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M Pfau KE Kendall T Reichert SA Hellweg W Lee KJ Tusing TO Prosise 《The Journal of communication》1997,47(4):6-26
This study examines the influence of five communication modalities on voters' perceptions of candidates during what Popkin terms 'the distant phase' of the presidential primary campaign. During the final week of the New Hampshire primary campaign, researchers surveyed 315 prospective votes in four states whose primaries followed New Hampshire's by 1, 2, 4, and 5 weeks. The results revealed that political talk radio exerted the greatest influence on voters' perceptions of Bob Dole and considerable impact on perceptions of Steve Forbes. In addition, prospective voters' conversations with other people, television news, and candidate spots (only for Forbes) exerted modest influence on voters' perceptions of Republican candidates. 相似文献
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SS Taremi B Beyer M Maher N Yao W Prosise PC Weber BA Malcolm 《Canadian Metallurgical Quarterly》1998,7(10):2143-2149
Efficient proteolytic processing of essential junctions of the hepatitis C virus (HCV) polyprotein requires a heterodimeric complex of the NS3 bifunctional protease/helicase and the NS4A accessory protein. A single-chain recombinant form of the protease has been constructed in which NS4A residues 21-32 (GSVVIVGRIILS) were fused in frame to the amino terminus of the NS3 protease domain (residues 3-181) through a tetrapeptide linker. The single-chain recombinant protease has been overexpressed as a soluble protein in E. coli and purified to homogeneity by a combination of metal chelate and size-exclusion chromatography. The single-chain recombinant protease domain shows full proteolytic activity cleaving the NS5A-5B synthetic peptide substrate, DTEDVVCCSMSYTWTGK with a Km and k(cat) of 20.0 +/- 2.0 microM and 9.6 +/- 2.0 min(-1), respectively; parameters identical to those of the authentic NS3(1-631)/NS4A(1-54) protein complex generated in eukaryotic cells (Sali DL et al., 1998, Biochemistry 37:3392-3401). 相似文献
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