Recognition of Adulterated Oils by Direct Analysis of the Minor Components

Recognition of adulteration by other oils via direct analysis of the minor components (“sterol fraction”) is shown for olive oil. 10 % of various oils were admixed, the free alcohols silylated and the minor components analyzed by on-line coupled LC-GC-FID. For most oils, even smaller additions can be recognized. Admixed oils can no longer be determined, however, if their minor components have been removed by strong raffination. Bleaching of rapeseed oil with 7% of earth at 180 °C, in fact, completely removed free and esterified sterols.     

A comparison between wet and cryogenic drilling of CFRP/Ti stacks

This paper compares conventional and cryogenic cooling in the deep hole drilling of carbon fiber reinforced polymer (CFRP)/Ti stacks. Various parameters are taken into account to find if the use of cryogenic coolant is justified by the improvement of the final results. Both the thrust and the torque were acquired continuously during the machining operations and compared both in average and distribution. The use of a cryogenic coolant brings a reduction in thrust force and torque without any sensible drawback. Overall the results prove that cryogenic drilling is a suitable technology for CFRP/Ti stack drill.     

Matrix-Based Discrete Event Control for Surveillance Mobile Robotics

This paper focuses on the control system for an autonomous robot for the surveillance of indoor environments. Our approach proposes a matrix-based formalism which allows us to merge in a single framework discrete-event supervisory control, conflict resolution and reactive control. As a consequence, the robot is able to autonomously handle high level tasks as well as low-level behaviors, solving control and decision-making issues simultaneously. Moreover, the matrix-based controller is modular and can be easily reconfigured if mission characteristics or robot hardware configuration change. An illustrative example and a report on experimental investigations are provided to illustrate the main features of the proposed approach.     

New Insights into the Decisional Use of Process Capability Indices via Hypothesis Testing

Process capability indices C_p, C_pk, and C_pm are still nowadays widely used in industry—thanks to their easy formulation and implementation. This paper aims to give new mathematical insights in order to support their use in decision‐making via hypothesis testing. The minimum sample size usually needed in the applications to achieve fixed significance level and power is reported in light of the new mathematical aspects for C_pk and C_pm, which avoid misleading conclusions and the use of extensive numerical experiments. In addition, power curves for C_pk and C_pm, which have not previously appeared in the literature before, are also presented. Lastly, easy‐to‐follow diagrams for hypothesis testing with C_pk and C_pm and two critical scenarios for C_pm are included in the paper to facilitate the applicative use and the comprehension of the novel inferential aspects. Copyright © 2014 John Wiley & Sons, Ltd.     

Sliding-Mode Control With Double Boundary Layer for Robust Compensation of Payload Mass and Friction in Linear Motors

Direct drives with linear motors have been recently attracting the attention of both industry and academia. The main peculiarity of these systems is the lack of mechanical reduction and transmission devices, which makes the influence of some uncertain electromechanical phenomena (e.g., friction, cogging forces, etc.) and load disturbances much more significant than in the case of conventional rotary actuators. This paper describes a control system for a tubular synchronous linear motor based on a sliding-mode control (SMC) and a proportional-integral (PI)-based equivalent disturbance observer. The distinctive peculiarities of the proposed scheme are the use of a control law that guarantees the stability of the system regardless of the payload mass, the adoption of a double boundary layer addressing effectively the harmful effects of static friction, and the introduction of a simple PI-based equivalent disturbance observer that avoids steady-state errors regardless of model uncertainties and external disturbances. The reduced computational cost of the control law, alongside with the introduction of the effective design criteria for the SMC and the disturbance observer, makes the implementation of the proposed approach as simple as standard cascaded linear control schemes using industrial microcontrollers. The aforementioned considerations are validated by extensive experiments.     

Tract‐tracing study of the extrabulbar Olfactory projections in the brain of some teleosts

The extrabulbar olfactory projections (EBOP) is a collection of nerve fibers that originate from primary olfactory receptor neurons. These fibers penetrate into the brain, bypassing the olfactory bulbs (OBs). While the presence of an EBOP has been well established in teleosts, here we morphologically characterize the EBOP structure in four species each with a different morphological relationship of OB with the ventral telencephalic area. Tract‐tracing methods (carbocyanine DiI/DIA and biocytin) were used. FMRFamide immunoreactive nervus terminalis (NT) components were also visualized to define any neuroanatomical relationship between the NT and EBOP. Unilateral DiI/DiA application to the olfactory chamber stained the entire olfactory epithelium, olfactory nerve fibers, and ipsilateral olfactory bulb. Labeled primary olfactory fibers running ventromedially as extrabulbar primary olfactory projections reached various regions of the secondary prosencephalon. Only in Moenkhausia sanctaefilomenae (no olfactory peduncle) did lipophilic tracer‐labeled fibers reach the ipsilateral mesencephalon. The combination of tracing techniques and FMRFamide immunohistochemistry revealed a substantial overlap of the label along the olfactory pathways as well as in the anterior secondary prosencephalon. However, FMRFamide immunoreactivity was never colocalized in the same cellular or fiber component as visualized using tracer molecules. Our results showed a certain uniformity in the neuroanatomy and extension of EBOP in all four species, independent of the pedunculate feature of the OBs. The present study also provided additional evidence to support the view that EBOP and FMRFamide immunoreactive components of the NT are separate anatomical entities. Microsc. Res. Tech. 78:268–276, 2015. © 2015 Wiley Periodicals, Inc.     

Approximate Analytic Estimate of Axial Fluid Conduction in Laminar Forced Convection Tube Flows with Zero-to-Uniform Step Wall Heat Fluxes

The influence of axial fluid conduction on low Péclet number flows in the thermal entrance region of long circular tubes is investigated in this theoretical study. The convective heat transport of viscous fluids relates to a specific condition under which the first part of the tube ( x h 0) is insulated and the second part of the tube ( x > 0) receives a heat flux of uniform intensity. A conjugate one-dimensional lumped model produces a solution of compressed algebraic form that is able to deliver dependable mean bulk temperatures that are in perfect agreement with those obtained numerically by the formal conjugate two-dimensional distributed model. As a by-product of the succession of algebraic calculations within the platform of the lumped model, the critical Péclet number Pe cr has been easily quantified. This number is a figure-of-merit of remarkable importance in the modeling of forced heat convection in tubes because it establishes the threshold between two contrasting situations: one embracing axial fluid conduction (finite Pe) and the other implicating negligible axial fluid conduction (Pe M X ). In addition, the local wall temperatures were calculated with an approximate engineering procedure, showing good agreement with those determined numerically by the formal conjugate two-dimensional distributed model.     

Survey of 1,2-dicarbonyl compounds in commercial honey of different floral origin

In this study we conducted a survey of the concentrations of the major 1,2-dicarbonyl compounds in 40 commercial honey samples from 12 different floral origins. 3-Deoxyglucosone (3-DG), glyoxal (GO), and methylglyoxal (MGO) were measured, using their corresponding quinoxaline derivatives, by high-performance liquid chromatography (HPLC). The analytical performance of the HPLC method for the analysis of 1,2-dicarbonyl compounds was evaluated in terms of linearity, limits of detection (LODs), limits of quantification (LOQs), and precision. Linearity over 2 orders of magnitude, LODs (0.01-0.04 mg/kg), and LOQs (0.03-0.12 mg/kg) were calculated. Instrumental precision, as measured by the repeatability relative standard deviation% (RSDr%), was found to be between 0.22% and 0.55%. Furthermore, the concentrations of factors GO and MGO with respect to 3-DG were also calculated for rapid quantification in honey. In honey samples, the concentrations of 3-DG ranged from 75.9 to 808.6 mg/kg and were significantly higher (up to 100-fold) than those of 5-hydroxymethylfurfural (HMF). Values for GO and MGO were 0.1-10.9 and 0.2-2.9 mg/kg, respectively. The chemical characteristics that most influenced the levels of 1,2-dicarbonyl compounds in honey were found to be pH and total phenols. This was supported by multivariate analysis used to classify different honey types with respect to their chemical characteristics. In addition, both dicarbonyls and phenols are believed to contribute to the development of the final color of honey.     

Post Zygotic,Somatic, Deletion in KERATIN 1 V1 Domain Generates Structural Alteration of the K1/K10 Dimer,Producing a Monolateral Palmar Epidermolytic Nevus

Palmoplantar keratodermas (PPKs) are characterized by thickness of stratum corneum and epidermal hyperkeratosis localized in palms and soles. PPKs can be epidermolytic (EPPK) or non epidermolytic (NEPPK). Specific mutations of keratin 16 (K16) and keratin 1 (K1) have been associated to EPPK, and NEPPK. Cases of mosaicism in PPKs due to somatic keratin mutations have also been described in scientific literature. We evaluated a patient presenting hyperkeratosis localized monolaterally in the right palmar area, characterized by linear yellowish hyperkeratotic lesions following the Blaschko lines. No other relatives of the patient showed any dermatological disease. Light and confocal histological analysis confirmed the presence of epidermolityic hyperkeratosis. Genetic analysis performed demonstrates the heterozygous deletion {"type":"entrez-nucleotide","attrs":{"text":"NM_006121.4","term_id":"1519311739"}}NM_006121.4:r.274_472del for a total of 198 nucleotides, in KRT1 cDNA obtained by a palmar lesional skin biopsy, corresponding to the protein mutation {"type":"entrez-protein","attrs":{"text":"NP_006112.3","term_id":"119395750"}}NP_006112.3:p.Gly71_Gly137del. DNA extracted from peripheral blood lymphocytes did not display the presence of the mutation. These results suggest a somatic mutation causing an alteration in K1 N-terminal variable domain (V1). The deleted sequence involves the ISIS subdomain, containing a lysine residue already described as fundamental for epidermal transglutaminases in the crosslinking of IF cytoskeleton. Moreover, a computational analysis of the wild-type and V1-mutated K1/K10 keratin dimers, suggests an unusual interaction between these keratin filaments. The mutation taster in silico analysis also returned a high probability for a deleterious mutation. These data demonstrate once again the importance of the head domain (V1) of K1 in the formation of a functional keratinocyte cytoskeleton. Moreover, this is a further demonstration of the presence of somatic mutations arising in later stages of the embryogenesis, generating a mosaic phenotype.