Modified Galacto- or Fuco-Clusters Exploiting the Siderophore Pathway to Inhibit the LecA- or LecB-Associated Virulence of Pseudomonas aeruginosa

Galacto- and fuco-clusters conjugated with one to three catechol or hydroxamate motifs were synthesised to target LecA and LecB lectins of Pseudomonas aeruginosa (PA) localised in the outer membrane and inside the bacterium. The resulting glycocluster–pseudosiderophore conjugates were evaluated as Trojan horses to cross the outer membrane of PA by iron transport. The data suggest that glycoclusters with catechol moieties are able to hijack the iron transport, whereas those with hydroxamates showed strong nonspecific interactions. Mono- and tricatechol galactoclusters ( G1C and G3C ) were evaluated as inhibitors of infection by PA in comparison with the free galactocluster ( G0 ). All of them exhibited an inhibitory effect between 46 to 75 % at 100 μM, with a higher potency than G0 . This result shows that LecA localised in the outer membrane of PA is involved in the infection mechanism.     

Graduate Student Literature Review: Current recommendations and scientific knowledge on dairy goat kid rearing practices in intensive production systems in Canada,the United States,and France

Dairy goat kid rearing is the foundation of future milk production, yet little is known on this topic. References available to producers are limited, making it more difficult for dairy goat farms to reach their full production potential. This review paper aimed to identify the current recommendations on dairy goat kid rearing practices for intensive production systems and to assess whether the different recommendations were based on scientific literature. Recommendations on dairy goat kid rearing practices, from birth to weaning inclusively, were presented and compared between countries under similar intensive production systems, including Canada, the United States, and France. The different areas of rearing investigated included kidding management, colostrum management, liquid and solid feeding, health management, disbudding, housing, weaning, and growth monitoring. Gaps in the literature were identified in all areas except for disbudding. More research on the topic of goat kid raising practices would be beneficial to refine and validate current recommendations.     

Graduate Student Literature Review: The effect of housing systems on movement opportunity of dairy cows and the implications on cow health and comfort

Intensification within the dairy industry has led to an increase in use of more restrictive indoor housing systems (most commonly tiestalls and freestalls) and less use of the pasture-based housing systems used in past generations. These indoor housing systems are associated with not only a higher level of restriction to cow movement, but also with a higher prevalence of lameness and cow comfort issues on farm, which negatively affects cow welfare, public perceptions, and producer profitability. In looking for solutions to these issues, several studies have investigated different housing and management options that are less restrictive and increase cow movement opportunity. We hypothesized that movement opportunity, briefly summarized as the level of locomotor activity a cow is able to express in her given environment as well as the ease with which said movement can be expressed, can have a direct, substantial effect on cow comfort as well as cow leg and hoof health. Lying behaviors, which serve as common indicators of cow comfort, are affected not only by the ease of movement that comes with providing more movement opportunity within the lying environment, but also by the aforementioned improvements to leg health. This is particularly true regarding the ability to display different lying postures and to rise and lie down with ease. The complexity of the relationship between the different methods by which movement opportunity can be provided to dairy cows and the corresponding effects on outcome measures related to cow health and comfort is an area of research that warrants exploration. In this literature review, we sought to identify how level of movement opportunity, provided though different housing systems and management practices, affects cow hoof and leg, udder, and reproductive health, as well as the effects that it has on cow lying behaviors that are indicative of comfort.     

Effect of wobbling mass modeling on joint dynamics during human movements with impacts

In automated mechanical transmissions, engaging sleeve with clutch gear causes the switches of mechanical coupling modes of engaging-related parts, and impacts may happen to make the part speeds have discrete transitions during the engaging. The two characters make the engaging process exhibit both continuity and discreteness. The aim of the paper is to develop a high-fidelity model to capture the trajectories of the parts in the transmission. We treat the engaging as a two-phase process—sleeve first interacting with synchro ring and then with clutch gear. The part movements under a certain constraint are governed by multibody dynamics, and the speed jumps caused by impacts are described using the Poisson coefficient of restitution. To couple the continuous evolutions and discrete transitions of the states, a hybrid automaton model is developed. This model allows for the characteristic analysis of the engaging process with multiple interaction cases between the engaging-related parts, and it is validated by bench tests. The effects of two key factors—the relative tooth position of sleeve and clutch gear and the shifting force—on engaging duration and impact are studied through numerical simulations.     

Evaluation of performance improvement by model predictive control in a renewable energy system with hydrogen storage

Nowadays islanded microgrids mostly rely on diesel generator. In order to reduce greenhouse emissions, two islanded microgrids with hydrogen storage have been installed and are currently working autonomously in Reunion Island and France. Energy management implemented on these stations fall in the myopic control category. This study aims to determine the performance improvement that could be achieve on such stations using model predictive control. Ability to supply to the loads and energy losses minimisation are our main objectives. Lifetime degradation is also taken in consideration. Simulations shows that significant improvement can be brought, with a 76% decrease of the station's defaults time and better fill rate of hydrogen tank and batteries. In the meantime, chemical and electrical losses are reduced by 38 and 11%, and the batteries degradations are decreased by around 1%. The relevance of taking into account the electrolyser and fuel cell degradations depends on the time-step of the control. These results give a performance target in order to implement a real-time model predictive control in the microgrids, and eventually can be used to better sizing of future microgrids with similar architecture.     

Characterization of a neutral atomic hydrogen source developed in the perspective of carbon materials etching study

This paper deals with the characterization of an atomic hydrogen source created in a radio-frequency reactor with a helicon configuration. To achieve this purpose, optical (laser induced fluorescence) and electrical (Langmuir probe) diagnostics are used to monitor the behavior of the species composing the plasma. The influence of pressure, gas composition, and impact of the magnetic fields (in the source and diffusion chambers) are investigated.     

Magnetization Reversal in CsNiIICrIII(CN)6 Coordination Nanoparticles: Unravelling Surface Anisotropy and Dipolar Interaction Effects

CsNiCr(CN)₆ coordination nanoparticles with sizes ranging from 6 to 30 nm are highly diluted in an organic polymer matrix. Their static and dynamic magnetic behaviour allows unravelling of surface anisotropy and interparticle dipolar interaction effects. The single magnetic domain critical size is thus evaluated to be around 22 nm with a blocking temperature of 21 K (at ν = 1 Hz) and an effective energy barrier for the reversal of the magnetization of 426 K.     

Statistical characterization of acceleration levels of random vibrations during transport

Random vibration tests are an efficient way to simulate the mechanical vibratory effects caused by transportation. The usual method is only concerned with the frequency distribution pattern of the signal using the average power spectral density. This work offers an additional method based on detailed analysis of instantaneous acceleration levels of a real road transport, which enables modelling of the statistical distribution of these levels. Continuous recording of acceleration signal all along the journey permits confirmation that this statistical distribution is not a Gaussian distribution but a modified Gaussian distribution, for which parameters are estimated and discussed. Therefore, it is possible to evaluate the transport severity by working out the appearance probability of acceleration levels greater than a fixed threshold and also the statistical moments, i.e. second order moment which gives the root mean square value together with fourth order moment (kurtosis) which evaluates the difference between the experimental distribution and the Gaussian distribution. Copyright © 2011 John Wiley & Sons, Ltd.