Field Performance of High Oleic Soybeans with Mutant FAD2-1A and FAD2-1B Genes in Tennessee

Soybean [Glycine max (L.) Merr.] oil with high oleic acid (>75%) has increased oxidative stability and health benefits that are valuable for food, fuel, and industrial products. It has been determined that two naturally occurring mutations in genes FAD2-1A and FAD2-1B can combine to produce high oleic soybeans. The objective of this study was to test the effect of these mutant alleles on seed yield and oil and protein concentration. Molecular markers assisted in the creation of a population of 48 BC₃F_2:4 lines (93.75% expected genome commonality). Each line was classified into one of four genotypic groups where both FAD2-1A and FAD2-1B genes were either homozygous wild type or mutant, respectively. Twelve lines for each genotypic group were evaluated in three replications at six locations across Tennessee. There was no seed yield difference between the high oleic genotypic group and the other groups (P < 0.05). On the other hand, there were differences in fatty acid profiles and oil and protein concentrations. In combination, the mutant FAD2-1A and FAD2-1B alleles produced a mean of 803.1 g kg⁻¹ oleic acid. This is, on average, approximately 500 g kg⁻¹ more oleic acid compared to soybean lines with only one mutant FAD2-1 allele. The high oleic double mutant group had more total oil (228.0 g kg⁻¹) and protein (401.0 g kg⁻¹) compared to all other genotypic groups (P < 0.05). Overall, this specific combination of mutant FAD2-1A and FAD2-1B alleles appears to generate conventional high oleic soybeans without a yield drag.     

Dietary exposure of the Belgian population to emulsifiers E481 (sodium stearoyl-2-lactylate) and E482 (calcium stearoyl-2-lactylate)

A dietary exposure assessment of food emulsifiers E481 (sodium stearoyl-2-lactylate) and E482 (calcium stearoyl-2-lactylate) in the Belgian population was performed. Nationally representative food consumption data from the Belgian National Food Consumption Surveys 2004 (BNFCS2004) and 2014 (BNFCS2014) were used for calculations. A conservative approach (combining individual food consumption data with the maximum permitted level (MPL) of foods (tier 2), was compared with more refined estimates (combining individual food consumption data with actual concentrations measured in food products available on the Belgian market (tier 3)). Estimated daily intakes were compared to the acceptable daily intake (ADI) of the stearoyl-2-lactylates. The results of tier 2 demonstrated that 92% of the children (3–9 years), 53% of the adolescents (10–17 years), 15% of the adults (18–64 years) and 26% of the elderly (64–98 years) had a potential intake higher than the ADI. When replacing the MPL with maximum analysed concentration levels in foods, daily intake estimates decreased dramatically. The estimated daily intake of the food emulsifiers was below the ADI for all age groups, except for a small percentage of children (1.9%) for which the intake exceeded the ADI. The main contributors to the exposure of E481 and E482 were bread, rolls and fine bakery wares.     

Effect of gamma irradiation on structural,morphological and optical properties of thermal spray pyrolysis deposited CuO thin film

This study reports the influences of gamma irradiation (GI) in the range of 20–100 kGy on CuO thin films via thermal spray pyrolysis technique on the glass substrates. The results demonstrate significant influences of GI on the crystallographic, microstructural and optical characteristics of CuO thin films. The obtained XRD results showed that the crystallinity of the films deteriorates by gradually decreasing crystallite size (from 59.13 to 46 nm) as applied gamma doses increases. However, the basic monoclinic crystal structure remains same. The dislocation density and lattice strain increased with the rise of GI absorbed dose due to the creation of defects. The values of number of crystallites per unit surface area increased as dose increased indicating the abundance of crystallization of nano CuO thin films. A UV–Vis–NIR spectrophotometer was utilized to determine the optical properties and obtained results indicated that the optical energy band gap (OBG) energies reduced from 2.00 to 1.72 eV as the doses increased from 0 to 100 kGy. No distinctions of the monoclinic phase of virgin CuO thin film have been perceived under applied absorbed doses, notwithstanding the slight deterioration of the crystallinity and narrowing the OBG.     

Influence of high power ultrasound on physical–chemical properties of polypropylene films aimed for food packaging: barrier and contact angle features

Investigation was focused on the impact of high power ultrasound (HPUS), also called thermosonication, on the oxygen permeation properties (permeability, solubility and diffusion coefficients) of barrier films aimed for food packaging. For this purpose, biaxially oriented polypropylene (BOPP) coated with acrylic/polyvinylidene chloride (BOPPAcPVDC) and biaxially oriented coextruded polypropylene (BOPPcoex) were used. The physical–chemical profile of the samples was determined using goniometry. There is a significant impact only of extreme HPUS conditions (the longest time and the highest amplitude) on the permeability, solubility and diffusion coefficients of oxygen through the BOPP films. The highest influence on the oxygen permeability in both investigated BOPP samples involved an HPUS with an amplitude of 100% during a 6 min treatment. However, BOPP samples showed different sensitivities at lower HPUS treatments. © 2017 Society of Chemical Industry     

The Impact of Zeta Potential and Physicochemical Properties of TiO2‐Based Nanocomposites on Their Biological Activity

The aim of this research was to observe the relationship between zeta potential, morphology, surface area, porosity, chemical composition, and ecotoxicity of nanocomposite powders such as Au/TiO₂, Ag₂O/TiO₂, PdO/TiO₂, Ag/TiO₂/SiO₂, Ag/N(C)TiO₂, and SiO₂/TiO₂ from which Ag₂O/TiO₂, Ag/N(C)/TiO_2, and Ag/TiO₂/SiO₂ were exhibiting good antimicrobial properties. It was observed, that nanomaterials characterized by similar morphology and zeta potential revealed the similar toxic behavior. The samples of higher agglomeration and higher zeta potential, especially Ag/TiO₂/SiO₂ xerogel and TiO₂/SiO₂ aerogel were generally less ecotoxic to water organisms and plants. They were also not genotoxic in concentrations up to 500 and 250 mg/L, respectively.     

Setting Up a Numerical Model of a DAF Tank: Turbulence, Geometry, and Bubble Size

This paper discusses the modeling framework and identifies a number of parameters relevant when setting up a computational fluid dynamics simulation of a dissolved air flotation (DAF) tank. The selection of a turbulence model, the choice between performing two-dimensional (2D) or three-dimensional (3D) simulations, the effects of the design of the flow geometry and the influence of the size of the air bubbles are addressed in the paper. The two-phase flow of air and water is solved in the Eulerian-Lagrangian frame of reference. The realizable k-ε model with nonequilibrium wall functions is suggested as a compromise between a need to effectively resolve the flow and the cost of the simulations. There is a discussion on the conditions for which the steady-state simulations are appropriate. We demonstrate that a steady 2D model can simulate a stratified flow pattern. Our results show that 2D models require adjustments in geometry (e.g., substitution of the outlet pipes to an outlet distributed over the total width of the tank) and in the parameters governing the flow in order to account for the true 3D nature of some of the flow patterns. In addition, we show that the bubble size has a larger influence on the flow in the separation zone than in the contact zone.