, probably due to the efficient cooperation of two adjacent crown ether moieties in the highly ordered and aggregated state.     

Thermal inactivation of immobilized lipase in 1-alcohols

The thermal inactivation of immobilized lipase from Candida antarctica in 1-alcohols with four to eight carbons at various temperatures could be well expressed using a model in which heterogeneity in the susceptibility of the enzyme to the inactivation was assumed. It was found that the enthalpy-entropy compensation held for inactivation in 1-alcohols.     

NR5A1 is required for functional maturation of Sertoli cells during postnatal development

The orphan nuclear receptor steroidogenic factor 1 (NR5A1 (SF-1)) is expressed in both Sertoli and Leydig cells in the testes. This study investigates the postnatal development of the testes of a gonad-specific Nr5a1 knockout (KO) mouse, in which Nr5a1 was specifically inactivated. The KO testes appeared histologically normal from postnatal day 0 (P0) until P7. However, disorganized germ cells, vacuoles, and giant cells appeared by P14 in the seminiferous tubules of KO but not control mice. Expression of NR5A1 and various factors was examined by immunohistochemistry (IHC). The number of NR5A1-positive Sertoli cells in the KO testes was lower compared with controls at all the developmental stages and decreased to nearly undetectable levels by P21. IHC for anti-Müllerian hormone and p27, immature and mature Sertoli cell markers, respectively, indicated a delay in Sertoli cell maturation in the KO testes. The number of Sertoli cell-expressing factors involved in Sertoli cell differentiation including WT1, SOX9, GATA4, and androgen receptor were lower in the KO testes compared with controls. Furthermore, fewer proliferating cell nuclear antigen-positive proliferative germ cells were observed, and the number of TUNEL-labeled cells was significantly higher in the KO testes compared with controls at P14 and P21, indicating impaired spermatogenesis. IHC for CYP11A1 (SCC) indicated the presence of steroidogenic Leydig cells in the interstitium of the KO testes at all stages examined. These results suggest that NR5A1 is essential for Sertoli cell maturation and therefore spermatogenesis, during postnatal testis development.