Acyl exchange between oleoyl-CoA and phosphatidylcholine in microsomes of developing soya bean cotyledons and its role in fatty acid desaturation

Microsomes of developing soya bean cotyledons transfer oleate from oleoyl-CoA to phosphatidylcholine (PC) by two different mechanisms: one in which oleate transfer is accompanied by the release of free CoA and another which results in the exchange of oleate from oleoyl-CoA for unsaturated 18-carbon fatty acids of PC. The acyl exchange can be demonstrated only when bovine serum albumin is present in the incubation medium. ATP-dependent acyl-CoA synthetase is not involved in the exchange process, which apparently does not require any cofactors. In light of this exchange process, the oleate desaturase system was reinvestigated in order to determine what the actual substrate for this system is. Upon incubation of microsomes with high concentrations of [¹⁴C] oleoyl-CoA, bovine serum albumin and NADH, it could be conclusively demonstrated that most oleic acid is desaturated while part of the PC molecule. The amounts of [¹⁴C] linoleoyl-CoA formed could be explained entirely by the acyl exchange. The physiological significance of the acyl exchange system is discussed. A new method for separation of acyl-CoA from other lipids and free CoA using reversed phase column chromatography also is described.     

Difference algebra and system identification

The framework of differential algebra, especially Ritt’s algorithm, has turned out to be a useful tool when analyzing the identifiability of certain nonlinear continuous-time model structures. This framework provides conceptually interesting means to analyze complex nonlinear model structures via the much simpler linear regression models. One difficulty when working with continuous-time signals is dealing with white noise in nonlinear systems. In this paper, difference algebraic techniques, which mimic the differential-algebraic techniques, are presented. Besides making it possible to analyze discrete-time model structures, this opens up the possibility of dealing with noise. Unfortunately, the corresponding discrete-time identifiability results are not as conclusive as in continuous time. In addition, an alternative elimination scheme to Ritt’s algorithm will be formalized and the resulting algorithm is analyzed when applied to a special form of the nfir model structure.     

Highly Porous Geopolymers Through Templating and Surface Interactions

The natural porosity of geopolymers and the simplicity of geopolymer synthesis make them a potential candidate for the formation of highly porous ceramics. Here, the synthesis of highly porous (≈70 vol% or more) metakaolin geopolymer is demonstrated using a novel emulsion preparation with one‐pot curing and hydrophobization of the interior pores with alkylalkoxysilanes. Using mercury intrusion porosimetry, tailoring of the characteristic percolation pore size is demonstrated over a range of ≈200 nm to 10 μm. Using powder X‐ray diffraction, reactivity was shown to be decreased versus typical geopolymers, but substantial geopolymerization still occurred and the samples formed cohesive monoliths. Optional calcination under inert conditions allowed for formation of a glassy ceramic with a notable SiC phase, as well as further increasing the porosity by removing the hydrophobic pore coatings.     

Single-step formation of a biorecognition layer for assaying histidine-tagged proteins

The purpose of this work was to develop a simple procedure for the creation of a specific biorecognition layer for histidine-tagged (His-tagged) molecules. Such a layer was prepared by the spontaneous fusion of vesicles containing readily available plain (DOPC) and iminodiacetic acid (DOGS-NTA) phospholipids on a silica surface resulting in the formation of an NTA-containing supported lipid bilayer. The frequency surface acoustic waveguide device which supports Love waves was used to follow the real-time formation of the biorecognition layer. The mole percent of the DOGS-NTA phospholipids in the supported bilayer was optimized by following the kinetics of the fusion for the different NTA-containing lipids. Fluorescently labeled lipids were used with observations of the fluorescence recovery after photobleaching to confirm the presence of lipid bilayers. After saturating all NTA-molecules with Ni(2+), the binding of a His-tagged protein fragment within the concentration range of 0.04 and 0.4 mM to a 5 mol % DOGS-NTA/DOPC was detected; binding curves were used to calculate the apparent association constant k(on) = 2.56 x 10(4) M(-)(1) s(-)(1), dissociation constant k(off) = 1.3 x 10(-)(3) s(-)(1), and equilibrium constant k(eq) = 1.97 x 10(7) M(-)(1). The described method could find significant applications as a generic technique for preparing biorecognition layers for His-tagged proteins. In addition, the acoustic waveguide device, which provides high sensitivity together with flexibility in terms of the substrate material used, is shown to be an attractive alternative to direct optical biosensors.     

How accurately does the public perceive differences in transport risks?: An exploratory analysis of scales representing perceived risk

This paper probes the extent to which the public accurately perceives differences in transport risks. The paper is based on a survey of a random sample of the Norwegian population, conducted in September 2003. In the survey, respondents were asked: “How safe do you think it is to travel by means of (bus, train, etc.)?” Answers were given as: very safe, safe, a little unsafe, and very unsafe. A cursory examination of the answers suggested that the Norwegian public was quite well informed about differences in the risk of accident between different modes of transport, as well as between groups formed according to age and gender for each mode of transport. This paper probes the relationship between statistical estimates of risk and summary representations of perceived risk more systematically. It is found that the differences in fatality rate between different modes of transport are quite well perceived by the Norwegian public, irrespective of the way in which perceived risk is represented numerically. The relationship between statistical estimates of risk and numerical representations of perceived risk for each mode of transport is more sensitive to the choice of a numerical representation of perceived risk. A scale in which the answer “very safe” is assigned the value of 0.01 and the answer “very unsafe” is assigned the value of 10 is found to perform quite well. When the perception of risk is represented numerically according to this scale, a positive correlation between statistically estimated risk and perceived risk is found in seven of the eight comparisons that were made to determine how well variation in accident rates according to age and gender for car occupants, car drivers, cyclists and pedestrians are perceived.     

Hazard perception and driving experience among novice drivers

The crash risk of novice drivers decreases rapidly during the first few months of post-licensing driving, indicating that some important safety-relevant traffic skills are learnt during this period. The present study tested the hypothesis that the risk decrease is related to improved hazard perception skills. A video-based hazard perception/reaction test, measuring reaction times to 31 traffic scenes, was administered to three groups of drivers, having held a licence for 1, 5, and 9 months, respectively, and to a group of drivers who had held their licences for several years. Average reaction times tended to decrease with experience, but the decrease was not significant. There were some significant differences in the expected direction for individual test items, indicating a possible effect of experience. One half of the situations were presented together with a secondary task, in order to investigate effects of increased mental load on hazard perception. Male novice drivers had relatively longer reaction times with the secondary task, compared to both female novice drivers and male experienced drivers. It is concluded that hazard perception as tested here is probably only a minor factor in explaining the initial risk decrease among novice drivers.     

Output dead-beat control for nonlinear systems with one zero at infinity

The problem of output dead-beat control, i.e. one-step zeroing of the output, is considered for nonlinear systems with discrete-time controllers. The nonlinear system is assumed to have one zero at infinity. Conditions for the existence of the dead-beat control for small sampling intervals are derived. The closed-loop system is shown to be stable in the sense that all states converge to the origin if the controlled system is minimum phase.     

Traffic safety among motorcyclists in Norway: A study of subgroups and risk factors

Although it is widely recognized that motorcyclists have a particularly high accident risk, our knowledge of the mechanisms producing this accident risk is incomplete. The aims of the present paper are to identify subgroups of motorcyclists with a particularly high accident risk and to identify the relevant risk factors at work. The study presented in this paper relies both on a questionnaire (N = 3356) relating rider characteristics, behaviors and accident risk, and analyses of fatal motorcycle accidents (ca. 100) from 2005 to 2008 in Norway. The results reveal that riders of racing replica bikes (sport bikes), and riders younger than 19 years, including especially youths (16–17 years) riding light motorcycles (≤125 cm³), are subgroups of Norwegian motorcyclists with particularly high accident risks. Analysis of fatal motorcycle accidents reveals that about half of the fatal accidents involve sport bikes. Nearly all fatal accidents with sport bikes involved excessive speed. The combination of low age, low experience, risky behavior and “unsafe” attitudes seems to be a particular potent risk factor for Norwegian motorcyclists.