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1.
Biopolymer sequencing with mass spectrometry has become increasingly important and accessible with the development of matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI). Here we examine the use of sequential digestion for the rapid identification of proteolytic fragments, in turn highlighting the general utility of enzymatic MALDI ladder sequencing and ESI tandem mass spectrometry. Analyses were performed on oligonucleotides ranging in size from 2 to 50 residues, on peptides ranging in size from 7 to 44 residues and on viral coat proteins. MALDI ladder sequencing using exonuclease digestion generated a uniform distribution of ions and provided complete sequence information on the oligonucleotides 2-30 nucleic acid residues long. Only partial sequence information was obtained on the longer oligonucleotides. C-terminal peptide ladder sequencing typically provided information from 4 to 7 amino acids into the peptide. Sequential digestion, or endoprotease followed by exoprotease exposure, was also successfully applied to a trypsin digest of viral proteins. Analysis of ladder sequenced peptides by LCMS generated less information than in the MALDI-MS analysis and ESI-MS2 normally provided partial sequence information on both the small oligonucleotides and peptides. In general, MALDI ladder sequencing offered information on a broader mass range of biopolymers than ESI-MS2 and was relatively straightforward to interpret, especially for oligonucleotides. 相似文献
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Programming and Computer Software - In the biomedical domain, diagrammatical models have been extensively used to describe and understand the behaviour of biological organisms (biological agents)... 相似文献
3.
We describe Social Reader, a feed-reader-plus-social-network aggregator that mines comments from social media in order to display a user’s relational neighborhood as a navigable social network. Social Reader’s network visualization enhances mutual awareness of blogger communities, facilitates their exploration and growth with a fully dragn- drop interface, and provides novel ways to filter and summarize people, groups, blogs and comments. We discuss the architecture behind the reader, highlight tasks it adds to the workflow of a typical reader, and assess their cost. We also explore the potential of mood-based features in social media applications. Mood is particularly relevant to social media, reflecting the personal nature of the medium. We explore two prototype mood-based features: colour coding the mood of recent posts according to a valence/arousal map, and a mood-based abstract of recent activity using image media. A six week study of the software involving 20 users confirmed the usefulness of the novel visual display, via a quantitative analysis of use logs, and an exit survey. 相似文献
4.
Dinh Phung Brett Adams Svetha Venkatesh Mohan Kumar 《Pervasive and Mobile Computing》2009,5(6):714-733
The sensing context plays an important role in many pervasive and mobile computing applications. Continuing from previous work [D. Phung, B. Adams, S. Venkatesh, Computable social patterns from sparse sensor data, in: Proceedings of First International Workshop on Location Web, World Wide Web Conference (WWW), New York, NY, USA, 2008, ACM 69–72.], we present an unsupervised framework for extracting user context in indoor environments with existing wireless infrastructures. Our novel approach casts context detection into an incremental, unsupervised clustering setting. Using WiFi observations consisting of access point identification and signal strengths freely available in office or public spaces, we adapt a density-based clustering technique to recover basic forms of user contexts that include user motion state and significant places the user visits from time to time. High-level user context, termed rhythms, comprising sequences of significant places are derived from the above low-level context by employing probabilistic clustering techniques, latent Dirichlet allocation and its n-gram temporal extension. These user contexts can enable a wide range of context-ware application services. Experimental results with real data in comparison with existing methods are presented to validate the proposed approach. Our motion classification algorithm operates in real-time, and achieves a 10% improvement over an existing method; significant locations are detected with over 90% accuracy and near perfect cluster purity. Richer indoor context and meaningful rhythms, such as typical daily routines or meeting patterns, are also inferred automatically from collected raw WiFi signals. 相似文献
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Application of common transformers faults diagnosis methods on biodegradable oil-filled transformers
N. A. Muhamad B. T. Phung T. R. Blackburn 《Electrical Engineering (Archiv fur Elektrotechnik)》2012,94(4):207-216
Due to environmental concerns regarding the use of mineral oil, biodegradable oil is increasingly being used as an alternative dielectric fluid. This paper presents results of experiments performed in the laboratory on the use of biodegradable oils for transformer application. The investigations cover two important diagnostic techniques for insulation assessment: (i) an investigation of partial discharge (PD) activity and characteristics in such oils and (ii) an investigation of the standard hydrocarbon dissolved gas products produced due to transformer faults. The fundamental aim of the investigation was to provide information as to whether the existing analysis techniques of PD fault pattern recognition and dissolved gas analysis methods, developed for mineral oil, are valid when used for insulation assessment with biodegradable transformer oil. The experiments were conducted on test samples to simulate three common types of transformer faults: low-energy PDs, high-energy arcing/sparking, and overheating. For the purpose of comparison, the same tests were performed using a mineral oil (Shell Diala MX) and a biodegradable oil (Envirotemp FR3). Also, each oil type was tested at three different moisture levels and with or without the presence of solid insulating materials (pressboard). PD activity was monitored using the standard IEC60270 phase-resolved analysis method. The fault gases produced were extracted and analysed by standard gas chromatography methods. Test results indicate that the PD phase resolved patterns are, in general, similar for the two oil types and thus existing PD pattern interpretations can be used to distinguish different types of PD faults, e.g. corona versus surface discharges. However, the values of various discharge quantities (PD magnitudes, repetition rate, current, etc) are very different under the same test condition. The quantities and the trend of dissolved gases for faults in biodegradable oil are substantially different compared with mineral oil. For the PD fault, biodegradable oil was found to release only a limited number of gases. For arcing and overheating faults, the main key gas produced is different between the two oils. Also, the presence of cellulosic materials such as pressboard influences the extent of hydrocarbon gases dissolved in the oil. It was found that for biodegradable oil, fewer amounts of gases are produced as the oil gets wetter. 相似文献
8.
Detection of cross-channel anomalies 总被引:1,自引:1,他引:0
Duc-Son Pham Budhaditya Saha Dinh Q. Phung Svetha Venkatesh 《Knowledge and Information Systems》2013,35(1):33-59
The data deluge has created a great challenge for data mining applications wherein the rare topics of interest are often buried in the flood of major headlines. We identify and formulate a novel problem: cross-channel anomaly detection from multiple data channels. Cross-channel anomalies are common among the individual channel anomalies and are often portent of significant events. Central to this new problem is a development of theoretical foundation and methodology. Using the spectral approach, we propose a two-stage detection method: anomaly detection at a single-channel level, followed by the detection of cross-channel anomalies from the amalgamation of single-channel anomalies. We also derive the extension of the proposed detection method to an online settings, which automatically adapts to changes in the data over time at low computational complexity using incremental algorithms. Our mathematical analysis shows that our method is likely to reduce the false alarm rate by establishing theoretical results on the reduction of an impurity index. We demonstrate our method in two applications: document understanding with multiple text corpora and detection of repeated anomalies in large-scale video surveillance. The experimental results consistently demonstrate the superior performance of our method compared with related state-of-art methods, including the one-class SVM and principal component pursuit. In addition, our framework can be deployed in a decentralized manner, lending itself for large-scale data stream analysis. 相似文献
9.
Sunil Kumar Gupta Dinh Phung Brett Adams Svetha Venkatesh 《Data mining and knowledge discovery》2013,26(1):57-97
Joint modeling of related data sources has the potential to improve various data mining tasks such as transfer learning, multitask clustering, information retrieval etc. However, diversity among various data sources might outweigh the advantages of the joint modeling, and thus may result in performance degradations. To this end, we propose a regularized shared subspace learning framework, which can exploit the mutual strengths of related data sources while being immune to the effects of the variabilities of each source. This is achieved by further imposing a mutual orthogonality constraint on the constituent subspaces which segregates the common patterns from the source specific patterns, and thus, avoids performance degradations. Our approach is rooted in nonnegative matrix factorization and extends it further to enable joint analysis of related data sources. Experiments performed using three real world data sets for both retrieval and clustering applications demonstrate the benefits of regularization and validate the effectiveness of the model. Our proposed solution provides a formal framework appropriate for jointly analyzing related data sources and therefore, it is applicable to a wider context in data mining. 相似文献
10.
Yuhao Huang Akhil Garg Saeed Asghari Xiongbin Peng My Loan Phung LE 《International Journal of Hydrogen Energy》2018,43(1):341-353
Energy production systems such as proton-exchange membrane fuel cell (PEMFC) has a promising future in the cleaner energy market due to zero emissions. Rubber pad forming (RPF) process of metallic bipolar plates of PEMFCs is gaining attention among the researchers. Studies based on design of experiments have been conducted to find the crucial parameters of the forming process. These methods are based on the assumptions of the model structure, correlated residuals, etc., which can cause uncertainty in estimation ability of the model on unseen data. Therefore, the present study focuses on the design of robust models of these parameters for PEMFCs using an optimization approach of genetic programming (GP). The inputs from the experiments considered in GP are radius, the friction coefficient, the filling factor and the minimum thickness. Experiments on PEMFCs validates the performance of the GP models. Further, the relationships between the two inputs and the three outputs for PEMFCs are generated as well as the contributions of each input to each of the output. Optimization of the models generated by GP can further determine the forming quality of metallic bipolar plates of PEMFCs by an appropriate setting of the two inputs. 相似文献