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排序方式: 共有925条查询结果,搜索用时 18 毫秒
1.
Dr. Shingo Naraki Mai Kakihara Sayuri Kato Dr. Yusuke Saga Dr. Kazuto Mannen Dr. Shohei Takase Dr. Akihito Takano Sayaka Shinpo Dr. Tsutomu Hosouchi Dr. Takahisa Nakane Dr. Hideyuki Suzuki Dr. Tetsuo Kushiro 《Chembiochem : a European journal of chemical biology》2021,22(11):1992-2001
Imperata cylindrica is known to produce a pair of triterpenes, isoarborinol and fernenol, that exhibit identical planar structures but possess opposite stereochemistry at six of the nine chiral centers. These differences arise from a boat or a chair cyclization of the B-ring of the substrate. Herein, we report the characterization of three OSC genes from I. cylindrica. IcOSC1 and IcOSC5 were identified as isoarborinol and fernenol synthases, respectively, while IcOSC3 was characterized as a multifunctional enzyme that produces glutinol and friedelin as its major products. Mutational studies of isoarborinol and fernenol synthases revealed that the residues surrounding the DCTAE motif partially affected the conformation of the B-ring during cyclization. Additionally, the IcOSC1-W255H mutant produced the rare triterpene boehmerol. The introduced histidine residue presumably abstracted a proton from the intermediary carbocation at C18 during the 1,2-rearrangement. Expression analysis indicated that all OSC genes were highly expressed in stems. 相似文献
2.
Tsutomu Tachibana Yuki Nishikawa Nakao Kubo Seiji Takeda 《International journal of molecular sciences》2021,22(1)
Floral organs have evolved from leaves for reproduction, and the morphological analyses help to understand the plant diversity and evolution. Habenaria radiata (syn. Pecteilis radiata) is a terrestrial orchid living in wetlands in Japan, Russia, South Korea, and China. The habitats of this plant in Japan have been reduced because of environmental destruction and overexploitation, and thus it is on the Red List of Japan as a Near Threatened species. One of the three petals of the H. radiata flower is called a lip or labellum, which resembles a flying white bird, egret, or white heron, with its proposed function being to attract pollinators. To understand the diversity of H. radiata plants in different areas, we examined the lip morphology and phylogeny of populations from eight habitats in the Kinki area, Japan. The complex shapes of the lips were quantified and presented as a radar chart, enabling characterization of the morphological difference among populations. Phylogenetic analysis with microsatellite markers that we generated showed the variation of genetic diversity among populations, suggesting the different degrees of inbreeding, outbreeding, and vegetative propagation. Our approach offers a basic method to characterize the morphological and genetic diversity in natural populations. 相似文献
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A generation method of artificial fluctuation data of wind power output considering smoothing effect
To use realistic wind power fluctuation data is important in simulating frequency change of power systems. It is well known that the smoothing effect must be taken into account when generating the artificial wind power output. Therefore, this paper proposed a new method to generate fluctuation waveform considering smoothing effect based on random number to fulfill the correlation among multiple sites and autoregression model. The effectiveness of the proposed method was tested by using practical wind speed data measured in Tohoku district. 相似文献
6.
Xuan Viet Cao Hanafi Ismail Azura A. Rashid Tsutomu Takeichi Thao Vo‐Huu 《乙烯基与添加剂工艺杂志》2014,20(4):218-224
Biocomposites were prepared from a kenaf core powder and recycled high‐density polyethylene/(natural rubber) blend by using an internal mixer at 165oC and 50 rpm. The effect of the filler content and the filler surface treatment was studied. Chemical modification of kenaf filler was performed with alkali pretreatment followed by treatment with silane. Scanning electron microscopy and infrared spectroscopy studies confirmed changes in the chemical compositions and structural characteristics induced through the modification. It was found that treated biocomposites offered higher tensile strength and tensile modulus, but lower elongation at break compared with untreated biocomposites. Lower water absorption and higher thermal stability of the resultant biocomposites were also obtained when treated fillers were used. J. VINYL ADDIT. TECHNOL., 20:218–224, 2014. © 2014 Society of Plastics Engineers 相似文献
7.
Tsutomu Iwashita Yoshiaki Kurobane Koji Azuma Yuji Makino 《Engineering Structures》2003,25(14):1815-1826
A series of tests, which reproduced brittle fracture starting from the region around the terminations of complete joint penetration groove welded joints, was conducted. The joint models under study represented a portion of welded beam flange-to-through diaphragm joints in beam-to-column connections. Various artificial weld defects were installed in the welded joints prior to testing. Test results were evaluated based on the failure assessment diagram approach specified in BS 7910. The conventional failure assessment diagrams predicted occurrences of brittle fracture erring on the very conservative side as expected, especially when applied to surface defects. The failure assessment diagram approach was modified by considering the effect of enhanced apparent toughness of material due to the loss of crack tip constraint. The modified failure assessment approach was found to predict the test results well. 相似文献
8.
Dr. Takuya Kobayakawa Kento Ebihara Yuzuna Honda Dr. Masayuki Fujino Dr. Wataru Nomura Prof. Naoki Yamamoto Dr. Tsutomu Murakami Prof. Hirokazu Tamamura 《Chembiochem : a European journal of chemical biology》2019,20(16):2101-2108
C34, a 34-mer fragment peptide, is contained in the HIV-1 envelope protein gp41. A dimeric derivative of C34 linked through a disulfide bridge at its C terminus was synthesized and found to display potent anti-HIV activity, comparable with that of a previously reported PEGylated dimer of C34REG. The reduction in the size of the linker moiety for dimerization was thus successful, and this result might shed some light on the mechanism of the suppression of six-helix bundle formation by these C34 dimeric derivatives. Addition of a Gly-Cys(CH2CONH2)-Gly-Gly motif at the N-terminal position of a C34 monomeric derivative significantly increased the anti-HIV-1 activity. This moiety functions as a new pharmacophore, and this might provide a useful insight into the design of potent HIV-1 fusion inhibitors. 相似文献
9.
Isotactic polypropylene filled with various contents of multi‐walled carbon nanotubes (MWCNTs) were fabricated by the injection molding technique and then rolled at room temperature. The unrolled samples (URS) and rolled samples (RS) were characterized by X‐ray diffraction studies, scanning electron microscopy, mechanical and micromechanical tests and differential thermal analyses. Although the URS exhibit the lamellar α‐crystal with a*‐axis orientation, the RS show the same crystals with both a*‐ and c‐axis orientation, which is explained by interlamellar and intralamellar slips and lamellar destruction. Scanning electron micrographs display distinct surface morphological features for both URS and RS. While the tensile strength of RS is higher than that of URS, the Young's modulus (Y) is found to be lower than that of URS. Anisotropy in microharness (H) parallel and perpendicular to the rolled direction has been detected, although H for both samples increases with increasing MWCNT contents. The average relationship H/Y ≈ 0.18 as estimated for URS is closer to the predicted value of 0.10 for polymers than the H/Y ≈ 0.22 obtained for RS. The lamellar thickness for URS increases with increase of MWCNT content and that for RS decreases, as evaluated from both differential thermal analyses and X‐ray diffraction data. Copyright © 2011 Society of Chemical Industry 相似文献
10.
This paper proposes the “Substitute Interface” to utilize the flat surfaces of objects around us as part of an ad hoc mobile device. The substitute interface is established by the combination of wearable devices such as a head-mounted display with camera and a ring-type microphone. The camera recognizes which object the user intends to employ. When the user picks up and taps the object, such as a notebook, a virtual display is overlaid on the object, and the user can operate the ad hoc mobile device as if the object were part of the device. Display size can be changed easily by selecting a larger object. The user’s pointing/selection action is recognized by the combination of the camera and the ring-type microphone. We first investigate the usage scene of tablet devices and create a prototype that can operate as a tablet device. Experiments on the prototype confirm that the proposal functions as intended. 相似文献