Growth and function of human parathyroid tissue transplanted to athymic mice

The morphology, cell proliferation and function of transplanted normal, hyperplastic and adenomatous human parathyroid tissue was studied after transplantation to athymic mice. The iPTH was evaluated in relation to morphology. Human parathyroid tissue collected during surgery for hyperparathyroidism was implanted subcutaneously into athymic mice (nu/nu-BALB/cA) and was analysed 1, 4, 7 and 12 weeks after transplantation. The transplants were examined by light and electron microscopy and by autoradiography after continuous infusion of 3H-thymidine. The relative amount of viable tissue was evaluated using a computer image analysing programme. Graft function was evaluated by measuring human iPTH in mouse serum. A transplant take ratio of 93% was observed. The proliferation rate in adenoma grafts at 12 weeks after transplantation was five and fifteen times that observed in normal and hyperplastic transplants, respectively. In normal and adenoma groups, a continuous increase in iPTH concentrations was observed, but in the hyperplastic group the iPTH remained on the same level. The secretion of iPTH in relation to the amount of transplanted tissue and the fraction of viable tissue was at the same level at 12 weeks in normal and adenomatous grafted animals. In conclusion, human parathyroid tissue was successfully transplanted and maintained its original structure. The growth potential, but not the iPTH secretion, was significantly higher in adenoma grafts compared to grafts from hyperplastic and normal glands.     

An Extended Replication of an Experiment for Assessing Methods for Software Requirements Inspections

We have performed an extended replication of the Porter-Votta-Basili experiment comparing the Scenario method and the Checklist method for inspecting requirements specifications using identical instruments. The experiment has been conducted in our educational context represented by a more general definition of a defect compared to the original defect list. Our study involving 24 undergraduate students manipulated three independent variables: detection method, requirements specification, and the order of the inspections. The dependent variable measured is the defect detection rate. We found the requirements specification inspected and not the detection method to be the most probable explanation for the variance in defect detection rate. This suggests that it is important to gather knowledge of how a requirements specification can convey an understandable view of the product and to adapt inspection methods accordingly. Contrary to the original experiment, we can not significantly support the superiority of the Scenario method. This is in accordance with a replication conducted by Fusaro, Lanubile and Visaggio, and might be explained by the lack of individual defect detection skill of our less experienced subjects.     

Clinical trial results support a preference for using CLA preparations enriched with two isomers rather than four isomers in human studies

CLA mixtures are now commercially available. They differ from each other with respect to their content of CLA isomers and their degree of purification. As a group of natural FA, CLA have been widely assumed to be safe. However, the suspected presence of both impurities and particular isomers might induce undesirable side effects. Despite this potential health risk, only a few CLA preparations have been tested under rigorous conditions for clinical efficacy and safety. Based on the limited results available, it is possible to suggest that preparations enriched in c9,t11 and t10,c12 isomers are preferable for human consumption compared to preparations containing four isomers, in terms both of safety and efficacy.     

Fast kriging of large data sets with Gaussian Markov random fields

Spatial data sets are analysed in many scientific disciplines. Kriging, i.e. minimum mean squared error linear prediction, is probably the most widely used method of spatial prediction. Computation time and memory requirement can be an obstacle for kriging for data sets with many observations. Calculations are accelerated and memory requirements decreased by using a Gaussian Markov random field on a lattice as an approximation of a Gaussian field. The algorithms are well suited also for nonlattice data when exploiting a bilinear interpolation at nonlattice locations.     

Strategies for increasing evaporative cooling during simulated desert patrol mission

The study evaluated the efficiency of two heat dissipation strategies under simulated desert patrol missions. Ten men participated in four trials, during which they walked on a treadmill (45°C, 20% relative humidity), carrying a load of 35 kg; two 50-min walks were separated by a 20-min rest. Cooling strategies, provided by an ambient air-ventilated vest (active cooling condition, AC), or water spraying of the skin during the rest (passive cooling condition, PC), in addition to reduced clothing and open zippers, were compared to conditions with full protective (FP) clothing and naked condition (NC). Skin temperature was higher during NC (37.9 ± 0.4°C; p < 0.001), and rectal temperature and heart rate were higher during FP (38.6 ± 0.4°C, p < 0.001 and 145 ± 12, p < 0.001, respectively), compared to other conditions. Four subjects terminated the trial prematurely due to signs of heat exhaustion in FP. Both cooling strategies substantially improved evaporative cooling.     

Proteomic studies in animal models of diabetes

The aim of this review is to provide an overview of proteomic studies in animal models of diabetes and to give some insight into the different methods available today in the rapidly developing field of proteomics. A summary of 31 papers published between 1997 and 2007 is presented. For instance, proteomics has been used to study the development of both type 1 and type 2 diabetes, diabetic complications in tissues like heart, kidney and retina and changes after treatment with anti-diabetic drugs like peroxisome proliferator-activated receptors agonists. Together, these studies give a good overview of a number of experimental approaches. Proteomics holds the promise of providing major contributions to the field of diabetes research. However, to achieve this, a number of issues need to be resolved. Appropriate data representation to facilitate data comparison, exchange, and verification is required, as well as improved statistical assessment of proteomic experiments. In addition, it is important to follow up the results with functional studies to be able to make biologically relevant conclusions. The potential of proteomics to dissect complex human disorders is now beginning to be realized. In the future, this will result in new important information concerning diabetes.     

The effect of flooring on musculoskeletal symptoms in the lower extremities and low back among female nursing assistants

This study examines the effect of changing the floor from a 2-mm homogenous vinyl floor to a 4-mm heterogeneous vinyl floor (1.5-mm wear layer and 2.5-mm foam layer) on musculoskeletal symptoms in the lower extremities and low back among nursing assistants in a geriatric care centre. A pre-post design with a reference group consisting of nursing assistants from a similar geriatric care centre was used. Follow-up measurements were carried out 12 and 24 months after the intervention by means of questionnaires. At the 1-year follow-up, the pain intensity score in the feet of the intervention group had decreased compared with the baseline value and remained statistically significant at the 2-year follow-up. The decrease in pain intensity score of the feet in the intervention group was statistically significantly different from the reference group, both after 1 and 2 years. PRACTITIONER SUMMARY: The results show the importance of flooring in the workplace with regard to reducing musculoskeletal symptoms. Appropriate flooring is especially important in the female-dominated health care sector, where workers must stand or walk for long periods.