Cell-mediated cytotoxicity: a predictor of chronic rejection in pediatric HLA haploidentical renal transplants

BACKGROUND: Recipient antidonor cytotoxic T-cell activity has been associated with graft loss and acute rejection in renal allograft recipients. The role of immunologic mechanisms in the development of chronic graft rejection is controversial. We analyzed all living related renal transplants performed at Children's Hospital (Boston, MA) from 1983 to 1995 to assess whether cell-mediated cytotoxicity, determined in vitro and measured before transplantation, was predictive of chronic rejection. METHODS: Eighty-three patients were studied retrospectively. Fifty-seven patients with one haplotype-matched renal transplants from living related donors were studied to determine the association between cell-mediated lympholysis (CML) level, acute rejection, chronic rejection, and graft failure. Acute rejection was defined by the decision to treat. Chronic rejection was defined by histology and/or the absolute serum creatinine value using an increasing serum creatinine level >1.0 mg/dl for children less than 3, a creatinine level >1.5 mg/dl for children between 3 and 10 years of age, and a creatinine level >2.0 mg/dl for children above 10 years of age. Return to dialysis or retransplantation was considered graft failure. RESULTS: Of the 57 haploidentical patients, there were 33 males and 24 females. The mean age at transplant was 11.1 years (SD=6.7). Twelve patients developed chronic rejection, 24 patients developed acute rejection, and 7 patients had graft failure. Pretransplant cytotoxic T lymphocyte activity was associated with chronic rejection (P=0.001) and graft failure (P=0.013) but only marginally with acute rejection (P=0.058). Controlling for age and sex, Cox's proportional hazards model revealed that CML level was predictive of time to chronic rejection (P<0.01) but not acute rejection (P=0.11). It was estimated that every 1-unit increase in CML level raises the monthly risk of chronic rejection by 7%. Ten children received HLA-identical kidneys from their siblings. There were no episodes of chronic rejection after 5 years. Two patients with high CML levels had episodes of acute rejection; both patients responded to treatment. CONCLUSION: Our data demonstrate an association between pretransplant cell-mediated cytotoxicity and the occurrence of chronic rejection in living related one-haploidentical renal transplants in pediatric patients.     

Self-tuned programmable active-R square wave generator

Self-tuning of a digitally programmable active-R square wave generator is investigated. A single-chip-mode microprocessor is used as a microcontroller. The microcontroller continuously monitors and automatically corrects the shift in the output frequency that may be caused by temperature drift, component mismatch, aging, and/or any other environmental changes     

Qualitative response of safety injection system for a large size break in the reactor coolant system cold leg

Safety injection system, accumulator injection system and residual heat removal system of CHASNUPP-1 were simulated using the computer code APROS. We observed the qualitative response of the simulated system during injection and re-circulation phases after LOCA. During rapid depressurization of SRC system due to leakage, these systems started coolant injection in the SRC system as per plant requirement. Different thermal-hydraulic parameters of the respective systems are presented and discussed. Results obtained are in good agreement with the reported document of the reference power plant.     

Finite element modeling of transient heat transfer and microstructural evolution in welds: Part II. Modeling of grain growth in austenitic stainless steels

During welding, structures are subjected to localized heating and cooling cycles, as described in Part I.[1] A mathematical model is proposed to determine the metallurgical changes that occur in austenitic stainless steel due to the welding thermal cycle. The proposed kinetic model computes the austenite grain growth as a function of time and temperature. It is based on a Zener pinning grain growth model. The results obtained indicate that the model is in good agreement with the experimental data reported in the literature. Furthermore, it was observed that rewriting the kinetic constant in the grain growth equation as a function of the peak temperature led to improved results for the majority of the cases examined.     

Calendering analysis without the lubrication approximation

A general-purpose finite-element program has been used to simulate the flow of Newtonian, power-law, and viscoelastic fluids in calendering. The analysis is fully two-dimensional and does not make use of the lubrication approximation. Isothermal and nonisothermal calendering is studied and the results are compared with predictions from the lubrication approximation. The free surface is determined and circulatory flow patterns are predicted in the melt bank. Detailed calculations have been performed for a rigid poly(vinyl chloride) (PVC) resin that exhibits slip at the wall using rheological data for the melt and machine parameters. The results include determination of the shape and location of the free surface, vortex patterns, temperature and pressure distributions, and predictions of roll-separating force, torque, and power consumption. Comparisons are made with experimental data available in the literature.     

Energy-efficient clustering in mobile ad-hoc networks using multi-objective particle swarm optimization

A mobile ad hoc network (MANET) is dynamic in nature and is composed of wirelessly connected nodes that perform hop-by-hop routing without the help of any fixed infrastructure. One of the important requirements of a MANET is the efficiency of energy, which increases the lifetime of the network. Several techniques have been proposed by researchers to achieve this goal and one of them is clustering in MANETs that can help in providing an energy-efficient solution. Clustering involves the selection of cluster-heads (CHs) for each cluster and fewer CHs result in greater energy efficiency as these nodes drain more power than noncluster-heads. In the literature, several techniques are available for clustering by using optimization and evolutionary techniques that provide a single solution at a time. In this paper, we propose a multi-objective solution by using multi-objective particle swarm optimization (MOPSO) algorithm to optimize the number of clusters in an ad hoc network as well as energy dissipation in nodes in order to provide an energy-efficient solution and reduce the network traffic. In the proposed solution, inter-cluster and intra-cluster traffic is managed by the cluster-heads. The proposed algorithm takes into consideration the degree of nodes, transmission power, and battery power consumption of the mobile nodes. The main advantage of this method is that it provides a set of solutions at a time. These solutions are achieved through optimal Pareto front. We compare the results of the proposed approach with two other well-known clustering techniques; WCA and CLPSO-based clustering by using different performance metrics. We perform extensive simulations to show that the proposed approach is an effective approach for clustering in mobile ad hoc networks environment and performs better than the other two approaches.