培养细胞透射电镜超薄切片制备方法

应用透射电镜观察培养细胞的超微结构都离不开超薄切片的制备。培养细胞小、数量少以及培养细胞方式的多样化，这些都给样品制备工作带来一定的难度。完全按照文献报道的制备方法，已不能完全满足培养细胞电镜观察的要求。我们根据观察的需要选择细胞的不同培养方式，并根据不同的     

珠海市污水处理厂污泥处理处置探讨

在总结国内外常用污泥处理处置技术的基础上,结合珠海市污泥处理处置现状以及污泥特性,探讨其污泥处理工艺和处置设施厂址选择.建议珠海市中心城区污水处理厂污泥适宜的处置工艺为:半干化填埋和干化焚烧;并针对不同的工艺,对污泥集中处置设施厂址进行了综合比选.     

注凝析油开采稠油的地质工艺

在西西伯利亚俄罗斯油田开展了注凝析油开发稠油的研究，现场实验结果表明，注入25%的凝析油，可使其混合液粘度达到原高粘油粘度的1/60，以俄罗斯油田为例，探讨了注凝析油矿场试验的可行性，预测了凝析油的用量和可能产出的油量，并对原油外输提出了一系列更换工艺设备的要求。     

国内企业承接对外加工业务的几点思考

跨国公司的制造重心转移，给中国的制造厂家带来了大量的外协加工业务，因而对中国的制造厂商来说，不仅仅是挑战，同时也是极好的机遇。     

面向分层移动IPv6的高效快速切换方案

快速切换方案在降低切换延迟方面所取得的效果已经得到了公认,但其交互过程较为复杂,不利于该技术的大规模推广.针对该问题,文中结合分层移动IPv6提出了一种高效快速切换方案.该方案不仅通过缩短重复地址检测的时延进一步降低快速切换的切换延迟,还借助应用层和链路层的信息有选择的执行快速切换,从而既降低了切换延迟也简化了切换交互过程、降低切换注册中的消息流量.实验结果表明,本方案中注册开销和切换延迟均低于现有快速分层移动IPv6方案.     

基于OSEK规范的网络管理的研究与改进

首先论述了基于OSEK规范的网络管理特点并对OSEK网络管理的工作机制进行了详细分析;在基于OSEK网络管理特点和工作机制的基础上,对OSEK网络管理在以太网上的扩展、资源占有率优化和实时性提高几个方面给出了解决方案。     

基于SPW的流星余迹极低信噪比通信系统建模与仿真*

SPW具有丰富的通信库和DSP库,且具有灵活的自定义模块能力,是一种复杂通信系统建模和仿真工具.流星余迹通信是一种特殊的通信方式,在通信领域中具有特殊的重要地位.介绍了流星余迹极低信噪比通信系统原理的基础,给出了基于SPW的流星余迹极低信噪比通信系统仿真模型及仿真结果.     

基于AUKF的分布式电源系统虚假数据攻击检测方法

虚假数据注入攻击(FDIA)是一种典型的网络攻击方式,其通过破坏数据完整性进而误导电力系统状态估计结果,严重危害电网运行安全.随着国家大力发展新能源产业,越来越多的分布式电源注入电力系统,使得电网中大量测量数据具有随机、多变的特性,分布式电源系统中的虚假数据检测难度大大增加.针对这一问题,本文构建了分布式电源系统状态估计模型和FDIA模型,采用了一种基于自适应无迹卡尔曼滤波(AUKF)的检测算法,通过AUKF算法对电网中的状态量进行估计,在此过程中经一致性检验、虚假数据检测并计算相应的阈值,判断系统是否受到攻击.结果表明,当系统中注入攻击强度为[-10,20]dB的虚假数据时,采用该方法均能准确识别虚假数据;当系统中测量值发生突变时,不会被该算法误判为虚假数据注入,避免造成错误的调度选择.     

In vitro comparison of hepatic metabolism of 9cis-11 trans and 10trans-12cis isomers of CLA in the rat

Hepatic metabolism of the two main isomers of CLA (9cis-11 trans, 10trans-12cisC_18∶2) was compared to that of oleic acid (representative of the main plasma FA) in 16 rats by using the in vitro method of incubated liver slices. Liver tissue samples were incubated at 37°C for 17h under an atmosphere of 95% O₂/5%CO₂ in a medium supplemented with 0.75 mM of FA mixture (representative of circulating nonesterified FA) and with 55 μM [1-¹⁴C]9cis-11 trans C_18∶2, [1-¹⁴C]10trans-12cis C_18∶2, or [1-¹⁴C]oleate. The uptake of CLA by hepatocytes was similar for both isomers (9%) and was three times higher (P<0.01) than for oleate (2.6%). The rate of CLA isomer oxidation was two times higher (49 and 40% of incorporated amounts of 9cis-11 trans and 10trans-12 cis, respectively) than that of oleate (P<0.01). Total oxidation of oleate and CLA isomers into [¹⁴CO₂] was low (2 to 7% of total oxidized FA) compared to the partial oxidation (93 to 98%) leading to the production of [¹⁴C] acid-soluble products. CLA isoemrs escaping from catabolism were both highly desaturated (26.7 and 26.8%) into conjugated 18∶3. Oleate and CLA isomers were mainly esterified into neutral lipids (30%). They were slowly secreted as parts of VLDL particles (<0.4% of FA incorporated into cells), the extent of secretion of oleate and of 10trans-12 cis being 2.2-fold higher than that of 9cis-11 trans (P<0.02). In conclusion, this study clearly showed that both CLA isomers were highly catabolized by hepatocytes, reducing their availability for peripheral tissues. Moreover, more than 25% of CLA escaping from catabolism was converted into conjugated 18∶3, the biological properties of which remain to be elucidated.