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1.
Emma Layton Anna-Marie Fairhurst Sam Griffiths-Jones Richard K. Grencis Ian S. Roberts 《International journal of molecular sciences》2020,21(23)
In eukaryotes, microRNAs (miRNAs) have roles in development, homeostasis, disease and the immune response. Recent work has shown that plant and mammalian miRNAs also mediate cross-kingdom and cross-domain communications. However, these studies remain controversial and are lacking critical mechanistic explanations. Bacteria do not produce miRNAs themselves, and therefore it is unclear how these eukaryotic RNA molecules could function in the bacterial recipient. In this review, we compare and contrast the biogenesis and functions of regulatory RNAs in eukaryotes and bacteria. As a result, we discovered several conserved features and homologous components in these distinct pathways. These findings enabled us to propose novel mechanisms to explain how eukaryotic miRNAs could function in bacteria. Further understanding in this area is necessary to validate the findings of existing studies and could facilitate the use of miRNAs as novel tools for the directed remodelling of the human microbiota. 相似文献
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Xunwang Shi Xiuhan Yu Caiwen Nie Fengji Li Sam Zhang 《Ceramics International》2021,47(15):21546-21553
Color films are widely used for visual effect as well as for their functional properties. To date, however, synthesizing thin films with desired color remains challenging. In this work, AlN color films are deposited on Si wafers by precise control of the deposition time for different thickness during reactive magnetron sputtering from an Al target in Ar/N2 atmosphere. The thickness, morphology, structure, composition and color index are carefully examined by field emission scanning electron microscopy, atomic force microscopy, grazing incidence X-ray diffraction, X-ray photoelectron spectrometry and colorimeter, respectively. As the film thickness changes from 57 nm to 165 nm, the film exhibits purple, indigo, blue, green, yellow, orange and red in color. These colors repeat in the same order when the thickness goes over 165 nm. Once the thickness exceeds 467 nm, overlapping of colors takes place. The mechanisms are elucidated. 相似文献
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Selvasundarasekar Sam Sankar Arumugam Rathishkumar Kathiresan Geetha Subrata Kundu 《International Journal of Hydrogen Energy》2021,46(17):10366-10376
Electrospinning (ES) is a most reliable method for synthesizing one dimensional (1D) fibrous material. Fibrous materials are having peculiar interest owing to their fascinating properties. For efficient hydrogen fuel generation, electrocatalytic water splitting is one of the finest way of producing hydrogen in a pure form. But it is encountered by the counter oxygen evolution reaction (OER) in more often. As of now, noble metal based catalysts are utilized in the commercial sector. Some of the disadvantages associated with the noble materials are restrict their usage commercially. To address this issue, herein, we have synthesized One dimensional (1D), hydrated porous cobalt phosphate fibrous network by an ES method and act as an electrocatalyst for OER in both alkaline and neutral media for the first time, which exhibits an overpotential of 245 and 457 mV respectively at a current density of 10 mAcm?2 with astonishing stability. 相似文献
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Suying Xu Adam C. Sedgwick Souad A. Elfeky Wenbo Chen Ashley S. Jones George T. Williams A. Toby A. Jenkins Steven D. Bull John S. Fossey Tony D. James 《Frontiers of Chemical Science and Engineering》2020,14(1):112
A boronic acid-based anthracene fluorescent probe was functionalised with an acrylamide unit to incorporate into a hydrogel system for monosaccharide detection. In solution, the fluorescent probe displayed a strong fluorescence turn-on response upon exposure to fructose, and an expected trend in apparent binding constants, as judged by a fluorescence response where D-fructose>D-galactose>D-mannose>D-glucose. The hydrogel incorporating the boronic acid monomer demonstrated the ability to detect monosaccharides by fluorescence with the same overall trend as the monomer in solution with the addition of D-fructose resulting in a 10-fold enhancement (≤0.25 mol/L). 相似文献
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Sam Safran 《Israel journal of chemistry》2015,55(11-12):1160-1163
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Isothermal nucleic-acid amplification methods such as Loop-Mediated isothermal AMPlification (LAMP) are increasingly appealing alternatives to PCR for use in portable diagnostic system due to the low cost, weight, and power requirements of the instrumentation. As such, interest in developing new probes and other functionality based on the LAMP reaction has been intense. Here, we report on the development of duplexed LAMP assays for pathogen detection using spectrally unique Assimilating Probes. As proof of principle, we used a reaction for Salmonella enterica as a model coupled with a reaction for λ-phage DNA as an internal control, as well as a duplexed assay to sub-type specific quarantine strains of the bacterial wilt pathogen Ralstonia solanacearum. Detection limits for bacterial DNA analyzed in individual reactions was less than 100 genomic equivalents in all cases, and increased by one to two orders of magnitude when reactions were coupled in duplexed formats. Even so, due to the more robust activity of newly available strand-displacing polymerases, the duplexed assays reported here were more powerful than analogous individual reactions reported only a few years ago, and represent a significant advance for incorporation of internal controls to validate assay results in the field. 相似文献
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