Long-term working memory in the rat: Effects of hippocampally applied anisomycin.

The extent to which protein synthesis is involved in working memory was investigated with the protein synthesis inhibitor anisomycin (ANI). Male albino and Long-Evans rats were trained to perform accurately on a 12-arm radial maze when delays of 240 min were interposed between Choice 6 and Choice 7. Bilateral hippocampal cannulas were then implanted. Accuracy on Choices 7–22 was studied when ANI (80 μg/μl) or saline was injected either 30 min before Choice 1 or 5–20 min after Choice 6 in Exp I. Pretrial injection of ANI significantly impaired performance following the 240-min delay, whereas ANI injected during the delay had no such effect. In Exps II and III, the ANI-induced amnesia was replicated, and the temporal course of development of the amnesia was determined. Pretrial administration of ANI did not significantly affect retention after a 2-min delay but produced amnesia after delays of 15 min or longer. Data suggest that protein synthesis is important for the formation of temporary memories, provided the retention interval is long enough. It is suggested that working memory includes both short- and long-term components. Protein synthesis appears to be important for formation of the long-term component, but not the short-term component, of working memory. (31 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Anthroposophic perspectives in primary care

Anthroposophically Extended Medicine (AEM) is a truly integrative healing system. AEM represents an expansion, not an alternative to conventional medicine. Its unique understanding of the interplay among physiological, soul and spiritual processes in healing and illness serves to bridge allopathy with naturopathy, homeopathy, functional/nutritional medicine and other healing systems.     

Luminescent CdS quantum dots as selective ion probes

Water-soluble luminescent CdS quantum dots (QDs) capped by polyphosphate, L-cysteine, and thioglycerol were synthesized in aqueous solution. The ligands were found to have a profound effect on the luminesence response of CdS QDs to physiologically important metal cations. Polyphosphate-capped CdS QDs were sensitive to nearly all mono- and divalent cations, showing no ion selectivity. Conversely, thioglycerol-capped CdS QDs were sensitive to only copper and iron ions. Similar concentrations of physiologically relevant cations, such as zinc, sodium, potassium, calcium, and magnesium ions did not affect the luminescence of thioglycerol-capped CdS QDs. On the other hand, L-cysteine-capped CdS QDs were sensitive to zinc ions and insensitive to other physiologically important cations, such as copper, calcium, and magnium ions. To demonstrate the detection capability of these new ion probes, L-cysteine and thioglycerol-capped CdS QDs were used to detect zinc and copper ions in physiological buffer samples. The detection limits were 0.8 microM for zinc (II) and 0.1 microM for copper (II) ions. The emission enhancement of the QDs by zinc (II) is attributed to activation of surface states, whereas the effective reduction of copper (II) to copper (I) may explain the emission decrease of the thioglycerol-capped CdS QDs when charged with copper ions. Unlike organic fluorescent dyes, the thioglycerol-capped luminescent CdS QDs discriminate between copper and zinc ions and are therefore suitable for the analysis of copper ions in biological samples in the presence of physiological concentrations of zinc ions. The interference of iron ions with zinc and copper ion detection is attributed to an inner filter effect, which is eliminated by adding fluoride ions to form the colorless complex FeF6(3-). To the best of our knowledge, this is first use of luminescent semiconductor quantum dots as selective ion probes in aqueous samples.     

Towards a plasma wake-field acceleration-based linear collider

A proposal for a linear collider based on an advanced accelerator scheme, plasma wake-field acceleration in the extremely nonlinear regime, is discussed. In this regime, many of the drawbacks associated with preservation of beam quality during acceleration in plasma are mitigated. The scaling of all beam and wake parameters with respect to plasma wavelength is examined. Experimental progress towards high-gradient acceleration in this scheme is reviewed. We then examine a linear collider based on staging of many modules of plasma wake-field accelerator, all driven by a high average current, pulse compressed, RF photoinjector-fed linac. Issue of beam loading, efficiency, optimized stage length, and power efficiency are discussed. A proof-of-principle experimental test of the staging concept at the Fermilab test facility is discussed.     

Carrier capture and escape times in In/sub 0.35/Ga/sub 0.65/As-GaAs multiquantum-well lasers determined from high-frequency electrical impedance measurements

We present experimental results on the high-frequency electrical impedance of In/sub 0.35/Ga/sub 0.65/As-GaAs multiquantum-well lasers with varied p-doping levels in the active region. The analysis of the data, using a simple three rate equation model, provides information about the dynamical time constants (the carrier lifetime, the effective carrier capture and escape times) under the laser operation conditions. The addition of p-doping increases the carrier escape time at threshold from 0.7 ns, extracted for the undoped devices, up to a value higher than 2 ns for the p-doped lasers. The effective capture time is estimated to be between 2 and 5 ps.     

Inhibition of cerebral protein synthesis does not prolong short-term memory.

In 4 experiments, male Swiss-Webster CD-1 mice were given a single sc injection of a cerebral protein synthesis inhibitor, anisomycin (ANI, 1 mg/S), 20 min prior to a single trial of passive avoidance training. Ss demonstrated impaired retention at test given 3 hrs, 6 hrs, 1 day, and 7 days after training. Retention was not significantly different from that of saline controls when tests were given .5 or 1.5 hrs after training. Prolonging inhibition of brain protein synthesis by giving either 1 or 2 additional injections of ANI at 2 hrs or at 2 and 4 hrs after training did not prolong good retention performance. The temporal development of impaired retention in ANI-treated Ss could not be accounted for by drug dosage, duration of protein synthesis inhibition, or nonspecific sickness at test. In contrast to the suggestion that protein synthesis inhibition prolongs short-term memory, these results indicate that short-term memory is not prolonged by antibiotic drugs that inhibit cerebral protein synthesis. All evidence seems consistent with the hypothesis that short-term memory is independent of protein synthesis and that the establishment of long-term memory depends on protein synthesis during or shortly after training. (33 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Sintering rheology of amorphous polymers

The activation energy for sintering of poly(methyl methacrylate) particle pairs is shown to be similar to their activation energy for Newtonian flow. Sintering progress with time is in good agreement with the Frenkel's coalescence theory. Typical sintering shear rates are shown to be very low and potential energy change (two particles) is small in comparison with the surface energy change. These results lead to the conclusion that the coalescence sintering mechanism of amorphous polymers above their glass transition temperature is essentially a Newtonian viscous flow mechanism where surface tension is the major driving force. A periodical phenomenon associated with sintering progress with time is reported and a supporting mechanism is proposed.     

The recovery of potassium chloride from Dead Sea brines by precipitation and solvent extraction

A process is proposed for the recovery of potassium from brines by precipitation as potassium perchlorate, followed by conversion to potassium chloride by liquid anion exchange with a tertiary amine in the form of its hydrochloride. Regeneration of the amine salt is effected by means of lime and hydrochloric acid. The process was tested on Dead Sea brine, containing 13 g/l KCl. Precipitation at 30 °C gave a 40% yield of potassium, cooling to 0 °C increased the yield to over 80%. The anion exchanger used was the hydrochloride of trioctylamine dissolved in toluene; this performed satisfactorily and phase separation was easy. A separation factor of 130 was obtained for the ClO₄^?/Cl^? separation. Regeneration of the amine was tested with various bases.