Preparation of Human Milk Fat Substitutes from Lard by Lipase‐Catalyzed Interesterification Based on Triacylglycerol profiles

Human milk fat substitutes (HMFSs) with triacylglycerol profiles highly similar to those of human milk fat (HMF) were prepared from lard by physical blending followed by enzymatic interesterification. Based on the fatty acid profiles of HMF, different vegetable and single‐cell oils were selected and added to the lard. Blend ratios were calculated based on established physical blending models. The blended oils were then enzymatically interesterified using a 1,3‐regiospecific lipase, Lipozyme RM IM (RML from Rhizomucor miehei immobilized on Duolite ES562; Novozymes A/S, Bagsværd, Denmark), to approximate HMF triacylglycerol (TAG) profiles, particularly with respect to the distribution of palmitic acid in the sn?2 position. The optimized blending ratios were determined to be: lard:sunflower oil:canola oil:palm kernel oil:palm oil:algal oil:microbial oil = 1.00:0.10:0.50:0.13:0.12:0.02:0.02. The optimized reaction conditions were determined to be: enzyme load of 11 wt%, temperature of 60 °C, water content of 3.5 wt%, and reaction time of 3 hours. The resulting product was evaluated for total and sn?2 fatty acids, polyunsaturated fatty acids, and TAG composition. A high degree of similarity was obtained, indicating the great potential of the product as a fat alternative for use in infant formulas.     

Colonization and Infection of the Skin by S. aureus: Immune System Evasion and the Response to Cationic Antimicrobial Peptides

Staphylococcus aureus (S. aureus) is a widespread cutaneous pathogen responsible for the great majority of bacterial skin infections in humans. The incidence of skin infections by S. aureus reflects in part the competition between host cutaneous immune defenses and S. aureus virulence factors. As part of the innate immune system in the skin, cationic antimicrobial peptides (CAMPs) such as the β-defensins and cathelicidin contribute to host cutaneous defense, which prevents harmful microorganisms, like S. aureus, from crossing epithelial barriers. Conversely, S. aureus utilizes evasive mechanisms against host defenses to promote its colonization and infection of the skin. In this review, we focus on host-pathogen interactions during colonization and infection of the skin by S. aureus and methicillin-resistant Staphylococcus aureus (MRSA). We will discuss the peptides (defensins, cathelicidins, RNase7, dermcidin) and other mediators (toll-like receptor, IL-1 and IL-17) that comprise the host defense against S. aureus skin infection, as well as the various mechanisms by which S. aureus evades host defenses. It is anticipated that greater understanding of these mechanisms will enable development of more sustainable antimicrobial compounds and new therapeutic approaches to the treatment of S. aureus skin infection and colonization.     

Indoor air quality and energy performance of air-conditioned office buildings in Singapore

An integrated indoor air quality (IAQ)-energy audit methodology has been developed in this study in Singapore, which provides a rigorous and systematic method of obtaining the status-quo assessment of an 'IAQ signature' in a building. The methodology entails a multi-disciplinary model in obtaining measured data pertaining to different dimensions within the built environment such as the physical, chemical, biological, ventilation, and occupant response characteristics. This paper describes the audit methodology and presents the findings from five air-conditioned office buildings in Singapore. The research has also led to the development of an indoor pollutant standard index (IPSI), which is discussed in this paper. Other performance indicators such as, the ventilation index and the energy index as well as the building symptom index (BSI) are also presented and discussed in the context of an integrated approach to IAQ and energy. Several correlation attempts were made on the various symptoms, indoor air acceptability, thermal comfort, BSI and IPSI, and while BSI values are found to correlate among them as well as with IAQ and THERMAL COMFORT acceptability, no such correlation was observed between BSI and IPSI. This would suggest that the occupants' perception of symptoms experienced as well as environmental acceptability is quite distinct from IAQ acceptability determined from empirical measurements of indoor pollutants, which reinforces the complex nature of IAQ issues.     

生命科学建筑及其发展趋势

用作生命科学研究的建筑物需要有专门的建设和服务元素的介入．才能提供和创造符合工艺流程状况的设施和环境。其中的专业实验室。布置时应针对用户进行环境定制和专业设施配置，并为对生物生命各个方面的高端观察提供便利。在分析生命科学建筑的特征厦发展趋势的同时，对其内部空间的使用进行了调查，为生命科学建筑设计提供佐证。     

Local thermal sensation and comfort study in a field environment chamber served by displacement ventilation system in the tropics

This paper presents a study of local thermal sensation (LTS) and comfort in a field environmental chamber (FEC) served by displacement ventilation (DV) system. The FEC, 11.12 m (L)×7.53 m (W)×2.60 m (H), simulates a typical office layout. A total of 60 tropically acclimatized subjects, 30 male and 30 female, were engaged in sedentary office work for 3 h. Subjects were exposed to three vertical air temperature gradients, nominally 1, 3 and 5 K/m, between 0.1 and 1.1 m heights and three room air temperatures of 20, 23 and 26 °C at 0.6 m height. The objective of this study is to investigate the mutual effect of local and overall thermal sensation (OTS) and comfort in DV environment. The results show that in a space served by DV system, at OTS close to neutral, local thermal discomfort decreased with the increase of room air temperature. The OTS of occupants was mainly affected by LTS at the arm, calf, foot, back and hand. Local thermal discomfort was affected by both LTS and OTS. At overall cold thermal sensation, all body segments prefer slightly warm sensation. At overall slightly warm thermal sensation, all body segments prefer slightly cool sensation.     

Rapid Quantification of Live Cell Receptors Using Bioluminescence in a Flow‐Based Microfluidic Device

The number of receptors expressed by cells plays an important role in controlling cell signaling events, thus determining its behaviour, state and fate. Current methods of quantifying receptors on cells are either laborious or do not maintain the cells in their native form. Here, a method integrating highly sensitive bioluminescence, high precision microfluidics and small footprint of lensfree optics is developed to quantify cell surface receptors. This method is safe to use, less laborious, and faster than the conventional radiolabelling and near field scanning methods. It is also more sensitive than fluorescence based assays and is ideal for high throughput screening. In quantifying β₁ adrenergic receptors expressed on the surface of H9c2 cardiomyocytes, this method yields receptor numbers from 3.12 × 10⁵ to 9.36 × 10⁵ receptors/cell which are comparable with current methods. This can serve as a very good platform for rapid quantification of receptor numbers in ligand/drug binding and receptor characterization studies, which is an important part of pharmaceutical and biological research.     

Sensors and Machine Learning Models to Prevent Cooktop Ignition and Ignore Normal Cooking

Fire Technology - Cooking equipment is involved in nearly half of home fires in the United States, with cooktop fires the leading cause of deaths and injuries in cooking-related fires. In this...     

Investigation of functionally graded metal foam thermal management system for solar cell

Concentrated photovoltaic cell (CPV) is a solar energy harvesting device that converts solar energy into electrical energy. However, the performance and efficiency of the CPV are heavily dependent on the temperature. Besides, nonuniformity of temperature distribution on the CPV will lead to thermal aging and affects the cycle life. Hence, an effective cooling system is required to remove excess heat generated to ensure that the CPV operates at optimum operating temperature with minimum variation of temperature. Metal foam is a new class of material that possesses huge potential for thermal management. In this study, a functionally graded metal foam is proposed for the CPV thermal management system. Computational thermal fluid dynamic analysis is conducted to investigate the effect of porosity and pore density on the flow field and thermal performance of the aluminum foam heat sink. The investigation results revealed that 10 PPI functionally graded aluminum foam heat sink with two stages of porosity gradient 0.794 and 0.682 produced the lowest pressure drop and highest thermal performance. Temperature difference of 3.9°C was achieved for a solar cell with total heat generation of 900 W under water mass flow rate of 20 gs⁻¹.