Enhancement of nutraceutical properties of licorice callus cultures using sample pre-treatment strategy

Licorice is an herbal plant in the Leguminosae family, and its roots and rhizomes are used as sweeteners in food and confectionery products. Moreover, it has a distinct inflammatory activity. In the present study, a sample pre-treatment method to induce the deglycosylation of active metabolites in callus cultures of Glycyrrhiza inflata (GI) and Glycyrrhiza glabra (GG) was developed. The results of the method evaluation showed the biotransformation of ononin to formononetin, a rare flavonoid found in trace amounts in licorice. The magnitude of enhancement was 3- and 19-fold in the GI and GG samples, respectively. Moreover, the anti-inflammatory activity assay showed that the potency of the sample pre-treatment group was higher than that of the untreated group because it exerted an enhanced suppression of cyclo-oxygenase 2 (COX-2), interleukin-6 (IL-6) and inducible nitric oxide synthase (iNOS) gene expression. This is the first report on the anti-inflammatory activity of licorice callus, which has the potential to be utilised as a functional food for health promotion. These findings support the idea of using sample preparation to impart nutraceutical properties to plant products.     

Monte Carlo simulation of hydrogen absorption in palladium and palladium–silver alloys

A modified Monte Carlo (MC) simulation was performed to investigate the hydrogen absorption behavior in Pd and Pd–Ag alloys of the composition Pd_xAg_1−x (x=0.7–0.8) under H₂ pressure (0.1 MPa) at different temperatures. The present method employed can consider the dissociative adsorption of hydrogen molecule and the subsequent absorption of hydrogen atom by formalizing the relationship between the pressure of hydrogen molecule and hydrogen atom. The potential parameters were determined to reproduce the solution enthalpy of hydrogen in pure metals. The results are in good agreement with experimental findings as well as previous theoretical studies. We confirmed that our method is useful to simulate the absorption of hydrogen in metals and alloys.     

Rates for intramolecular excimer formation of poly(α-methylstyrene) measured by using picosecond pulse radiolysis

Summary The rate constants for intramolecular excimer formation, k_DM, of poly(α-methylstyrene) with different molecular weight were determined by using picosecond pulse radiolysis. Values of k_DM for poly(α-methylstyrene) are a little smaller than those for polystyrene with nearly same molecular weight. It appears to be mainly due to steric hindrance by methyl substituent of main chain.     

A lightweight sensing method of tooth-touch sound for disabled person using remote controller

To support disabled people to use remote controllers, several biological signals are used. The tooth-touch is one of desirable biological signals. This is because it is the simple and natural human behavior. However, a sophisticated signal processing to extract only the tooth-touch sound is needed since the tooth-touch sound is mixed with the voice sound. This paper proposes a lightweight sensing method extracting the tooth-touch without a sophisticated signal processing to eliminate only voice sound from the sound wave in which tooth-touch and voice are mixed. Instead of the audible sound wave, proposal uses a shock wave (i.e., ultrasonic wave) which is generated when the upper tooth and the lower tooth hit each other. Using the shock wave generated to detect the tooth-touch, a trivial high-pass filter can eliminate only voice sound in the lower frequency domain than in the ultrasonic domain including the tooth-touch. Through a preliminary experiment that uses a conventional microphone and well-known digital high-pass filter, we show that the used electret microphone can sense the tooth-touch as ultrasonic wave and the high-pass filter can extract only tooth-touch. Then, we show some robustness of our method by using the sound waves including the voice and tooth-touch. In addition, we design the filtering hardware to implement a small and cheap system-on-chip achieving a real-time operation. Through the implementation of Field Programmable Gate Array, and the simulation, we show that our hardware is small and performs well for a real-time operation.     

Development of reel-to-reel microchip mounting system for fabrication of meter-long LED lighting tapes

We have developed a reel-to-reel microchip mounting system that continuously mounts LED chips and other microchips on meter-long flexible printed circuit board (PCB) tape for 1.2-m-long standard LED light tubes. Mounting microchips on meter-long PCBs is difficult because a large chip mounter is expensive and the chip positioning stage is difficult to move in a meter-wide area with an accuracy of hundreds of micrometers. Hence, we developed a new microchip mounting system that utilizes a small chip mounter and reel winding machines. The system repeatedly moves the long PCB tape by a certain length with the reel winding machines and mounts the chips on it. The PCB tape (which is 5 mm wide) is made by fabricating long PCB tapes which are 25 cm × 26.6 m through a roll-to-roll PCB process and slitting them into meter-long tapes. The reel-to-reel system repeatedly mounts chips by adjusting their positions through image processing of the copper wiring pattern on the PCBs. Our constructed system mounted 24 LED chips with a pitch of 5 cm with an accuracy of 0.082 mm to form a 1.2-m-long LED tape. The luminance of the tape is 12.4 lx at a distance of 1 m, which is the luminance of outdoor corridor lighting. Therefore, this system can be used for meter-long tape lighting.     

Interfacial Reactions between Sapphire and Silver—Copper—Titanium Thin Film Filler Metal

Wetting and brazing studies of sputtering-deposited, submicrometer thin film filler metal in an Ag—Cu—Ti/Al₂O₃ system were performed. The interfacial reaction layer between the filler metal and Al₂O₃ was investigated. It is possible to make a brazing joint even with a reaction layer of less than 100 nm thickness. Different types of interfacial reaction layers were observed when the Ti content in the filler metal was varied. The Cu—Ti—O system compounds were observed in the samples with high wetting capabilities, but not in the sample with low wetting characteristics. It was found that these compounds are substances that promote effective brazing.     

Design of new catalysts for ecological high-quality transportation fuels by combinatorial computational chemistry and tight-binding quantum chemical molecular dynamics approaches

Recently, we introduced a concept of combinatorial chemistry to computational chemistry and proposed a new method called “combinatorial computational chemistry”, which enables us to perform a theoretical high-throughput screening of catalysts. In the present paper, we reviewed our recent application of our combinatorial computational chemistry approach to the design of new catalysts for high-quality transportation fuels. By using our combinatorial computational chemistry techniques, we succeeded to predict new catalysts for methanol synthesis and Fischer–Tropsch synthesis. Moreover, we have succeeded in the development of chemical reaction dynamics simulator based on our original tight-binding quantum chemical molecular dynamics method. This program realizes more than 5000 times acceleration compared to the regular first-principles molecular dynamics method. Electronic- and atomic-level information on the catalytic reaction dynamics at reaction temperatures significantly contributes the catalyst design and development. Hence, we also summarized our recent applications of the above quantum chemical molecular dynamics method to the clarification of the methanol synthesis dynamics in this review.     

Preparation and characterization of natural rubber dispersed in nano-matrix

Preparation of a model nano-matrix-dispersed polymer was investigated in terms of graft-copolymerization of deproteinized natural rubber latex with styrene, using tert-butyl hydroperoxide/tetraethylenepentamine as an initiator. The products were characterized by ¹H-NMR spectroscopy and size-exclusion-chromatography after ozonolysis. The grafting efficiency of styrene was found to be more than 90% under the best condition of the graft-copolymerization. The morphology of the film specimens, prepared from graft-copolymers, was observed by transmission electron microscopy after staining the films with OsO₄. Natural rubber particle of about 0.5 μm in diameter was dispersed in polystyrene matrix of about 15 nm in thickness.     

Effect of fatty acids on the strain‐induced crystallization of natural rubber detected by tear energy measurements

The strain‐induced crystallization of natural rubber (NR) was investigated by the measurement of the tear energy of a crosslinked blend consisting of NR and noncrystalline styrene–butadiene rubber (SBR). When NR was dispersed into the SBR matrix, the tear energy of SBR increased at various temperatures and tear rates. After the application of the principle of time–temperature superposition to the tear energy according to the Williams–Landel–Ferry equation, two distinct curves were found for the NR/SBR blend with respect to the reduced tear rate, despite the fact that the tear energy of SBR or the SBR/SBR blend gave its own single composite curve. When the fatty acid in the NR/SBR blend was removed by acetone extraction, the tear energy of the blend drew a single composite curve. The conversion of the two curves into the single composite curve for the NR/SBR blend suggested that the tear energy depended on the strain‐induced crystallization of NR dispersed in the SBR matrix, which was suppressed by the removal of the fatty acid. © 2005 Wiley Periodicals, Inc. J Appl Polym Sci 98: 613–619, 2005     

Meter-scale large area LED-embedded light fabric for the application of fabric ceilings in rooms

We have developed a meter-scale light emitting diode (LED)-embedded light fabric and its weaving machine for application to a light device for fabric ceilings, which have recently become desired for lightweight safe ceilings in Japan and other countries with frequent earthquakes. The LED fabric structure is 1.2-m-wide woven fabric that has 5-mm-wide LED chip-mounted printed circuit board (PCB) tapes as wefts. LEDs are mounted on the tape of PCBs with a reel-to-reel chip mounting system. Then, the LED-mounted tapes are woven with a developed automatic looming machine that aligns the weft with an accuracy of 0.9 mm, which is suitable for precise arrangement of LEDs and wiring to power supply. A 1.2 × 1.2 m LED-embedded light fabric weighing 320 g/m² was woven. The luminance of the LED fabric is 353 lx at a distance of 1 m, which is the luminance of conventional office lighting. The temperature increase of LEDs without a rigid cooling aluminum plate is only 5.8 °C, and the LED fabric is flexible enough to sustain 1,000 bends down to a radius of 3 mm. This LED fabric and its weaving technology will lead to light devices that have lightweight, large area, and high flexibility for fabric ceilings, walls, and other large areas in homes and offices.