An automated, parallel processing approach to biomolecular sample preparation

Sample preparation for DNA and RNA assays is a prime candidate for laboratory automation. A novel, parallel processing device that performs the three separate liquid-handling functions necessary for such sample preparation-dispensing, pipetting, and pressurizing-is presented. The device comprises an array of fine nozzles connected by fluidic channels to automatically and precisely distribute flow between one source and an array of points. The design principles, as well as the experimental and computational methods used to develop the device, are described. Test results, including accuracy, uniformity, volume range, and timing, are presented. The functionality of the device is demonstrated by performing a solid-phase extraction of DNA with two types of microcolumns.     

Questionnaire development: an examination of the Nordic Musculoskeletal questionnaire

This paper describes the outcome of user trials of the Nordic Musculoskeletal Questionnaire which encompassed the views of the following groups: data entry clerks, technical staff, administrative clerks and 481 subjects employed in 10 supermarkets. A significant number of improvements was identified, especially concerning its wording, layout and administration. This has led to a standardized version being produced for use in studying the prevalence of reported symptoms in many types of occupational groups.     

Biochemical,functional, and histochemical effects of essential fatty acid deficiency in rat kidney

The present study was designed to examine the effects of EFA deficiency (EFAD) on biochemical, functional, and structural aspects of the kidney in growing and adult rats fed a normal or EFAD diet for 9 wk after weaning. Food and fluid intake (FI), urine volume, and Na⁺ and K⁺ excretions were measured weekly from weeks 4 to 8 by placing the rats in individual metabolic cages for 24 h. At week 9, Li⁺ and a 5% water load, respectively, were administered at 14 and 1.5 h prior to glomerular and proximal tubular function studies, as assessed by 3-h creatinine (C_Cr) and Li⁺ (C_Li+) clearances. Hematocrit and urine volume; serum and urine [Cr], [Li⁺], [Na⁺], and [K⁺]; and renal FA distribution were also measured. Data [corrected to 100 g/body weight (bw) and presented as means ±SEM] were significant, at P<-0.05. Despite a similar ingestion of solids from weeks 4 to 7 (weeks 7 to 10 of life), the rats on the EFAD diet showed a decreased body weight from week 5. From weeks 4 to 8, Fl and urine volume were similar for both groups, but the Fl increased at week 6 in the EFAD group; 24-h Na⁺ and K⁺ excretions were similar at all weeks, except for an increase in the EFAD group for both ions at week 7. In the EFAD group, C_Cr and C_Li+ decreased by 27 and 56.3%, respectively (385.7±33.4 vs. 280±21.1, and 21.0±2.1 vs. 9.2±1.1 μL/min/100 g; n=9 vs. 10), the latter result suggesting increased proximal reabsorption. The 3-h Na⁺ and K⁺ excretions were similar, but the Li⁺ decreased (0.78±0.06×10⁻² vs. 0.32±0.03×10⁻² μeq/min/100 g) in the EFAD group, giving additional support to the suggestion. Renal structure was normal and similar for both groups, but the EFAD group showed a more prominent proximal tubule brush border, together with heavier periodic acid-Schiff staining in all specimens from weeks 5 to 9. In the EFAD group, FA of the n−9 and n−7 series were higher, but most of the n−6 series were lower as a percentage of total lipids in the medulla and cortex. Medullary levels of 20∶4n−6 were maintained, 22∶4n−6 declined twice, arachidonic acid was maintained, and 20∶5n−3 was lower. The EFAD diet affected glomerular function, proximal tubular structure and function, and FA distribution in the rat kidney.     

Structural, electronic, and magnetic properties of heterofullerene C(58)Si with odd number of atoms and a near planar tetracoordinate Si atom

Density functional calculations and minimization techniques have been employed to characterize the structural and electronic properties of [5,6]-heterofullerene-C₅₈Si-C_2v. Since it has odd number of atoms and a near planar tetracoordinate Si atom on the skeleton of the cage, it has odd number of atoms assembling a cage and is a novel molecule. Vibrational frequencies of the molecule have been calculated at the B3LYP/6-31G* level of theory. The absence of imaginary vibrational frequency confirms that the molecule corresponds to a true minimum on the potential energy hypersurface. Sixteen ¹³C nuclear magnetic resonance (NMR) spectral signals of C₅₈Si are characterized, and its heat of formation was estimated in this work.     

Influence of age and proximity warning devices on collision avoidance in simulated driving

OBJECTIVE: We conducted a set of experiments to examine the utility of several different uni- and multimodal collision avoidance systems (CASs) on driving performance of young and older adult drivers in a high-fidelity simulator. BACKGROUND: Although previous research has examined the efficacy of different CASs on collision avoidance, there has been a dearth of studies that have examined such devices in different driving situations with different populations of drivers. METHOD: Several different CAS warnings were examined in varying traffic and collision configurations both without (Experiment 1a) and with (Experiment 2) a distracting in-vehicle task. RESULTS: Overall, collision avoidance performance for both potential forward and side object collisions was best for an auditory/visual CAS, which alerted drivers using both modalities. Interestingly, older drivers (60-82 years of age) benefited as much as younger drivers from the CAS, and sometimes they benefited more. CONCLUSION: These data suggest that CASs can be beneficial across a number of different driving scenarios, types of collisions, and driver populations. APPLICATION: These results have important implications for the design and implementation of CASs for different driver populations and driving conditions.     

A framework for weighted fusion of multiple statistical models of shape and appearance

This paper presents a framework for weighted fusion of several active shape and active appearance models. The approach is based on the eigenspace fusion method proposed by Hall et al., which has been extended to fuse more than two weighted eigenspaces using unbiased mean and covariance matrix estimates. To evaluate the performance of fusion, a comparative assessment on segmentation precision as well as facial verification tests are performed using the AR, EQUINOX, and XM2VTS databases. Based on the results, it is concluded that the fusion is useful when the model needs to be updated online or when the original observations are absent