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1.
Cellular Antisense Activity of PNA–Oligo(bicycloguanidinium) Conjugates Forming Self‐Assembled Nanoaggregates 下载免费PDF全文
Dr. Julián Valero Dr. Takehiko Shiraishi Prof. Javier de Mendoza Prof. Peter E. Nielsen 《Chembiochem : a European journal of chemical biology》2015,16(11):1593-1600
A series of peptide nucleic acid–oligo(bicycloguanidinium) (PNA–BGn) conjugates were synthesized and characterized in terms of cellular antisense activity by using the pLuc750HeLa cell splice correction assay. PNA–BG4 conjugates exhibited low micromolar antisense activity, and their cellular activity required the presence of a hydrophobic silyl terminal protecting group on the oligo(BG) ligand and a minimum of four guanidinium units. Surprisingly, a nonlinear dose–response with an activity threshold around 3–4 μM , indicative of large cooperativity, was observed. Supported by light scattering and electron microscopy analyses, we propose that the activity, and thus cellular delivery, of these lipo‐PNA–BG4 conjugates is dependent on self‐assembled nanoaggregates. Finally, cellular activity was enhanced by the presence of serum. Therefore we conclude that the lipo‐BG‐PNA conjugates exhibit an unexpected mechanism for cell delivery and are of interest for further in vivo studies. 相似文献
2.
Ultrasonic welding of mild steel sheet (SPCC) and aluminium alloy sheet (A5052) was conducted using two different weld tips of a knurled tip (K-tip) and a curved shape tip without knurled edges (C-tip). Behaviours of the weld tip and the welded materials during the weld process were analysed using a high-speed camera observation and an image correlation. The C-tip welding shows a completely different displacement behaviour from the K-tip welding; a relative motion between the tool tip and A5052 in contact with the tip predominantly occurs in the C-tip in contrast to a relative motion between A5052 and SPCC observed in the K-tip. The joint strength using the C-tip was higher than that using the K-tip under the weld conditions in this study. It is demonstrated that the relative motion behaviour in the C-tip causes a macroscopic deformation of A5052 in the vibration direction. This phenomenon promotes growth of a bonded region, resulting in the increase of joint strength. It is surmised that Fe–Al intermetallic layer forms at the weld interface, and the joint strength consequently decreases for the longer weld time condition. 相似文献
3.
Regime shifts observed in Lake Kasumigaura,a large shallow lake in Japan: Analysis of a 40‐year limnological record 下载免费PDF全文
Lake Kasumigaura, which is composed of the two basins (Nishiura and Kitaura), is a large, shallow, hypereutrophic lake. Phytoplankton and water quality records from the past forty years were analysed to elucidate whether or not, when, and what type of certain regime shifts may have occurred, based on using inferential regime shift detectors. Characteristics of the phytoplankton and water quality changes were similar at 6 sampling sites in the two basins, with 20 water quality parameters being classified into four groups, based on cluster analysis. Shifts in dominant plankton groups (DPGs) and water quality occurring almost concomitantly, concentration on the period from 1987 to 1992 (Shift A) and from 1997 to 2001 (Shift B), with those observed for the two basins usually being similar with small differences. Two types of inferential regime shift detectors (sequential t‐test type; Rodionov's RSD and sequential F‐test type: package strucchange in R) yielded similar timings and significances of the shifts. Furthermore, changes in skewness and conditional heteroskedasticity (package early warnings in R) usually represented early warning signals before the shifts. Correlation analysis and ratios of dissolved inorganic nitrogen (DIN) vs. total phosphorus (TP) supported the hypothesis that phosphorus was the phytoplankton biomass limiting nutrient, except for one period for the Nishiura basin. Neither the nitrogen:phosphorus (N/P) ratio hypothesis and ammonia–nitrate (NH4‐N/NO3‐N) hypothesis satisfactorily explain the DPGs before and after Shift A (Microcystis spp. and Planktothrix spp., respectively), although it may be possible that these ratios triggered the DPG change in this shift. A considerable increase in silicon was observed for Shift B when the DPGs changed from cyanobacteria to diatoms. Further studies on the accurate types and triggers of the regime shifts are necessary to better understand the interactions between ecosystem and water quality for this and similar lakes elsewhere. 相似文献
4.
Satya Sekhar Bhogilla Hiroshi Ito Takehiko Segawa Atsushi Kato Akihiro Nakano 《International Journal of Hydrogen Energy》2017,42(19):13827-13838
The renewable energy source like wind energy generates electric power with intermittent nature. Hydrogen energy system can help to solve the fluctuation problem of the wind power. Totalized Hydrogen Energy Utilization System (THEUS) consists of a Unitized Reversible Fuel Cell (URFC), a hydrogen storage tank, and other auxiliary components. Wind power is inherently variable; the URFC will be subjected to a dynamic input power profile in water electrolyzer mode operation. This paper describes the THEUS operation and performance at different variations in intermittent wind power. The performance of the THEUS was evaluated in water electrolyzer and fuel cell mode operation. The stack efficiency, system efficiency, and system efficiency including heat output from the URFC were presented at each operation. The total efficiency of the URFC and THEUS were also investigated. The maximum total efficiency of the URFC and THEUS were 53% and 66%, respectively. 相似文献
5.
We present numerically verified a posteriori estimates of the norms of inverse operators for linear parabolic differential equations. In case that the corresponding elliptic operator is not coercive, existing methods for a priori estimates of the inverse operators are not accurate and, usually, exponentially increase in time variable. We propose a new technique for obtaining the estimates of the inverse operator by using the finite dimensional approximation and error estimates. It enables us to obtain very sharp bounds compared with a priori estimates. We will give some numerical examples which confirm the actual effectiveness of our method. 相似文献
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Background
Titanium dioxide (TiO2) nanoparticles and fullerene (C60) are two attractive manufactured nanoparticles with great promise in industrial and medical applications. However, little is known about the genotoxic response of TiO2 nanoparticles and C60 in mammalian cells. In the present study, we determined the mutation fractions induced by either TiO2 nanoparticles or C60 in gpt delta transgenic mouse primary embryo fibroblasts (MEF) and identified peroxynitrite anions (ONOO-) as an essential mediator involved in such process. 相似文献9.
Interactive and autonomous agents might be common in everyday life in the future; we expect that such agents will have the
ability to communicate with people naturally. For natural communication, the agents should speculate about the intentions
of the people they interact with. To enable agents to speculate about intentions like deception, we focused on unconscious
expressions when people tell a lie. However, there is no system that can meet the necessary conditions for measuring nonverbal
information in natural communication. Therefore, we made a real-time system for measuring gaze direction and facial features.
We conducted experiments for discriminating lies by using the system in a situation similar to actual communication. As a
result, we found that we could discriminate lies by using diverse nonverbal information in the same way people did. 相似文献
10.
Takehiko Watanabe Masataka ShirokiAtsushi Yanagisawa Tomohiro Sasaki 《Journal of Materials Processing Technology》2010,210(12):1646-1651
Authors investigated the effect of ultrasonic vibration on the solidification microstructure and mechanical properties of the weld metal of ferritic stainless steel by introducing directly ultrasonic vibration into the weld molten pool using ultrasonically vibrating filler metal. The main results obtained in this study are as follow. 相似文献