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1.
Sobha Karuthedom George Lucia Laukov Ren Weiss Vladislav Semak Birgit Fendl Victor U. Weiss Stephanie Steinberger Günter Allmaier Carla Tripisciano Viktoria Weber 《International journal of molecular sciences》2021,22(8)
Growing interest in extracellular vesicles (EVs) has prompted the advancements of protocols for improved EV characterization. As a high-throughput, multi-parameter, and single particle technique, flow cytometry is widely used for EV characterization. The comparison of data on EV concentration, however, is hindered by the lack of standardization between different protocols and instruments. Here, we quantified EV counts of platelet-derived EVs, using two flow cytometers (Gallios and CytoFLEX LX) and nanoparticle tracking analysis (NTA). Phosphatidylserine-exposing EVs were identified by labelling with lactadherin (LA). Calibration with silica-based fluorescent beads showed detection limits of 300 nm and 150 nm for Gallios and CytoFLEX LX, respectively. Accordingly, CytoFLEX LX yielded 40-fold higher EV counts and 13-fold higher counts of LA+CD41+ EVs compared to Gallios. NTA in fluorescence mode (F-NTA) demonstrated that only 9.5% of all vesicles detected in scatter mode exposed phosphatidylserine, resulting in good agreement of LA+ EVs for CytoFLEX LX and F-NTA. Since certain functional characteristics, such as the exposure of pro-coagulant phosphatidylserine, are not equally displayed across the entire EV size range, our study highlights the necessity of indicating the size range of EVs detected with a given approach along with the EV concentration to support the comparability between different studies. 相似文献
2.
Felipe A. Perdomo Siti H. Khalit Claire S. Adjiman Amparo Galindo George Jackson 《American Institute of Chemical Engineers》2021,67(3):e17194
The SAFT-γ Mie group-contribution equation of state is used to represent the fluid-phase behavior of aqueous solutions of a variety of linear, branched, and cyclic amines. New group interactions are developed in order to model the mixtures of interest, including the like and unlike interactions between alkyl primary, secondary, and tertiary amine groups (NH2, NH, N), cyclic secondary and tertiary amine groups (cNH, cN), and cyclic methine-amine groups (cCHNH, cCHN) with water (H2O). The group-interaction parameters are estimated from appropriate experimental thermodynamic data for pure amines and selected mixtures. By taking advantage of the group-contribution nature of the method, one can describe the fluid-phase behavior of mixtures of molecules comprising those groups over broad ranges of temperature, pressure, and composition. A number of aqueous solutions of amines are studied including linear, branched aliphatic, and cyclic amines. Liquid–liquid equilibria (LLE) bounded by lower critical solution temperatures (LCSTs) have been reported experimentally and are reproduced here with the SAFT-γ Mie approach. The main feature of the approach is the ability not only to represent accurately the experimental data employed in the parameter estimation, but also to predict the vapor–liquid, liquid–liquid, and vapor–liquid–liquid equilibria, and LCSTs with the same set of parameters. Pure compound and binary phase diagrams of diverse types of amines and their aqueous solutions are assessed in order to demonstrate the main features of the thermodynamic and fluid-phase behavior. 相似文献
3.
Muniyandi Kasipandi Jagadeesan Gayathri George Blassan P. Manoharan Ashwini Lydia Nataraj Gayathri Abrahamse Heidi Thangaraj Parimelazhagan 《Food science and biotechnology》2022,31(13):1717-1728
Food Science and Biotechnology - A rich source of nutrients, figs have a number of clinically validated benefits. This study aimed to evaluate the in vitro simulated gastrointestinal digestion, and... 相似文献
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5.
Aikaterini Teknetzi Evangelia Tarani Elli Symeou Dimitrios Karfaridis Dimitrios Stathokostopoulos Eleni Pavlidou Theodora Kyratsi Evripidis Hatzikraniotis Konstantinos Chrissafis George Vourlias 《Ceramics International》2021,47(1):243-251
Higher manganese silicides (HMS) are promising alternative materials for middle to high temperature thermoelectric applications as a low-cost, non-toxic and highly stable p-type leg. Many of the preparation methods that have been reported previously require long-time and energy consuming processes, as well as expensive equipment, and often do not result in a material of sufficient quality. In this study, the simple, cost-effective and eco-friendly technique of pack cementation is applied. HMS powders synthesized at different experimental conditions are studied and compared considering their structure, composition, short-term thermal stability in air and thermoelectric properties. X-ray diffraction analysis, X-ray photoelectron spectroscopy, scanning electron microscopy, thermogravimetry and thermoelectric measurements (in terms of Seebeck coefficient, electrical and thermal conductivity) were employed for the characterization of the material and evaluation of its performance. All samples were identified as HMS and only some negligible traces of MnSi were detected. They moderately oxidize when heated non-isothermally under air atmosphere up to 1473 K, while the presence of HMS remains dominant even at such high temperatures. Their thermoelectric properties were remarkable for an undoped material, with a maximum figure of merit (ZT) of 0.47 at 777 K. Pack cementation appeared to have a great potential as the synthesis route of high-efficiency HMS. 相似文献
6.
Elena Hadjimbei George Botsaris Vlasios Goulas Eleni Alexandri Vassilis Gekas Ioannis P Gerothanassis 《International Journal of Dairy Technology》2020,73(1):134-143
The present study describes the development of a functional goats' milk yoghurt, using Pistacia atlantica resin extracts and Saccharomyces boulardii, in an attempt to combine the beneficial effects of the milk, extract and probiotic microorganisms. Results demonstrated that Pistacia extracts promoted the survival of lactic acid bacteria and NMR spectra additionally revealed that the functional fatty acids can be retained during the shelf life of yoghurts, alongside with the other bioactive compounds. The organoleptic assessment revealed that there were no significant differences in flavour, body, texture and appearance between the different yoghurts. 相似文献
7.
Bruna R. Loiola George S. Dulikravich 《Numerical Heat Transfer, Part A: Applications》2018,73(10):685-701
This study presents the numerical solution of a bioheat transfer problem related to the ablation of biological tissues continuously heated by a laser. Different mechanisms of thermal decomposition, such as coagulation, vaporization, and removal of the necrotic tissues, were taken into account in the analysis. The finite volume method was used to obtain the numerical solution of the temperature field, while the volume of fluid method was used to track the ablation interface. Results obtained for cases of practical interest provide a good indication of the area affected by the thermal treatment and are in good agreement with experimental data available in the literature. 相似文献
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9.
The combined effect of particulate fouling and magnetic field on the efficiency of a convective–radiative porous fin heatsink with temperature‐dependent thermal conductivity is presented. The developed thermal models are solved using differential transformation method. The effects of thermal conductivity, porosity, convection, radiation parameter, and thermal fouling number on the fin thermal efficiency are investigated. The presence of thermal fouling on the surface of the fin is shown to increase the temperature distribution. The presence of particle deposition on the fin surface significantly decreases the rate of heat transfer as additional thermal resistance of the fouling layer decreases the thermal performance of porous fin heatsink. Moreover, the fin efficiency decreases as the value of fouled Biot, Darcy, radiation number, and thermogeometric parameter increases. It is established that Mf < Mc, which indicates that the efficiency of the fouled fin is greater than the efficiency of the clean fin. Furthermore, the result of the present study is validated with the established results of Chebyshev spectral collocation method and fourth‐order Runge–Kutta with shooting method and an error margin of 0.000000023 is established. 相似文献
10.
Suying Xu Adam C. Sedgwick Souad A. Elfeky Wenbo Chen Ashley S. Jones George T. Williams A. Toby A. Jenkins Steven D. Bull John S. Fossey Tony D. James 《Frontiers of Chemical Science and Engineering》2020,14(1):112
A boronic acid-based anthracene fluorescent probe was functionalised with an acrylamide unit to incorporate into a hydrogel system for monosaccharide detection. In solution, the fluorescent probe displayed a strong fluorescence turn-on response upon exposure to fructose, and an expected trend in apparent binding constants, as judged by a fluorescence response where D-fructose>D-galactose>D-mannose>D-glucose. The hydrogel incorporating the boronic acid monomer demonstrated the ability to detect monosaccharides by fluorescence with the same overall trend as the monomer in solution with the addition of D-fructose resulting in a 10-fold enhancement (≤0.25 mol/L). 相似文献