康氏木霉固体发酵生产纤维素酶优化研究--培养基用料与工艺条件优化

培养基及培养条件的优化是降低酶制剂成本、提高酶活、实现其工业化生产的重要措施。本研究采用均匀设计U15(5^8)和双温度培养法(前30h恒温30℃，后续恒温27℃)进行康氏木霉产酶固体发酵生产纤维素酶，对滤纸酶、羧甲基纤维素酶、棉花酶、β-萄糖苷酶的活力进行回归分析，而后对各酶活方程求和得到纤维素酶的总活力回归方程并进行约束规划求解。结果表明：应用双温度培养法进行康氏木霉固体发酵生产纤维素酶时，在自然补给氧气，培养基pH自然(约6．5)，并保持环境湿度约60％的条件下，72h是适宜的发酵周期：培养基构成以稻草粉50％，(NH4)2SO48％，麸皮42％，加水量以4．9倍的固体物质为宜；少量的Tween80有利于纤维素酶活力的提高。     

潜流人工湿地的局限性与运行问题

针对目前在潜流人工湿地技术认识上的一些分歧，从湿地植物的输氧能力、人工湿地脱氮除磷能力和设计参考依据等方面，探讨了人工湿地的应用局限性，总结了湿地运行中存在的问题及解决措施，指出了深入研究潜流人工湿地处理机制的必要性。     

Proteome Analysis of Inhibitory Effect of Gadolinium on Sinorhizobium fredii

The inhibitory effect of gadolinium on Sinorhizobium fredii USDA 205 was studied on a global scale using twodimensional gel electrophoresis and MALDI-TOF MS. The results indicated that 22 proteins were significantly affected by 1 mmol · L^-1 Gd^3 ＋ treatment when compared with an untreated control. Among these proteins, nine were up-regulated and thirteen were down-regulated. The differently expressed proteins were classified into 8 functional categories based on their functions, including transporters, proteins for cellular defence, and proteins involved in metabolism.