Pre-peritoneal bisaccular umbilical and subcutaneous abscess after laparoscopic appendectomy

Laparoscopic surgery has gained much interest since its advent the late eighties. A 36-year-old patient underwent laparoscopic appendicectomy via the transumbilical route. She was rehospitalized three times thereafter for subumblical pain. At day +48, an abscess was evacuated at the point of the umbilical trocar insertion. This hour-glass shaped abscess had a preperitoneal and subcutaneous localization, blocked by the epiploic reaction. The infection this patient developed is an unusual clinical finding as pain was localized at a distance. The umbilical site and the preperitoneal subcutaneous nature of the abscess are also unusual. This case emphasizes the gravity of post-operative abscess of the abdominal wall and the necessity for adequate prophylaxy.     

The putative tumor suppressor gene on chromosome 5q for hepatocellular carcinoma is distinct from the MCC and APC genes

We have previously shown that the tumor suppressor gene for hepatocellular carcinoma (HCC) without cirrhosis may be located on chromosome 5q35-qter. In this study, we analyzed nine cases of primary HCC without cirrhosis using probes from the MCC and APC genes, which are in the region 5q21-22. None of the informative cases had allele loss detected by these probes, whereas the probe lambda MS8 for the region 5q35-qter showed allele loss in six out of six informative cases. The results confirm that the putative tumor suppressor gene for HCC without cirrhosis on chromosome 5q is distinct from the MCC and APC genes.     

Effects of the cryogenic cooling on the fatigue strength of the AISI 304 stainless steel ground components

For environmental considerations, the substitution of the conventionally used oil-based grinding fluids has nowadays become strongly recommended. Although several alternatives have been proposed, cryogenic cooling by liquid nitrogen is the non-polluting coolant that has been given relatively more attention because of its very low temperature. In this investigation, in order to contribute to developing this promising cooling mode, its beneficial effects on the ground surface integrity of the AISI 304 stainless steel and their consequences on the fatigue lifetime are explored. Results of this investigation show that grinding under cryogenic cooling mode generates surfaces with lower roughness, less defects, higher work hardening and less tensile residual stresses than those obtained on surfaces ground under oil-based grinding fluid. These surface enhancements result into substantial improvements in the fatigue behaviour of components ground under this cooling mode. An increasing rate of almost 15% of the endurance limit at 2 × 10⁶ cycles could be realized. SEM analyses of the fatigue fracture surfaces have shown that the fatigue cracks observed on the specimens ground under cryogenic cooling are shorter (i.e., 30-50 μm) than those generated under oil-based cooling mode (i.e., 150-200 μm). The realized improvements in the surface integrity and in the fatigue behaviour are thought to be related to the reduction of the grinding zone temperature observed under cryogenic cooling, as no significant differences between the grinding force components for both cooling modes have been observed.     

Erosion rate correlations of a pipe protruded in an abrupt pipe contraction

Erosion is one of the most serious problems in various gas and liquid flow passages such as flow in pipes, pumps, turbines, compressors and many other devices. Sand presence causes loss of pipe wall thickness that can lead to pipe erosion, frequent failures and loss of expensive production time. The importance of this problem is mainly due to many related engineering applications, viz. heat exchangers. In order to reduce the frequency of such pipe erosions, caps in the form of replaceable pipes are protruded in the sudden contraction regions which are exposed to most of the serious erosion rates. In the present work, numerical investigation of the erosion of a pipe protruded in a sudden contraction is presented. The turbulent, steady, 2-D axi-symmetric flow inside an axi-symmetric abrupt contraction pipe with a pipe protrusion embedded in it was solved by steady-state time averaged conservation equations of mass and momentum along with two equation model for turbulence. Particles are tracked using Lagrangian particle tracking. An erosion model was employed to investigate the erosion phenomena for the given geometry. The influence of the different parameters such as the inlet flow velocity (3–10 m/s), the particle diameter (10–400 μm), the protruded pipe geometry (thickness T=1–5 mm and depth H=2–5 mm) and the pipe contraction ratio (Cr=0.25–0.5) on the erosion of pipe protrusion was investigated. Correlations for the influence of inlet flow velocity, depth and thickness of the protruded pipe on the erosion rate are presented.     

Depollution of atmospheric emissions of wood pyrolysis furnaces

The wood carbonization in Tunisia consists essentially of traditional activity using charcoaling stacks and pits characterized by high atmospheric pollution and poor energy conversion. Indeed, 70% of the initial mass of anhydrous wood are found in the vapor as aerosols, polluting and toxic gases and complex condensable organic compounds that can cause a substantial pollution of air, ground and water. Several processes of treatment and energy valorization of such effluents were proposed, but the incineration remains at present the most promising technique of depollution. The results show that the incineration, at about 1000°C, of wood carbonization smokes allows the destruction of 99% of the mass of pollutants except CO₂ and the reduction of polluting gas emission. The possible valorization of the smoke’s energy in the exit of the incinerator enhances the thermal efficiency of the process.     

On the problem of k-coverage in mission-oriented mobile wireless sensor networks

The problem of sensor deployment to achieve k-coverage of a field, where every point is covered by at least k sensors, is very critical in the design of energy-efficient wireless sensor networks (WSNs). It becomes more challenging in mission-oriented WSNs, where sensors have to move in order to k-cover a region of interest in the field. In this type of network, there are multiple missions (or monitoring tasks) to be accomplished, each of which has different requirements, particularly, in terms of coverage. In this paper, we consider the problem of k-coverage in mission-oriented mobile WSNs which we divide into two sub-problems, namely sensor placement and sensor selection. The sensor placement problem is to identify a subset of sensors and their locations in a region of interest so it is k-covered with a small number of sensors. The sensor selection problem is to determine which sensors should move to the above-computed locations in the region while minimizing the total energy consumption due to sensor mobility and communication. Specifically, we propose centralized and distributed approaches to solve the k-coverage problem in mission-oriented mobile WSNs. Our solution to the sensor placement problem is based on Helly’s Theorem and the geometric analysis of the Reuleaux triangle. First, we consider a deterministic (or disk) sensing model, where the sensing range is modeled as a disk. Then, based on the above analysis, we address the k-coverage problem using a more realistic sensing model, known as probabilistic sensing model. The latter reflects the stochastic nature of the characteristics of the sensors, namely sensing and communication ranges. Our centralized and distributed protocols enable the sensors to move toward a region of interest and k-cover it with a small number of sensors. Our experiments show a good match between simulation and analytical results. In particular, simulation results show that our solution to the k-coverage problem in mission-oriented mobile WSNs outperforms an existing one in terms of the number of sensors needed to k-cover a region of interest in the field and their total energy consumption due to communication, sensing, and mobility for the correct operation of the protocol.     

TP63 Is Significantly Upregulated in Diabetic Kidney

The role of tumor protein 63 (TP63) in regulating insulin receptor substrate 1 (IRS-1) and other downstream signal proteins in diabetes has not been characterized. RNAs extracted from kidneys of diabetic mice (db/db) were sequenced to identify genes that are involved in kidney complications. RNA sequence analysis showed more than 4- to 6-fold increases in TP63 expression in the diabetic mice’s kidneys, compared to wild-type mice at age 10 and 12 months old. In addition, the kidneys from diabetic mice showed significant increases in TP63 mRNA and protein expression compared to WT mice. Mouse proximal tubular cells exposed to high glucose (HG) for 48 h showed significant decreases in IRS-1 expression and increases in TP63, compared to cells grown in normal glucose (NG). When TP63 was downregulated by siRNA, significant increases in IRS-1 and activation of AMP-activated protein kinase (AMPK (p-AMPK-Th¹⁷²)) occurred under NG and HG conditions. Moreover, activation of AMPK by pretreating the cells with AICAR resulted in significant downregulation of TP63 and increased IRS-1 expression. Ad-cDNA-mediated over-expression of tuberin resulted in significantly decreased TP63 levels and upregulation of IRS-1 expression. Furthermore, TP63 knockdown resulted in increased glucose uptake, whereas IRS-1 knockdown resulted in a decrease in the glucose uptake. Altogether, animal and cell culture data showed a potential role of TP63 as a new candidate gene involved in regulating IRS-1 that may be used as a new therapeutic target to prevent kidney complications in diabetes.     

Design and simulation of a novel metallic microgripper using vibration to release nano objects actively

In this study, we investigate a novel metallic microgripper which is able to grasp and transport nano particles (nano tubes/wires) and release them on desirable substrate by vibrating the gripper arms. This microgripper consists of a chevron actuator to grip nano object electrothermally and interdigited comb drive systems to generate vibration at the gripper arms electrostatically. Metallic (nickel) properties enable the chevron actuator to close the gap and pick the nano particle at low voltage and temperature. In order to reduce the out of plane bending during operation and also increase the gripping force, thickness of the nickel layer must be increased, hence electroplating process is proposed for deposition of nickel layer. To generate vibration at the end effectors, comb drive systems are stimulated by applying two voltage signals at desired resonant frequency to the stators. Practically, by sweeping the frequency of these signals around the resonant frequency the end effectors start vibrating. The vibration results in overcoming the adhesion forces due to inertial effects.     

Metallo-organic deposition of tungsten oxide films from alkylammonium tungstate solutions

This paper describes a simple and inexpensive metallo-organic deposition (MOD) process for forming electrochromic tungsten oxide (WO₃) films on glass. The thin films of WO₃ were made by air firing (500–700°C) films from xylene/2-propanol solutions of bis-(di-n-octylammonium) tetratungstate, [(n-C₈H₁₇)₂NH₂]₂[W₄O₁₃]. The process coats glass with undoped films ranging in colour from faint yellow to dark brown, and can be used to make gradients of these colours. The colour is determined by the firing parameters and results from residual carbon and tungsten suboxides in the film due to incomplete firing. Increased firing temperatures or longer firing times removes the carbon and produces films with higher crystallinity. Electrochemical doping with acid (H⁺) switches the colour gradient films to a uniformly blue colour.