EFFECT OF CHILLING AND FREEZING ON SURVIVAL OF VIBRIO PARAHAEMOLYTICUS ON FISH FILLETS1

Vibrio parahaemolyticus is a foodborne pathogen isolated from coastal waters of the United States, and from seafoods including fish. No information is available on the viability of V. parahaemolyticus on raw, chilled and frozen fish. A three‐strain mixture of V. parahaemolyticus was inoculated on fish fillets (pH 6.4) to obtain a bacterial load of 10⁴ (high) or 10³ (low) CFU/fillet, and stored at 4C or 8C for 9 days or at – 18C for seven weeks. At 4C and 8C, and at both levels of inoculation, V. parahaemolyticus survived on the fillets for the entire duration of the study. However, there was a significant reduction (P < 0.01) in V. parahaemolyticus population on the fillets by 9 days of storage. In the frozen fillets, there was a sharp decline (P < 0.01) in the population of V. parahaemolyticus by day 5 of storage. Although chilling and freezing significantly (P < 0.01) inactivated high numbers of V. parahaemolyticus on fish, they cannot be relied upon as a method to reduce V. parahaemolyticus on fish, since the time and magnitude of reduction depends on the initial load of the pathogen and the storage temperature.     

Effect of Oxide Defect Structure on the Electrical Properties of ZrO2

The defect structure of monoclinic ZrO₂ was studied by measuring the transfer numbers and electrical conductivity as functions of O₂ pressure and temperature. The data suggest a defect structure of doubly ionized oxygen vacancies at low pressures, i.e. <10⁻¹⁹ atm, and singly ionized oxygen interstitials at pressures >10⁻⁹ atm. Zirconia is primarily an ionic conductor below #700°C and an electronic conductor at 700° to 1000°C for 10⁻²²≤P_o2≤1 atm.     

Synthesis and characterization of stable aqueous dispersions of graphene

A stable aqueous dispersion (5 mg ml^?1) of graphene was synthesized by a simple protocol based on three-step reduction of graphene oxide (GO) dispersion synthesized using the modified version of Hummers and Offeman method. Reduction of GO was carried out using sodium borohydride, hydrazine hydrate and dimethyl hydrazine as reducing agents. The chemically synthesized graphene was characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), UV–visible absorption spectroscopy, Fourier transform infrared (FTIR) and Raman spectroscopy, thermogravimetric analysis (TGA), optical microscopy. The stability of aqueous dispersions of graphene was confirmed through zeta potential measurements and the negative zeta potentials of 55–60 mV were obtained indicating the high stability of aqueous graphene dispersions.     

Preparation and characterization of PAN–KI complexed gel polymer electrolytes for solid-state battery applications

The free standing and dimensionally stable gel polymer electrolyte films of polyacrylonitrile (PAN): potassium iodide (KI) of different compositions, using ethylene carbonate as a plasticizer and dimethyl formamide as solvent, are prepared by adopting ‘solution casting technique’ and these films are examined for their conductivities. The structural, miscibility and the chemical rapport between PAN and KI are investigated using X-ray diffraction, Fourier transform infrared spectroscopy and differential scanning calorimetry methods. The conductivity is enhanced with the increase in KI concentration and temperature. The maximum conductivity at 30°C is found to be 2.089 × 10^?5 S cm^?1 for PAN:KI (70:30) wt%, which is nine orders greater than that of pure PAN (< 10^?14 S cm^?1). The conductivity-temperature dependence of these polymer electrolyte films obeys Arrhenius behaviour with activation energy ranging from 0.358 to 0.478 eV. The conducting carriers of charge transport in these polymer electrolyte films are identified by Wagner’s polarization technique and it is found that the charge transport is predominantly due to ions. The better conducting sample is used to fabricate the battery with configuration K/PAN + KI/I₂+ C + electrolyte and good discharge characteristics of battery are observed.     

Quasi-cleavage fracture planes in spheroidized A533B steel

Electron backscattered diffraction (EBSD) has been used to acquire crystal orientation information around unusual microcracks induced by tensile deformation of notched specimens of spheroidized A533B steel. This unusual fracture mode has been called quasi‐cleavage and occurs at relatively low temperatures with fracture energies below that of the upper shelf. EBSD measurements on sectioned samples showed that the quasi‐cleavage cracks were intragranular. A two‐dimensional analysis technique was used in which EBSD measured crystal orientations were combined with secondary electron imaging to obtain the trace of the crack facet on the section plane. The measurements revealed that the observed crack facets were consistent with crack propagation along the {001} and {011} planes.     

Generalized probability model for estimating the proportion of the female population at different levels of fertility based on census sampling from a mixed population

The present exercise is an attempt to generalize a probability model referring to the waiting time distribution for conception in a mixed group of fecund females with two varying levels of fecundity, based on a census sample truncated at time T =t from the date of resumption of fecundity following an earlier conception (Biswas, 1975, Sankhya, Series B, 37, Part III). The generalization is made in two aspects, viz. instead of fixed conception rates, time-dependent rates decreasing with the age of the woman have been used. Additionally, unlike the model of Biswas (1975) consisting of two different fecund groups, three different levels of fecundity have been taken into consideration in this paper. Estimates of the proportion of fecund females over time, viz. π_i(t) (i = 1, 2, 3) are attempted by the method of maximum likelihood on the basis of a census sample truncated at time T = t under the generalized set up.     

Cryogenic forming behaviour of AW-6016-T4 sheet

The objective of this work is to study the cryogenic sheet metal forming behaviour of precipitation hardening AW-6016-T4. In this regard, the flow curves and forming limit curves were obtained by tension and Nakazima experimental testing methods in the temperature ranges from ?196 to 25 °C. It was found that strength and elongation increase with decreasing temperature. Small but perceived differences between microstructure of the material deformed at the room and cryogenic temperatures respectively were identified by electron backscatter diffraction (EBSD) analysis. However, no significant difference in the precipitation kinetics during continuous heating in the DSC has been observed. This study has demonstrated the potential of cryogenic forming by manufacturing a B-pillar part with 8 mm depth of side design element as compared to 6 mm at room temperature.     

GEOMETRIC PROGRAMMING. A NEW HYBRID ALGORITHM BASED ON LIAPUNOV CONCEPTS (MADLAP)

This article concerns the development of a simple yet very efficient heuristic procedure for optimizing the design or a reactor system employing continuous stirred tanks in series using Michaelis-Menten kinetics. The heuristic procedure employs a modified form or the well known Golden Search method to estimate the optimal number of reactors to be employed. The procedure was tested on an IBM personal computer under a wide range of values for three model parameters, namely the economy or scale factor, the scaled Michaelis-Menten constant and the desired degree of conversion to be attained. The results of this study indicated that the heuristic optimization procedure is capable of obtaining solutions that are either identical or very close to the actual optimal solutions in a very efficient manner.