Monoacylglycerols derived from butter oil by Penicillium roquefortii in suspension cultures

Structural isomers of monoacylglycerols (monoglycerides, MAGs) were identified and compared after degradation of butter oil by two strains of Penicillium roquefortii and a commercial lipase from P roquefortii (EC 3.1.1.3) at pH 7.0 and 10 °C. The conditions were selected as they were comparable with those used in the manufacture of blue mould‐ripened cheese. The commercial lipase was selected to compare with the fungal strains in terms of acyl migration. Results showed that the main isomers formed by lipolysis with the commercial lipase were sn‐2 MAGs (64 mol%), whilst spores and emerging mycelia of P roquefortii produced mainly sn‐1(3) MAGs (83–90 mol%). The work reported here may lead to further assessment of different MAG structural isomers as natural preservatives in foods and dairy products. © 2002 Society of Chemical Industry     

Immunological responses from non-exposed donors to malaria antigens: implications for immunity and pathology

An approach to identification of epitopes suitable for vaccine development has been to locate regions of malaria target antigens that are recognized by individuals with clinical immunity. This has applied to identification of T- and B-cell epitopes. It is now realized, however, that T cells from individuals without prior exposure to malaria can respond to malaria parasites, malaria proteins, and peptides copying protein sequences. Such observations raise questions about which epitopes we should be targeting for vaccine development, but also challenge our understanding of immunological memory. Such responses from non-exposed individuals may also be important in expression of disease symptoms.     

Teachers' and deaf students' perceptions of communication ease and engagement.

Collected ratings of communication ease and of engagement for 95 deaf junior and senior high school students (aged 12–21 yrs) in a large, urban, multiethnic school for the deaf. Ratings were made by the students' English teachers and by the students themselves. High teacher ratings for communication ease and engagement were associated with high academic achievement as measured by the Stanford Achievement Test. High student ratings for communication ease were also associated with academic achievement. The 2 best predictors of academic achievement were student-rated communication ease and teacher-rated engagement. Findings suggest that it is important for teachers to be sensitive to the level of communication comfort of their students because students who enjoy easier communication may be more likely to be engaged, or more actively involved, in classroom learning and to demonstrate higher academic achievement. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Unsubstantiated claims of false memory and essential responsibilities.

Comments on the assertion of K. S. Pope (see record 83-37387) that the confirmation that supposedly validates claims about the false memory syndrome, its antecedents, and its rampant magnitude needs to be made available and carefully examined. The author considers why there has been acceptance of unsubstantiated claims of false memory, and also points to essential responsibilities for psychologists whose clients have abuse memories. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Explanation-based learning as justification of knowledge

Abstract: This paper describes the use of the explanation-based learning (EBL) machine learning technique in the practical domain of knowledge acquisition for expert systems. A knowledge acquisition tool, EBKAT (Explanation-Based Knowledge Acquisition Tool), is described, which may be used in the development of knowledge bases for diagnostic expert systems. The functioning of EBKAT attempts to combine the full potential of a domain expert's skills and the power of explanation-based machine learning techniques. The EBL component is not employed in the acquisition of the knowledge base rules but is used to justify the knowledge entered and to relate it to the knowledge already in the system. It is suggested that the EBKAT tool goes some way towards overcoming the knowledge acquisition bottleneck and results in the acquisition of knowledge which is rich in contextual information.     

Requirement of major histocompatibility complex-compatible microenvironment for spleen colony formation (CFU-S on day 12 but not on day 8)

The objectives of this study were 1) to evaluate interleukin-8 concentrations in cervical secretions in predicting preterm delivery, microbial invasion of the amniotic cavity and histologic chorioamnionitis in patients with preterm labor and intact membranes and 2) to compare the diagnostic value of interleukin-8 with fetal fibronectin determinations in predicting preterm delivery, microbial invasion of the amniotic cavity and histologic chorioamnionitis in patients with preterm labor and intact membranes. Interleukin-8 and fetal fibronectin were assayed in cervical secretions in 106 patients with singleton pregnancies and intact membranes admitted for preterm labor. Amniotic fluid obtained by amniocentesis was cultured and placentas (No = 43) analyzed for the presence of chorioamnionitis. The prevalence of pregnancies delivered preterm was 46.2% (49/106) and 15.09% (16/106) of amniotic fluid cultures were positive. Interleukin-8 levels in cervical secretions were significantly increased in patients who delivered preterm (p < or = 0.0001), in presence of positive amniotic fluid culture (p = 0.0016) and histological chorioamnionitis (p = 0.008) than in patients with negative findings. Receiver-operator characteristics curve analysis showed that an interleukin-8 value > 450 pg/ml is comparable to that of a fetal fibronectin value > 50 ng/ml in predicting preterm delivery (p = 0.247). Among patients who delivered preterm interleukin-8 concentrations > 860 pg/ml predicted a positive amniotic fluid culture with a sensitivity of 81.2% and a specificity 66.6%. Further, in patients who delivered preterm and had a negative amniotic fluid culture, IL-8 concentrations > 480 pg/ml predicted histological chorioamnionitis with a sensitivity 78.5% and specificity 61.5%. A positive fetal fibronectin > 50 ng/ml was not predictve of either a positive amniotic fluid culture or the presence of histological chorioamnionitis. In conclusion, increased concentrations of interleukin-8 and fetal fibronectin are associated with impending delivery and their diagnostic value seems comparable. However, interleukin-8 concentrations identify patients at risk of a positive amniotic fluid culture and the presence of histological chorioamnionitis. Measurement of interleukin-8 in cervical secretion is a non-invasive method to identify patients at risk for both preterm delivery and intrauterine infection.