Real-time organic aerosol chemical speciation in the indoor environment using extractive electrospray ionization mass spectrometry

Understanding the sources and composition of organic aerosol (OA) in indoor environments requires rapid measurements, since many emissions and processes have short timescales. However, real-time molecular-level OA measurements have not been reported indoors. Here, we present quantitative measurements, at a time resolution of five seconds, of molecular ions corresponding to diverse aerosol-phase species, by applying extractive electrospray ionization mass spectrometry (EESI-MS) to indoor air analysis for the first time, as part of the highly instrumented HOMEChem field study. We demonstrate how the complex spectra of EESI-MS are screened in order to extract chemical information and investigate the possibility of interference from gas-phase semivolatile species. During experiments that simulated the Thanksgiving US holiday meal preparation, EESI-MS quantified multiple species, including fatty acids, carbohydrates, siloxanes, and phthalates. Intercomparisons with Aerosol Mass Spectrometer (AMS) and Scanning Mobility Particle Sizer suggest that EESI-MS quantified a large fraction of OA. Comparisons with FIGAERO-CIMS shows similar signal levels and good correlation, with a range of 100 for the relative sensitivities. Comparisons with SV-TAG for phthalates and with SV-TAG and AMS for total siloxanes also show strong correlation. EESI-MS observations can be used with gas-phase measurements to identify co-emitted gas- and aerosol-phase species, and this is demonstrated using complementary gas-phase PTR-MS observations.     

Diurnal and spatial variation of xylem dielectric constant in Norway Spruce (Picea abies [L.] Karst.) as related to microclimate, xylem sap flow, and xylem chemistry

Spatial and temporal variations in vegetation dielectric properties strongly influence the microwave backscatter characteristics of forested landscapes. This paper examines the relationship between xylem tissue dielectric constant, xylem sap flux density, and xylem sap chemical composition as measured in the stems of two Norway Spruce (Picea abies [L.] Karst.) trees in the Fichtelgebirge region of Northern Bavaria, Germany. Dielectric constant and xylem sap flux were monitored continuously from June through October 1995, at several heights along the tree trunks. At the end of the measurement series, each tree was harvested, and its xylem sap extracted and analyzed to determine the concentrations of amino acids and cations. Results show that the sap flux density was correlated with vapor pressure deficit (VPD) at all heights in the stem. In contrast, the xylem tissue dielectric constant is influenced by VPD but can exhibit a significant temporal lag relative to changes in VPD. This lag varies with position along the tree trunk. The temporal variability of the dielectric constant is compared with both trees at several positions along the tree trunks. Results of xylem sap chemical analysis are presented. We show that spatial and temporal variability in the xylem tissue dielectric constant is influenced not only by water content, but by variations in xylem sap chemistry as well. This has important implications for microwave remote sensing of forested landscapes, as useful information may be acquired regarding stand physiology and water relations and where variations in dielectric properties within individual trees and across geographic areas can be significant error sources for forest inventory mapping.     

Where is the psychology going? Structural fault lines revealed by psychologists' use of Kuhn.

Psychologists' appropriation of language and ideas from Thomas Kuhn's (1962, 1970b) The Structure of Scientific Revolutions reveals deep and contradictory concerns about truth, science, and the progress of the field. The author argues that psychologists, uncomfortably straddling natural and social science traditions, reference Structure for 2 reasons largely overlooked: first, because it presents an intermediate, naturalistic position in the war between relativist and rationalist views of scientific truth, and second, because it presents a psychologized model of scientific change. The author suggests that the history of this mutual influence--psychologists being influenced by Kuhn and vice versa--may usefuly inform current practices of psychological science. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Progress in the development of polymer cholesteric liquid crystal flakes for display applications

Polymer cholesteric liquid crystal (PCLC) flake technology is being developed as an alternative display technology for flexible, reflective particle displays. The motion of PCLC flakes suspended in a host fluid can be controlled with an electric field, creating a way to electrically control the flakes' ability to brightly reflect light that is circularly polarized. The PCLC flake/host fluid dispersion has been successfully microencapsulated both in a polymer matrix and in gelatin micro-capsules. Microencapsulation will not only expand the applications scope of the technology, but also may aid in addressing some potential problem areas that are inherent to many forms of particle display technology. A second important development in PCLC flake technology involves the manufacture of shaped flakes based on soft lithography techniques. The size and shape of a flake impact its reorientation, and uniformly shaped flakes respond in a similar manner. The unique reflective properties of PCLC flakes also provide possible applications in areas such as optics and photonics, switchable ‘smart windows’ or conformal coatings, and information displays such as ‘electronic paper.’     

The OKT3 Antibody Response Study: a multicentre study of human anti-mouse antibody (HAMA) production following OKT3 use in solid organ transplantation

Human anti-murine antibody titres following patient exposure to the monoclonal antibody Orthoclone OKT3 (muromonab-CD3) are determined by laboratories using diverse analytical methods which are not standardized and whose concordance is not established. A multicentre study group therefore compared testing for IgG anti-OKT3 antibody among seven laboratories. A set of 270 sera was obtained from 30 heart, 30 kidney and 30 liver transplant recipients with no previous exposure to OKT3 who were receiving OKT3 for induction immunosuppression. Sera were collected from each patient prior to and at 24 +/- 2 days and 31 +/- 2 days following initial OKT3 exposure. Identical aliquots of all 270 sera were tested for IgG anti-OKT3 antibody by each laboratory. In addition, the limit of detection of each laboratory's method was estimated by titration of an affinity-purified IgG anti-OKT3 reference material of known concentration. Anti-OKT3 antibody formation differed greatly among the three organ groups. Cardiac patients demonstrated the least sensitization and almost exclusively lower titres, while kidney recipients had more frequent and higher titre antibody formation. Liver recipients yielded the highest sensitization rate and the most frequent high titre sera. Importantly, the seven laboratories differed widely in the number of pretreatment sera reported as positive (ranging from 0% to 41% among laboratories), the number of post-OKT3 sera reported as positive (17-63%), the number of post-OKT3 samples with titre > or = 1000 (2-31%), and the number of patients sensitized 19-69%). Concordance among laboratories was highly variable, with interlaboratory agreement ranging from 38% to 83% on the sample titres assigned to 180 post-OKT3 sera. Many of the discordant results were consistent with differences in the limit of detection of the analytical methods, which ranged from 0.19 microgram/ml to > or = 15 micrograms/ml, a nearly 100-fold difference among laboratories. This study demonstrated the presence of both good concordance and significant discordance among laboratories in determining human anti-mouse antibody titres, and demonstrated that common titre categories (100, 1000, 10,000) were not equivalent among laboratories. The level of concordance among methods should be considered when comparing anti-OKT3 antibody results from different centres and their correlation with clinical events. Universal comparative testing, patterned after proficiency testing programmes, is needed to assess differences among laboratories and to bring uniformity and a sound interpretative basis to this field of testing.     

Trajectories leading to school-age conduct problems.

The present study applied a semiparametric mixture model to a sample of 284 low-income boys to model developmental trajectories of overt conduct problems from ages 2 to 8. As in research on older children, 4 developmental trajectories were identified: a persistent problem trajectory, a high-level desister trajectory, a moderate-level desister trajectory, and a persistent low trajectory. Follow-up analyses indicated that initially high and low groups were differentiated in early childhood by high child fearlessness and elevated maternal depressive symptomatology. Persistent problem and high desister trajectories were differentiated by high child fearlessness and maternal rejecting parenting. The implications of the results for early intervention research are discussed, with an emphasis on the identification of at-risk parent-child dyads. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Cytokine responses of human lung cells (BEAS-2B) treated with micron-sized and nanoparticles of metal oxides compared to soil dusts

Background  

The induction of cytokines by airway cells in vitro has been widely used to assess the effects of ambient and occupational particles. This study measured cytotoxicity and the release of the proinflammatory cytokines IL-6 and IL-8 by human bronchial epithelial cells treated with manufactured nano- and micron-sized particles of Al₂O₃, CeO₂, Fe₂O₃, NiO, SiO₂, and TiO₂, with soil-derived particles from fugitive dust sources, and with the positive controls LPS, TNF-α, and VOSO₄.     

Depression in college students: Personality and experiential factors.

In this study we examined the nature of college-student depression and its relation to personality variables and to experiences unique to college life. Thirty-five depressed and 39 nondepressed students completed a depression inventory, a series of personality scales, and a survey developed to investigate the impact of potentially distressing components of college life. Results revealed that college-student depression, though mild in intensity, represents a serious problem: Three quarters of depressed students had been depressed for more than 3 months, and half had contemplated suicide. Both personality characteristics and experiential variables were found to be significantly related to depression, together accounting for approximately half the variance. We examined individual personality traits and experiential variables that were found to differ between depressed and nondepressed subjects, and we discuss the implications of the findings for counseling and for future research. (PsycINFO Database Record (c) 2010 APA, all rights reserved)