Understanding the metabolic burden of recombinant antibody production in Saccharomyces cerevisiae using a quantitative metabolomics approach

The cellular changes induced by heterologous protein expression in the yeast Saccharomyces cerevisiae have been analysed on many levels and found to be significant. However, even though high‐level protein production poses a metabolic burden, evaluation of the expression host at the level of the metabolome has often been neglected. We present a comparison of metabolite profiles of a wild‐type strain with those of three strains producing recombinant antibody variants of increasing size and complexity: an scFv fragment, an scFv–Fc fusion protein and a full‐length IgG molecule. Under producing conditions, all three recombinant strains showed a clear decrease in growth rate compared with the wild‐type strain and the severity of the growth phenotype increased with size of the protein. The levels of 76 intracellular metabolites were determined using a targeted (semi) quantitative mass spectrometry based approach. Based on unsupervised and supervised multivariate analysis of metabolite profiles, together with pathway activity profiling, the recombinant strains were found to be significantly different from each other and from the wild‐type strain. We observed the most prominent changes in metabolite levels for metabolites involved in amino acid and redox metabolism. Induction of the unfolded protein response was detected in all producing strains and is considered to be a contributing factor to the overall metabolic burden on the cells.     

Spatially Resolved Characterization of the Gas Propagator in Monolithic Structured Catalysts Using NMR Diffusiometry

Gas diffusivity measurements in opaque porous media were performed using nuclear magnetic resonance. An optimized pulsed‐field gradient stimulated echo method with free volume selection was used to investigate the propagator of thermally polarized methane gas within commercial monolithic catalyst supports. Since signal losses due to T₂ relaxation were minimized by using a short echo time, diffusion processes could be characterized by the measured propagator functions and effective diffusion coefficients were determined for a broad range of observation times and in different spatial directions. The study of this noninvasive characterization of gas diffusion found a clear effect of the monolith type and its pore size and coating on the effective gas diffusion coefficient and the apparent tortuosity for a given observation time.     

To look or not to look: An eye movement study of hypervigilance during change detection in high and low spider fearful students.

Previous eye movement studies of attentional bias in spider fear reported inconsistent results with respect to early attentional capture, suggesting that overt attentional capture only reliably occurs under specific circumstances. In addition, none of these studies explored covert attention. The present study examined attentional bias in spider phobia using a change detection paradigm that was expected to provide good conditions for documenting attentional capture. In contrast to our expectations, eye movement data showed that all participants' first fixations were fastest on general negative targets, whereas participants' first fixations on spider targets were slower in the spider fearful than in the nonfearful group. In addition, spider fearful participants made more nontarget fixations before fixating on a spider target than did nonfearful participants. Thus, we found that participants' overt attention was more quickly focused on general negative targets, whereas covert attentional processes enabled initial avoidance of fear-relevant (i.e. spider) stimuli. The present findings have important implications for research on attention and fear as they indicate that fearful individuals are not characterized by static attentional orienting toward threat but, under certain conditions, may avert attention from threat automatically. (PsycINFO Database Record (c) 2011 APA, all rights reserved)     

Multicomponent gas diffusion in nonuniform tubes

In many technical processes gas, multicomponent diffusion takes place in confinements that are rarely uniform in direction of their long axis (e.g., catalysts pores). Here, we show that in conical tubes multicomponent diffusion is hindered. This effect increases with ratio of inlet to outlet cone radius Λ, indifferent of the orientation of the tube. Based on the Maxwell–Stefan equations, predictive analytical solution for ideal multicomponent diffusion in slightly tapered ducts is developed. In two‐bulb diffusion experiments on a uniform tube, the results of Duncan and Toor (1962) were reproduced. Comparison of model and experiment shows that the solution presented here provides a reliable quantitative prediction of the temporal change of H₂, N₂, and CO₂‐concentration for both tube geometries, uniform and slightly conical. In the demonstrated case (Λ = 3.16), mass diffusion is 68% delayed. Thus, for gaseous diffusion in “real,” typically tapered pores the transport limitation is more serious than considered so far. © 2014 American Institute of Chemical Engineers AIChE J, 61: 1404–1412, 2015     

Increasing body satisfaction of body concerned women through evaluative conditioning using social stimuli.

Objective: Many women show weight and body concerns that leave them vulnerable to body dissatisfaction, lowered self-esteem, psychological distress, and eating disorders. This study tested whether body satisfaction could be increased by means of evaluative conditioning. Design: In the experimental condition (n = 26), women with low and high body concern completed a conditioning procedure in which pictures of their bodies were selectively linked to positive social stimuli (pictures of smiling faces). Pictures of control bodies were linked to neutral or negative social stimuli (neutral and frowning faces). In a control condition (n = 28), low and high body concerned women underwent a procedure in which pictures of their own body and of control bodies were randomly followed by positive, neutral, and negative social stimuli. Main Outcome Measures: Changes in body satisfaction and self-esteem before and after the conditioning task. Results: Women with high body concern demonstrated an increase in body satisfaction and global self-esteem when pictorial representations of their own bodies were associated with positive stimuli that signaled social acceptance. Conclusion: A simple conditioning procedure increased body satisfaction in healthy, normal weight women who were concerned about their shape and weight. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

面向3G LTE基站设计的预处理解决方案

长期演进（LTE）（参考文献1）蜂窝标准是3GPP3G演进的两个组成部分之一,另外一个是HSPA演进。如图1所示。LTE无线接入网络（RAN）规范被安排在2008年初完成,随后,一致性测试规范预计在2008年8月准备就绪。     

On Multicarrier Detection and Coherent CDMA Mobile Receiver Radio Systems with Joint Antenna Diversity

The combination of code division multiple access(CDMA) and multicarrier (MC) transmission techniques,termed MC-CDMA, is considered a promising alternative toconventional DS (direct sequence)-CDMA. For this reason, recent research activities haveconcentrated on the application of MC-CDMA to mobileradio systems. In this paper an MC-CDMA concept which iswell suited for mobile radio applications is described. The described MC-CDMA concept overcomesdisadvantages of previously proposed concepts. InMC-CDMA mobile radio systems, signal reception isimpaired by time-varying multipath propagation. Theimpairments can be reduced by applying diversitytechniques. Coherent receiver antenna diversity (CRAD)is especially attractive because only the signalprocessing at the receivers must be modified. In thiscommunication, the application of CRAD in combination withjoint detection (JD) techniques to the more criticaluplink of MC-CDMA mobile radio systems is investigated.It is explained that the deployment of JD techniques for CRAD is an effective countermeasure againstthe influence of the mobile radio channel on the systemperformance. Four JD techniques for CRAD which areapplicable to MC-CDMA are presented. Their performances are studied in bad urban, typical urban, andtypical macrocellular environments. It is shown thatMC-CDMA allows a favorable performance compared to otherCDMA concepts.     

Linoleic and linolenic acid as precursors of the cucumber flavor

Cucumber homogenates were incubated with¹⁴C-linolenic and¹⁴C-linoleic acid. Of the radioactivity, 0.3% was recovered in the fraction of flavor active aldehydes. The distribution of the specific racioactivity indicated that propanal,trans-2-hexenal andtrans-2,cis-6-nonadienal are related to 18∶3 and hexanal andtrans-2-nonenal to 18∶2. A pathway for the development of these compounds is discussed.     

Electrochemical Behavior of Single CuO Nanoparticles: Implications for the Assessment of their Environmental Fate

The electrochemical behavior of copper oxide nanoparticles is investigated at both the single particle and at the ensemble level in neutral aqueous solutions through the electrode‐particle collision method and cyclic voltammetry, respectively. The influence of Cl^? and NO₃^? anions on the electrochemical processes occurring at the nanoparticles is further evaluated. The electroactivity of CuO nanoparticles is found to differ between the two types of experiments. At the single‐particle scale, the reduction of the CuO nanoparticles proceeds to a higher extent in the presence of chloride ion than of nitrate ion containing solutions. However, at the multiparticle scale the CuO reduction proceeds to the same extent regardless of the type of anions present in solution. The implications for assessing realistically the environmental fate and therefore the toxicity of metal‐based nanoparticles in general, and copper‐based nanoparticles in particular, are discussed.     

GSM phase 2+ general packet radio service GPRS: Architecture, protocols, and air interface

Selective packet dropping policies have been used to reduce congestion and transmission of traffic that would inevitably be retransmitted. For data applications using best-effort services, packet dropping policies (PDPs) are congestion management mechanisms implemented at each intermediate node that decide, reactively or proactively, to drop packets to reduce congestion and free up precious buffer space. While the primary goal of PDPs is to avoid or combat congestion, the individual PDP designs can significantly affect application throughput, network utilization, performance fairness, and synchronization problems with multiple Transmission Control Protocol (TCP) connections. Scalability and simplicity are also important design issues. This article surveys the most important selective packet dropping policies that have been designed for best-effort traffic in ATM and IP networks, providing a comprehensive comparison between the different mechanisms.