Plant Copper Amine Oxidases: Key Players in Hormone Signaling Leading to Stress-Induced Phenotypic Plasticity

Polyamines are ubiquitous, low-molecular-weight aliphatic compounds, present in living organisms and essential for cell growth and differentiation. Copper amine oxidases (CuAOs) oxidize polyamines to aminoaldehydes releasing ammonium and hydrogen peroxide, which participates in the complex network of reactive oxygen species acting as signaling molecules involved in responses to biotic and abiotic stresses. CuAOs have been identified and characterized in different plant species, but the most extensive study on a CuAO gene family has been carried out in Arabidopsis thaliana. Growing attention has been devoted in the last years to the investigation of the CuAO expression pattern during development and in response to an array of stress and stress-related hormones, events in which recent studies have highlighted CuAOs to play a key role by modulation of a multilevel phenotypic plasticity expression. In this review, the attention will be focused on the involvement of different AtCuAOs in the IAA/JA/ABA signal transduction pathways which mediate stress-induced phenotypic plasticity events.     

Design and Characterization of a New pVII Combinatorial Phage Display Peptide Library for Protease Substrate Mining Using Factor VII Activating Protease (FSAP) as Model

We describe a novel, easy and efficient combinatorial phage display peptide substrate-mining method to map the substrate specificity of proteases. The peptide library is displayed on the pVII capsid of the M13 bacteriophage, which renders pIII necessary for infectivity and efficient retrieval, in an unmodified state. As capture module, the 3XFLAG was chosen due to its very high binding efficiency to anti-FLAG mAbs and its independency of any post-translational modification. This library was tested with Factor-VII activating protease (WT-FSAP) and its single-nucleotide polymorphism variant Marburg-I (MI)-FSAP. The WT-FSAP results confirmed the previously reported Arg/Lys centered FSAP cleavage site consensus as dominant, as well as reinforcing MI-FSAP as a loss-of-function mutant. Surprisingly, rare substrate clones devoid of basic amino acids were also identified. Indeed one of these peptides was cleaved as free peptide, thus suggesting a broader range of WT-FSAP substrates than previously anticipated.     

A novel compact circuit for 4-PAM energy-efficient high speed interconnect data transmission and reception

Transmission of signals, whether on-chip or off-chip, places severe constraints on timing and extracts a large price in energy. New silicon device technologies, such as back-plane CMOS, provide a programmable and adaptable threshold voltage as an additional tool that can be used for low power design. We show that one particularly desirable use of this freedom is energy-efficient high-speed transmission across long interconnects using multi-valued encoding. Our multi-valued CMOS circuits take advantage of the threshold voltage control of the transistors, by using the signal-voltage-to-threshold-voltage span, in order to make area-efficient implementations of 4-PAM (pulse amplitude modulation) transceivers operating at high speed. In a comparison of a variety of published technologies, for signal transmission with interconnects of 10-15 mm length, we show up to 50% improvement in energy for on-chip signal transmission over binary encoding together with higher limits for operating speeds without a penalty in circuit noise margin.     

Designing linear distributed algorithms with memory for fast convergence

Motivated by both distributed computation and decentralized control applications, we studied the distributed linear iterative algorithms with memory. Specifically, we showed that the system of linear equations G x = b can be solved through a distributed linear iteration for arbitrary invertible G using only a single memory element at each processor. Further, we demonstrated that the memoried distributed algorithm can be designed to achieve much faster convergence than a memoryless distributed algorithm. Two small simulation examples were included to illustrate the results. Copyright © 2011 John Wiley & Sons, Ltd.     

Security concepts for the dynamics of autonomous vehicle networks

The secure operation of autonomous vehicle networks in the presence of adversarial observation is examined, in the context of a canonical double-integrator-network (DIN) model. Specifically, we study the ability of a sentient adversary to estimate the full network’s state, from noisy local measurements of vehicle motions. Algebraic, spectral, and graphical characterizations are provided, which indicate the critical role of the inter-vehicle communication topology and control scheme in achieving security.     

Evolution of Surface Morphology in Laser-Dressed Alumina Grinding Wheel Material

An approach to laser dressing of alumina grinding wheels is proposed based on solidification microstructures associated with rapid cooling rates obtained in laser surface processing. Laser dressing of alumina grinding wheels forms surface microstructures characterized by multifaceted grains that are expected to facilitate the micro-scale material removal during precision machining. A detailed investigation of variation of grain size and melt depth with laser fluence is conducted. The results are correlated with calculated cooling rates derived from a thermal model. In addition, based on microscopic observations, the formation of surface grains by stacking of individual multifaceted grains formed during laser dressing is suggested.     

A simulation framework for measuring robustness of incentive mechanisms and its implementation in reputation systems

In game theoretical analysis of incentive mechanisms, all players are assumed to be rational. Since it is likely that mechanism participants in the real world may not be fully rational, such mechanisms may not work as effectively as in the idealized settings for which they were designed. Therefore, it is important to evaluate the robustness of incentive mechanisms against various types of agents with bounded rational behaviors. Such evaluations would provide us with the information needed to choose mechanisms with desired properties in real environments. In this article, we first propose a general robustness measure, inspired by research in evolutionary game theory, as the maximal percentage of invaders taking non-equilibrium strategies such that the agents sustain the desired equilibrium strategy. We then propose a simulation framework based on evolutionary dynamics to empirically evaluate the equilibrium robustness. The proposed simulation framework is validated by comparing the simulated results with the analytical predictions based on a modified simplex analysis approach. Finally, we implement the proposed simulation framework for evaluating the robustness of incentive mechanisms in reputation systems for electronic marketplaces. The results from the implementation show that the evaluated mechanisms have high robustness against a certain non-equilibrium strategy, but is vulnerable to another strategy, indicating the need for designing more robust incentive mechanisms for reputation management in e-marketplaces.     

Stabilizing adaptive controller for uncertain dynamical systems: An LMI approach

Adaptive stabilization of a class of linear systems with matched and unmatched uncertainties is considered in this paper. The proposed controller indeed stabilizes the uncertain system for any positive values of its non-adaptive gain that may be tuned to enhance dynamic response of system. The performance of uncertain system along with the Algebraic Riccati Equation that arises from the adaptive stabilizing controller is now formulated as a multi-objective Linear Matrix Inequality optimization problem. The decay rate and a factor governing the ultimate bound of the system states are considered to characterize the closed loop system performance. Finally, the effectiveness of the proposed controller is illustrated via stabilizing a mass-spring system. Recommended by Editorial Board member Gang Tao under the direction of Editor Young Il Lee. The authors would like to thank the reviewers for their valuable comments and suggestions that have improved the quality of this paper. Sandip Ghosh received the B.E. in Electrical Engineering from Bengal Engineering College (D.U.), Howrah, and Master in Control System Engineering from Jadavpur University, Kolkata, India, in 1999 and 2003 respectively. Presently he is pursuing the Ph.D. degree at Indian Institute of Technology, Kharagpur, India. His research interests include adaptive control, robust control and control of time-delay systems. Sarit K. Das is a Professor of Electrical Engineering Department, Indian Institute of Technology, Kharagpur, India. He received the Ph.D. degree in 1985 from the same department. His research interests include design of periodic controller, decoupling of multivariable systems, modeling and robust control of complex systems. Goshaidas Ray is a Professor of Electrical Engineering Department, Indian Institute of Technology, Kharagpur, India. He received the Ph.D. degree in 1982 from Indian Institute of Technology Delhi, India. His research interests include modeling, estimation, model-based control, intelligent control, robotic systems and distributed control systems.     

Design and analysis of miniaturized all-optical binary to gray code converter using Y-shaped plasmonic waveguide for optical processors

In this paper, an all-optical miniaturized binary to gray code converter is designed and analyzed. The all-optical domain is now an alternative for electronic devices, where performance and speed are the key issues. Code converters are significantly used in digital data transmission in the areas of error detection and correction. Gray code is one of the cyclic codes, where the cyclic shift of each codeword is also a code word. An all-optical XOR gate, realized using a Y-shaped power combiner is used in this design to generate the desired gray code from the given binary code. The insertion loss and extinction ratio parameters are found to be 0.347 dB and 22.26 dB, respectively. The entire simulation is carried out using finite-difference time-domain method. The obtained practical results are verified mathematically using MATLAB.