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排序方式: 共有425条查询结果,搜索用时 171 毫秒
1.
Jiwon Lee Bryan S. Der Christos S. Karamitros Wenzong Li Nicholas M. Marshall Oana I. Lungu Aleksandr E. Miklos Jianqing Xu Tae Hyun Kang Chang-Han Lee Bing Tan Randall A. Hughes Sang Taek Jung Gregory C. Ippolito Jeffrey J. Gray Yan Zhang Brian Kuhlman George Georgiou Andrew D. Ellington 《American Institute of Chemical Engineers》2020,66(3):e16864
We used the molecular modeling program Rosetta to identify clusters of amino acid substitutions in antibody fragments (scFvs and scAbs) that improve global protein stability and resistance to thermal deactivation. Using this methodology, we increased the melting temperature (Tm) and resistance to heat treatment of an antibody fragment that binds to the Clostridium botulinum hemagglutinin protein (anti-HA33). Two designed antibody fragment variants with two amino acid replacement clusters, designed to stabilize local regions, were shown to have both higher Tm compared to the parental scFv and importantly to retain full antigen binding activity after 2 hr of incubation at 70°C. The crystal structure of one thermostabilized scFv variants was solved at 1.6 Å and shown to be in close agreement with the RosettaAntibody model prediction. 相似文献
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Kudashev S. V. Kondrasenko A. A. Maiulev A. N. Babkin V. A. Belousova V. S. Andreev D. S. Zheltobryukhov V. F. Kuznetsova N. V. 《Fibre Chemistry》2022,53(5):291-295
Fibre Chemistry - Modification of polycaproamide by 1H,1H,13H-trihydroperfluorotridecan-1-ol immobilized on montmorillonite produced an F-containing polymer composite. The structure of the... 相似文献
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Microwave Sol–Gel Synthesis of CaGd2(MoO4)4:Er3+/Yb3+ Phosphors and Their Upconversion Photoluminescence Properties 下载免费PDF全文
Chang Sung Lim Victor Atuchin Aleksandr Aleksandrovsky Maxim Molokeev Aleksandr Oreshonkov 《Journal of the American Ceramic Society》2015,98(10):3223-3230
CaGd2(MoO4)4:Er3+/Yb3+ phosphors with the doping concentrations of Er3+ and Yb3+ (x = Er3+ + Yb3+, Er3+ = 0.05, 0.1, 0.2, and Yb3+ = 0.2, 0.45) have been successfully synthesized by the microwave sol–gel method, and the crystal structure refinement and upconversion photoluminescence properties have been investigated. The synthesized particles, being formed after heat‐treatment at 900°C for 16 h, showed a well‐crystallized morphology. Under the excitation at 980 nm, CaGd2(MoO4)4:Er3+/Yb3+ particles exhibited strong 525 and 550‐nm emission bands in the green region and a weak 655‐nm emission band in the red region. The Raman spectrum of undoped CaGd2(MoO4)4 revealed about 15 narrow lines. The strongest band observed at 903 cm?1 was assigned to the ν1 symmetric stretching vibration of MoO4 tetrahedrons. The spectra of the samples doped with Er and Yb obtained under 514.5 nm excitation were dominated by Er3+ luminescence preventing the recording Raman spectra of these samples. Concentration quenching of the erbium luminescence at 2H11/2→4I15/2 and 4S3/2→4I15/2 transitions in the CaGd2(MoO4)4:Er3+/Yb3+ crystal structure was established to be approximately at the 10 at.% doping level. 相似文献
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Alexander Pyattaev Jiri Hosek Kerstin Johnsson Radko Krkos Mikhail Gerasimenko Pavel Masek Aleksandr Ometov Sergey Andreev Jakub Sedy Vit Novotny Yevgeni Koucheryavy 《ETRI Journal》2015,37(5):877-887
This paper is a first‐hand summary on our comprehensive live trial of cellular‐assisted device‐to‐device (D2D) communications currently being ratified by the standards community for next‐generation mobile broadband networks. In our test implementation, we employ a full‐featured 3GPP LTE network deployment and augment it with all necessary support to provide real‐time D2D connectivity over emerging Wi‐Fi‐Direct (WFD) technology. As a result, our LTE‐assisted WFD D2D system enjoys the required flexibility while meeting the existing standards in every feasible detail. Further, this paper provides an account on the extensive measurement campaign conducted with our implementation. The resulting real‐world measurements from this campaign quantify the numerical effects of D2D functionality on the resultant system performance. Consequently, they shed light on the general applicability of LTE‐assisted WFD solutions and associated operational ranges. 相似文献
7.
D.A. Belov I.V. Makarenko A.V. Dunaev A.V. Babkin A.V. Solopchenko M.Y. Yablokova A.V. Kepman A.V. Tretyakov A.V. Ulyanov A.G. Gromashev 《Polymer Composites》2016,37(7):2252-2259
Thermal processes of carbon fiber‐reinforced composite parts curing cycle were studied experimentally and by mathematical simulation. Flat panel, T‐stringer, and five‐stringer detail based on tetrafunctional MY721 epoxy resin were investigated. Application of standard curing cycle (heating followed by isothermal exposure at 180°C) leads to the overheating of 28°C in flat panel and of 30°C in T‐stringer of 24 mm thickness. The model considers the vacuum bag with auxiliary materials as separate layers. This approach allows to simulate the shifting of thermal field to the vacuum bag side. Simulation results show good agreement with the experimentally observed temperature fields. The model helps to optimize the curing cycle to reduce the local overheating. The same temperature regime could be used for all three geometries. Therefore, the optimization can be carried out only for the simple‐shape parts to save the calculation time and simplify experiments at the stage of model testing. POLYM. COMPOS., 37:2252–2259, 2016. © 2015 Society of Plastics Engineers 相似文献
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ABRAMOV Aleksandr 《石油勘探与开发》2019,(3)
改进并测试了包含30多个钻井工程参数的技术-经济模型,通过新开发的计算机程序列出平台上的井丛分组方式并计算其技术经济效益。针对含24口初始产油量不同的井的平台,研究分析了7种钻井方案,得出了3种折现率和5种产油量递减率条件下净现值(NPV)最优的分组方式。研究表明,针对井丛井数不等的井平台,可能只需对现有设计进行轻微调整(改变几个井丛的布局)就能得到最大净现值;相对净现值增幅与净现值的绝对值成反比;相比常规项目中具有相等井数的井丛,采用井丛井数不等的分组方式可以使NPV增幅达到1%,页岩地层开发项目NPV增幅可达2%或更多,边际油田项目增幅高达45%。图9表4参18 相似文献
9.
Nikulina A. A. Smirnov A. I. Turichin G. A. Klimova-Korsmik O. G. Babkin K. D. 《Metal Science and Heat Treatment》2017,59(7-8):534-539
Metal Science and Heat Treatment - The structure of laser-welded joints of parts having different thicknesses fabricated from alloys based on aluminum and titanium has been studied. Results of... 相似文献
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